CAZyme Information: MGYG000003137_04758

Basic Information

• Species: Bradyrhizobium sp000015165
• Lineage: Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Xanthobacteraceae; Bradyrhizobium; Bradyrhizobium sp000015165
• CAZyme ID: MGYG000003137_04758
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
745	MGYG000003137_23|CGC2	80056.26	10.2355

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003137	8388709	MAG	United States	North America

• Gene Location: Start: 129000; End: 131237 Strand: -

Full Sequence      

MSEGQTSGNGRMTEPDTPSSLPGLTRPSTAACENGVDARVKPGHDERRGRVAKRLAAIGL
TLIVVVATALTAYTISLGPLPLQDAKALSTTVVDRHGKLLRAYAMADGRWRLPVSAQKDV
DPTYLKLLFAYEDQRFYDHHGVDPRALARAAIQFGSHGHIVSGGSTITMQLARLLEPRRQ
RSLHAKLRQIVRALELERKLGKDEILDLYLTLAPYGGNLEGIRAASIAYFGKEPKRLSLA
EAALLVALPQSPETRRLDRHPEAARRGRDRVLDRMVSENVISAEDAAQAKAQPVPAFRKP
MPILAPHAADQATAVVKDRPIIRLTLDASLQKVLEPLARDRAAALGPNISVGIIVIDNDS
GDVLAHVGSADYFDERRAGQVDMTRAVRSPGSTLKPFIYGLAFEDGFVHPESLIEDRPVR
FGSYAPENFDMTFQGTVPVRKALQLSLNVPAIVLLDRVGASRLSSRLKQAGGNLVLPKDE
APGLAMGLGGVGVTLQDLAQLYAGFPRLGTTKPLREIMTAEVLTAEDADTREPMRLMDPA
AAWQVGNVLLGTPPPENAARNRIAFKTGTSYGYRDAWSVGFDGRMTIGVWVGRPDGAPVP
GLIGRVAAAPILFDAFARSGKLPAALPKPPKGALIAGNSKLPLPLRRFRPIGELLRTGAE
QAPHIQFPLNGSRIDVDRTAGPEAAVMPVKVSGGVLPLTMLVNGLPVGEIDSRRQRLIDP
PGPGFARLTVIDATGAADTVVVRIQ

Enzyme Prediction     

No EC number prediction in MGYG000003137_04758.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	117	276	9.4e-52	0.8983050847457628

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
TIGR02073	PBP_1c	0.0	79	745	1	727	penicillin-binding protein 1C. This subfamily of the penicillin binding proteins includes the member from E. coli designated penicillin-binding protein 1C. Members have both transglycosylase and transpeptidase domains and are involved in forming cross-links in the late stages of peptidoglycan biosynthesis. All members of this subfamily are presumed to have the same basic function. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG4953	PbpC	0.0	54	745	6	732	Membrane carboxypeptidase/penicillin-binding protein PbpC [Cell wall/membrane/envelope biogenesis].
PRK11240	PRK11240	1.06e-167	47	633	4	580	penicillin-binding protein 1C; Provisional
COG0744	MrcB	2.56e-107	38	638	1	613	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	3.77e-102	122	611	11	520	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ABQ35986.1	0.0	1	745	1	745
SMX58442.1	0.0	36	745	1	706
CAL77680.1	0.0	34	745	27	734
BAM89948.1	0.0	36	745	1	706
SPP95548.1	0.0	14	745	5	731

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3UDF_A	1.62e-35	108	614	36	710	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
4OON_A	6.38e-34	92	592	23	694	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
5FGZ_A	3.76e-31	98	598	143	673	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]
3VMA_A	4.00e-31	98	598	164	694	CrystalStructure of the Full-Length Transglycosylase PBP1b from Escherichia coli [Escherichia coli K-12]
2OQO_A	9.78e-31	101	289	10	197	Crystalstructure of a peptidoglycan glycosyltransferase from a class A PBP: insight into bacterial cell wall synthesis [Aquifex aeolicus VF5],3D3H_A Crystal structure of a complex of the peptidoglycan glycosyltransferase domain from Aquifex aeolicus and neryl moenomycin A [Aquifex aeolicus],3NB7_A Crystal structure of Aquifex Aeolicus Peptidoglycan Glycosyltransferase in complex with Decarboxylated Neryl Moenomycin [Aquifex aeolicus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P76577	4.22e-131	77	623	31	572	Penicillin-binding protein 1C OS=Escherichia coli (strain K12) OX=83333 GN=pbpC PE=1 SV=1
P38050	1.50e-56	89	592	48	569	Penicillin-binding protein 1F OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpF PE=2 SV=2
Q9KUC0	1.92e-44	128	598	196	694	Penicillin-binding protein 1B OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) OX=243277 GN=mrcB PE=3 SV=1
O66874	1.90e-43	89	592	44	639	Penicillin-binding protein 1A OS=Aquifex aeolicus (strain VF5) OX=224324 GN=mrcA PE=1 SV=1
P70997	2.24e-41	89	632	54	621	Penicillin-binding protein 2D OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpG PE=2 SV=3

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999812	0.000191	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
55	77