CAZyme Information: MGYG000003147_01047

Basic Information

• Species: Robinsoniella sp900555455
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Robinsoniella; Robinsoniella sp900555455
• CAZyme ID: MGYG000003147_01047
• CAZy Family: GH84
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2020	MGYG000003147_4|CGC4	223347.14	4.7725

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003147	4940098	MAG	United States	North America

• Gene Location: Start: 233175; End: 239237 Strand: +

Full Sequence      

MKVILYLQARFGQGYLINQADESIKQIGGLRMKRLRKALSFLLIIVMTMTSVQLSGISET
LQVSAAENAAYEIYPTPHDVEYPDGSFTLSDEVNVIFGEGIDQATKDKVDDVLKTAGVTH
VVTDATVQGKTNLLVGIHRSEGAKDAADQFAAEHTAVSDDHFNQTDAYVLTAQDNTIAAV
GKDTNAAFYALSTLKMVLRQITGKQLQNFTVTDFSDTGLRGFIEGYYGLPWSNEDRMNLM
KFGGEIKCNAYVFAPKDDPYHNSKWREMYPEEKLAEIREMAEVGRKSKCEFVWTIHPFMS
DRITESNYDDSLATIKKKFQQLYDAGVRRFGVLADDAGGVSVNLIANLLNDLTDWCKSKN
DVGDILYCPPEYNWAFAGYSWTNLRNANQLVNDDVDFFWTGQSVCGHVTKETVDGFVNGL
TSDAKAGRKPLFWLNWPVNDINKVRMLMGKGEMLNTDVTNLAGVITNPMQQSEPSKVAIF
AVADYAWNIQGFNRDKSWADSFKYVEPDASQALHTMAKHLSDPAPNGHSLVLGESEELAP
LLEEFMTLYQSGATIQEKGSRLVEEFQKIADAADAFERDSQNENLKAQLSPWTDSLRYLA
KAAINYINTAIAYENNDYENVWSYFSEASSYYGLSKQCPVVNIDSVPNVEAGAKRLIPFV
KNMSDVLGPKVNSVVNPNADKTRPFVPLDAELMKSDGINGTYNEYTEGKATDKDDTTFAW
FKTTPNDSFPAGGWLGLDLGREVTLGTIKYLQGGTTTAQSDLISSAVLEYSLDGENYTEL
ESYKDKRDIYFNAEELGIKARYVRVRAKAATGKWLAVREFAVSEGEVLPSTVAYTNVASL
SRRSVTTTADTADLSVNDRDLTLGKNQYLGIMLPRIRNIMEINAAYTQQNKLTMEASVNG
TEWQTVDPGNLTGGLDARYIRLINSSKDPVTFQLSRFTIRSKEIAAPQFRESNIAASAIT
NGDQAVDGDRSTKANFSVSQKEGQYIIYDLGQTIPLKKLKMVQHDSEVDFIRNGKISVSA
DLENWTDILTIGNPDKDPGQVTIDECFPDHEISYYTLSTAEPVNQDTRYIKISVTKDYNA
RWIRFNELEINDNEYIPVENNPSFVTTDVEEKGHRPSYLTDNDVSTTYRSSKKEGEGSLT
YRVSGNTNVTGITILQSPNTISDADVSVRYGNDAWKSIGTLDKSLNVFEDLGQDVSNVFE
IKISWSQTAPELHEMYLVTAEPDPVIPADKSSLIEKYTQALAVDGKYYTKETYAALKNAI
DDAEGVIGYAEASQEEVAAALAALTDAFEKLEDIPADRTELQGAVDKAKELDAKLYTEES
YQAVTQAVAAAEESLGKEGVKQKELDEKLAALEQAVNNLVIKGTLTKIESGELSATAGSQ
EGSGSDGNADQAIDGNETTYWHSNWSGSAAVKPDIPGNVRNEFTIDVGKNRILRKLEYVP
RSNNINGRILGYKLYYSPTENGDDFREVPGGTGTWSDNADKKEVVFNTVNARRIQIRATS
TRGDSGNDKFISAAEFYVYEIIPDEEEQIYHVTFEGGEGTVGEAPESMTAKEGEQITLPD
NTYTREGFTFTGWADGDEIYQPGDSYTVPSYDVIFTAQWQEVIPEIYTISFEGGEGALGD
APVSITGEEGAPITLPDNTFVKAGFAFNGWNDGNGIYQPGDTYHIPGMDTVFTAEWIIDV
PETEVTAYFEGGDGAAGESPEPVTVLAGSSFILPENSYTRDGFVFQGWNDGMNTYQPGSE
YRLIQDITFTAVWGKKPIPTYVVSFHGNGGNTSTSGITVNEGSVISVLPTAARSGYQFLG
WFTAADGGAQFTSSTRVTANITVYAHWKQIVEVPAKVTGLKTSYNKIKSIKITWEKAAKA
KGYHVYRYDSRRNEWKKIKTTTSTSYKNTGLKEGTNYSYRVKAYNQIGSEVKEGSFSDTL
KTASAPAKPSLKVSQTGSKKVKITWKKTSRCDGVEIYMKAENKKKYTKIASKSKSASSLK
KSGLKKGTTYRFKIKRYKRAGSKKIYSSYSSVKKIKINNK

Enzyme Prediction     

No EC number prediction in MGYG000003147_01047.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH84	220	517	1e-97	0.9932203389830508
CBM32	1378	1517	1.6e-17	0.9274193548387096
CBM32	701	821	4.3e-16	0.8870967741935484

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07555	NAGidase	5.51e-121	220	515	1	292	beta-N-acetylglucosaminidase. This family has previously been described as a hyaluronidase. However, more recently it has been shown that this family has beta-N-acetylglucosaminidase activity.
pfam02838	Glyco_hydro_20b	3.75e-21	70	213	1	123	Glycosyl hydrolase family 20, domain 2. This domain has a zincin-like fold.
pfam09479	Flg_new	1.04e-14	1761	1826	1	65	Listeria-Bacteroides repeat domain (List_Bact_rpt). This model describes a conserved core region of about 43 residues, which occurs in at least two families of tandem repeats. These include 78-residue repeats which occur from 2 to 15 times in some proteins of Bacteroides forsythus ATCC 43037, and 70-residue repeats found in families of internalins of Listeria species. Single copies are found in proteins of Fibrobacter succinogenes, Geobacter sulfurreducens, and a few other bacteria.
pfam00754	F5_F8_type_C	2.16e-14	1374	1516	1	127	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
cd00063	FN3	3.72e-10	1834	1919	1	88	Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all animal proteins contain the FN3 repeat; including extracellular and intracellular proteins, membrane spanning cytokine receptors, growth hormone receptors, tyrosine phosphatase receptors, and adhesion molecules. FN3-like domains are also found in bacterial glycosyl hydrolases.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AYE33607.1	0.0	31	1367	4	1466
QAS61771.1	0.0	31	1367	4	1466
QQV04689.1	1.71e-262	58	1360	26	1457
QPS12841.1	1.71e-262	58	1360	26	1457
QMW73892.1	1.71e-262	58	1360	26	1457

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6PWI_A	1.93e-182	71	668	34	625	Structureof CpGH84D [Clostridium perfringens ATCC 13124],6PWI_B Structure of CpGH84D [Clostridium perfringens ATCC 13124]
6PV4_A	6.02e-149	71	676	32	653	Structureof CpGH84A [Clostridium perfringens ATCC 13124],6PV4_B Structure of CpGH84A [Clostridium perfringens ATCC 13124],6PV4_C Structure of CpGH84A [Clostridium perfringens ATCC 13124],6PV4_D Structure of CpGH84A [Clostridium perfringens ATCC 13124]
5MI4_A	8.07e-73	127	601	56	518	BtGH84mutant with covalent modification by MA3 [Bacteroides thetaiotaomicron VPI-5482],5MI5_A BtGH84 mutant with covalent modification by MA3 in complex with PUGNAc [Bacteroides thetaiotaomicron VPI-5482],5MI6_A BtGH84 mutant with covalent modification by MA3 in complex with Thiamet G [Bacteroides thetaiotaomicron VPI-5482],5MI7_A BtGH84 mutant with covalent modification by MA4 in complex with PUGNAc [Bacteroides thetaiotaomicron VPI-5482]
2J4G_A	1.16e-72	127	601	44	506	Bacteroidesthetaiotaomicron GH84 O-GlcNAcase in complex with n-butyl- thiazoline inhibitor [Bacteroides thetaiotaomicron VPI-5482],2J4G_B Bacteroides thetaiotaomicron GH84 O-GlcNAcase in complex with n-butyl- thiazoline inhibitor [Bacteroides thetaiotaomicron VPI-5482]
2CHO_A	1.18e-72	127	601	45	507	Bacteroidesthetaiotaomicron hexosaminidase with O-GlcNAcase activity [Bacteroides thetaiotaomicron VPI-5482],2CHO_B Bacteroides thetaiotaomicron hexosaminidase with O-GlcNAcase activity [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P26831	1.36e-240	33	1360	3	1491	Hyaluronoglucosaminidase OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagH PE=1 SV=2
Q89ZI2	9.77e-72	127	601	66	528	O-GlcNAcase BT_4395 OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_4395 PE=1 SV=1
Q8XL08	1.74e-59	75	805	48	741	O-GlcNAcase NagJ OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagJ PE=1 SV=1
Q0TR53	9.61e-59	75	805	48	741	O-GlcNAcase NagJ OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=nagJ PE=1 SV=1
Q8VIJ5	3.17e-27	219	501	61	348	Protein O-GlcNAcase OS=Rattus norvegicus OX=10116 GN=Oga PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.707230	0.239684	0.051136	0.000763	0.000304	0.000871

TMHMM  Annotations     

start	end
38	57