CAZyme Information: MGYG000003147_02312

Basic Information

• Species: Robinsoniella sp900555455
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Robinsoniella; Robinsoniella sp900555455
• CAZyme ID: MGYG000003147_02312
• CAZy Family: GT2
• CAZyme Description: Cellulose synthase catalytic subunit [UDP-forming]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
627	MGYG000003147_14|CGC4	71609.46	8.832

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003147	4940098	MAG	United States	North America

• Gene Location: Start: 59382; End: 61265 Strand: +

Full Sequence      

MGFLYFLAAVGGSILISNLVKRNRCSTGILVRWNILITSVYIIWRVTVIPTASILELFLG
VLLLGGELLGIAQFLLSQLLLSRKYRLKKKTLADFSGHIPTVDIFICTYNEGEDLVETTV
LAALNLDYPRDKLNIYICDDGQRRYMKRLAARCGVNYMTRGSNEGAKAGNLNNALRHSTG
ELFAVLDADMICSKNFLKHTVGYFSDEDTAFVQTPQVYYNKDMYQRNLGREIPNEQDFFM
REIQESRAAVGAVLHVGTNAVFRRKSVMEIGMYPTNTITEDMAVGMLLQAAGYQSVFINE
VLVLGLTASTYTDLVIQRDRWCRGNIQTSREYHPLRMKGLNRWQKLAYLGGDIYWYTGFS
KMIFTFCPIWFLLTTKLAMQADLVGILTFFVPFFIGQMTSFYAMISNTRSLKWAHYYEIA
MAPHMCLSVLREFFSASLNFAVTPKEMRDDRSHFQAQVILPHMVIAAITLFSWITGAIFL
HLGYIALIPFLVNLVWSIYNFMGVVICIRVAYHIGNIPRSNYATVKSGHKITVETETESY
PGEVMSFLESGMYIHVQAPLYFTKVRIRMQYQELDVTLDGTLKMSGNQGLFKYDWLNLNQ
KRAIVGIYVEHLQPSFNVEKKMHYLES

Enzyme Prediction     

No EC number prediction in MGYG000003147_02312.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT2	103	270	7.7e-31	0.9882352941176471

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06421	CESA_CelA_like	4.66e-102	100	328	1	233	CESA_CelA_like are involved in the elongation of the glucan chain of cellulose. Family of proteins related to Agrobacterium tumefaciens CelA and Gluconacetobacter xylinus BscA. These proteins are involved in the elongation of the glucan chain of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues. They are putative catalytic subunit of cellulose synthase, which is a glycosyltransferase using UDP-glucose as the substrate. The catalytic subunit is an integral membrane protein with 6 transmembrane segments and it is postulated that the protein is anchored in the membrane at the N-terminal end.
PRK11498	bcsA	2.43e-73	100	500	260	665	cellulose synthase catalytic subunit; Provisional
COG1215	BcsA	4.74e-50	83	507	37	430	Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1,6-N-acetylglucosamine synthase [Cell motility].
cd06437	CESA_CaSu_A2	2.34e-32	100	324	1	231	Cellulose synthase catalytic subunit A2 (CESA2) is a catalytic subunit or a catalytic subunit substitute of the cellulose synthase complex. Cellulose synthase (CESA) catalyzes the polymerization reaction of cellulose using UDP-glucose as the substrate. Cellulose is an aggregate of unbranched polymers of beta-1,4-linked glucose residues, which is an abundant polysaccharide produced by plants and in varying degrees by several other organisms including algae, bacteria, fungi, and even some animals. Genomes from higher plants harbor multiple CESA genes. There are ten in Arabidopsis. At least three different CESA proteins are required to form a functional complex. In Arabidopsis, CESA1, 3 and 6 and CESA4, 7 and 8, are required for cellulose biosynthesis during primary and secondary cell wall formation. CESA2 is very closely related to CESA6 and is viewed as a prime substitute for CESA6. They functionally compensate each other. The cesa2 and cesa6 double mutant plants were significantly smaller, while the single mutant plants were almost normal.
cd06435	CESA_NdvC_like	1.85e-31	109	332	7	235	NdvC_like proteins in this family are putative bacterial beta-(1,6)-glucosyltransferase. NdvC_like proteins in this family are putative bacterial beta-(1,6)-glucosyltransferase. Bradyrhizobium japonicum synthesizes periplasmic cyclic beta-(1,3),beta-(1,6)-D-glucans during growth under hypoosmotic conditions. Two genes (ndvB, ndvC) are involved in the beta-(1, 3), beta-(1,6)-glucan synthesis. The ndvC mutant strain resulted in synthesis of altered cyclic beta-glucans composed almost entirely of beta-(1, 3)-glycosyl linkages. The periplasmic cyclic beta-(1,3),beta-(1,6)-D-glucans function for osmoregulation. The ndvC mutation also affects the ability of the bacteria to establish a successful symbiotic interaction with host plant. Thus, the beta-glucans may function as suppressors of a host defense response.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QPJ85522.1	2.78e-183	4	625	2	642
QPJ85521.1	1.68e-179	34	626	36	647
CED93608.1	8.79e-179	13	625	9	624
CDM68038.1	4.49e-154	21	616	24	628
QJA08755.1	1.07e-152	29	432	26	431

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7LBY_A	4.68e-57	100	556	273	733	ChainA, Cellulose synthase catalytic subunit [UDP-forming] [Escherichia coli K-12]
5EJ1_A	2.48e-51	99	446	127	497	ChainA, Putative cellulose synthase [Cereibacter sphaeroides 2.4.1]
4HG6_A	4.98e-51	99	446	139	509	ChainA, Cellulose Synthase Subunit A [Cereibacter sphaeroides]
4P00_A	5.02e-51	99	446	140	510	ChainA, Cellulose Synthase A subunit [Cereibacter sphaeroides 2.4.1],4P02_A Chain A, Cellulose Synthase subunit A [Cereibacter sphaeroides 2.4.1],5EIY_A Chain A, Putative cellulose synthase [Cereibacter sphaeroides 2.4.1],5EJZ_A Chain A, Putative cellulose synthase [Cereibacter sphaeroides 2.4.1]
6YV7_B	9.86e-11	100	296	42	237	MannosyltransferasePcManGT from Pyrobaculum calidifontis [Pyrobaculum calidifontis JCM 11548],6YV8_B Mannosyltransferase PcManGT from Pyrobaculum calidifontis in complex with GDP and Mn2+ [Pyrobaculum calidifontis JCM 11548],6YV9_A Mannosyltransferase PcManGT from Pyrobaculum calidifontis in complex with GDP-Man and Mn2+ [Pyrobaculum calidifontis JCM 11548]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9WX61	3.44e-63	21	605	69	665	Cellulose synthase 1 catalytic subunit [UDP-forming] OS=Komagataeibacter xylinus OX=28448 GN=bcsAI PE=3 SV=1
Q9RBJ2	1.80e-59	21	510	66	563	Putative cellulose synthase 2 OS=Komagataeibacter xylinus OX=28448 GN=bcsABII-A PE=3 SV=1
Q9WX75	1.80e-59	21	510	66	563	Putative cellulose synthase 3 OS=Komagataeibacter xylinus OX=28448 GN=bcsABII-B PE=3 SV=1
Q59167	3.36e-59	21	510	67	564	Cellulose synthase 2 OS=Komagataeibacter hansenii OX=436 GN=acsAII PE=3 SV=1
P19449	4.25e-59	36	605	84	674	Cellulose synthase catalytic subunit [UDP-forming] OS=Komagataeibacter xylinus OX=28448 GN=bcsA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999971	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
4	20
33	55
59	81
351	373
383	405
458	480
485	507