CAZyme Information: MGYG000003147_02970

Basic Information

• Species: Robinsoniella sp900555455
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Robinsoniella; Robinsoniella sp900555455
• CAZyme ID: MGYG000003147_02970
• CAZy Family: CBM67
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1832	MGYG000003147_22|CGC1	201271.93	4.7873

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003147	4940098	MAG	United States	North America

• Gene Location: Start: 20477; End: 25975 Strand: -

Full Sequence      

MSNGFFKKRKQGLALVLAVVMSVVSLETPVFAAEAQDMVSEAEISSIAADTQDTAIYDLK
TDDLVNPVGIDTANPVFSWKMKSTTMGQCQTAYQIIVAKDMEFADVVWDSQKQMSDKSVG
IKYAGNPLEASVTYYWKTSVWDRDGNEIQSYVASFEMGLLGNEGWSQSQWIQVGNSTELE
KVHYSVEADITCLTDAVSLLFNAADTSNFYMFQLNTKDQPGKVIFKPHTWKDGNFATYSK
HNKDVTAYLGSAEEFKSNSAHVKIDVTEKEIKTYMNEQLIDTFSIGELSDQNSKGILPRI
AQLGFRADLEENGTVDNYRLVDYTDNSSGDIVYDYNFDNENPFLSGAVEDGKFVTKGVGI
LLPPQGISTFRKEVTPGNHLVSAKLYTAGLGVYDVFINGERVGTRQDDGAVVYDELKPGY
THHSKRTIYHTYDVTEMVNGAETSVISAHVTSGWWSGQAAGFYGKEEAFRAQLLLTYEDG
STRVIGTDRSWKTALQGPILYGDIYNGETYDANADLSFRQTGYDDSKWTYADLNKEFNGI
ICPQDGPSVRVRNDLELTAQSAVVYRGVVDENENQFGRINITGNYAPVSGFVLKAGETAV
FDMGQNFAGWDEIQAEGKKGTILTMRHSEMLNDSSGLKSRGNDGAQGSIYTANLRSAKAA
GRYIMNGEGIESYHSTSSFYGFRYVEVTTTQDVMIHGMKGIVVSSVADDTGSIATSDEDV
NQLISNILWGQYSNYLSVPTDCPQRDERKGWTADTQVFSSAAAYNADSKGFLRKYMDDMN
DSQVTEGVYNGAYPDTAPYNGYGEIGQLGWGDAGIIIPYNLYKMYGDATVIEENYRNMQE
FMDIFMASTNKMGGGHNHGDWLAYESNDDEVQNLFGIAYYAWDAAMMSEMASVLGKTEDA
ERYQALYEEEKAFFQDMFVQEDGSLKRTEQTACLMALKMDLLPDENSKAVVKQALLDNIK
RNGNKLQTGFLGTAIIMQTLSDIGATDVAYQLLLQHGNPSWLYSVDQGATTVWERWNSYT
IEDGFGPVSMNSFNHYAYGAVAEWMYGYMAGIMYDTQNPGFKHIILQPSPDQSIQKVDCS
YDSAYGRIVSNWSYQDAQFNYDAVIPANTTATISIPVEDGETVTVNGKSYADVTAETDGL
SYIETKDNKAVFEAVSGSYHFSTGVVEYCNITLKNADTSIPCLISVDGSEMQTMPSGIKV
EKGKSITIKAAPVNDVDYACMGWSGDESAEGTQITVTPQNNMTLVAEFAWIGSENLAQQH
PVTSNETGWNIDAWSHANLVDGILTSESGSLGYTTMQGQSPDVDYWVEIDLGEDMDFNRI
QLYPRSDTRSINGGAPNFPKDFSFEVRKENETQYDTIVSNTEYEAAVGKPSVFTFESVNA
RYVRLHVTKLGDPAAADRNYYFLQLAEMGVYNREIIPVIDKTTLEKVIGDAKTYDGKQAD
YTESSWNDFQDALEEAQRIFSDDTADQETVDIVAEALKQAMKDLKEADRVPVVDSVKVSP
GSTVMERGYAQKFTASVMGKNEPQQTVTWSVHGNYSGATIISNDGVLTVGMDETATLLTV
RAASAIDPDKFGTAAVTLKAVKPSDVKVNRISVTASSDRIFIKDKTAVRAVVLPENATNK
NLNWTSSDTKVATVDSQGRVTAKKDGTVRITATAADGTNVSGSCSVKVVKPRVKLNASSI
KLQLKKSTKALKASGLLSGDKIKSWTSKNKKIATVTKSGKITAKKAGNTSITVTTVKGAK
AICRIKVVKSAVKTNKITADKKKVTLKKGRSYQLKITRSPITATDKITYTSSADRFVTVN
KKGKIAAKKKGKAVITVRTSNGKSTKVKVEVV

Enzyme Prediction     

EC	3.2.1.40

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH78	596	1114	1.1e-156	0.9841269841269841
CBM67	363	532	5.1e-25	0.875

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam17389	Bac_rhamnosid6H	9.41e-126	708	1049	1	340	Bacterial alpha-L-rhamnosidase 6 hairpin glycosidase domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam08531	Bac_rhamnosid_N	4.46e-46	382	553	5	172	Alpha-L-rhamnosidase N-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. This domain is probably involved in substrate recognition.
pfam05592	Bac_rhamnosid	9.03e-26	598	688	7	85	Bacterial alpha-L-rhamnosidase concanavalin-like domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
COG5492	YjdB	4.00e-18	1583	1735	176	321	Uncharacterized conserved protein YjdB, contains Ig-like domain [General function prediction only].
pfam17390	Bac_rhamnosid_C	6.49e-16	1059	1128	7	76	Bacterial alpha-L-rhamnosidase C-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCA51920.1	1.08e-279	55	1161	20	1145
ADD61991.1	4.23e-279	55	1161	20	1145
QUT40529.1	4.23e-279	55	1161	20	1145
QQA09206.1	6.46e-278	55	1161	20	1145
ALJ43908.1	1.79e-277	55	1161	20	1145

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3W5M_A	6.96e-149	67	1162	17	1029	CrystalStructure of Streptomyces avermitilis alpha-L-rhamnosidase [Streptomyces avermitilis MA-4680 = NBRC 14893],3W5N_A Crystal Structure of Streptomyces avermitilis alpha-L-rhamnosidase complexed with L-rhamnose [Streptomyces avermitilis MA-4680 = NBRC 14893]
6I60_A	3.32e-108	57	1161	33	939	Structureof alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12],6I60_B Structure of alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12]
6GSZ_A	1.15e-104	370	1125	134	856	Crystalstructure of native alfa-L-rhamnosidase from Aspergillus terreus [Aspergillus terreus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q82PP4	2.75e-148	67	1162	17	1029	Alpha-L-rhamnosidase OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=SAVERM_828 PE=1 SV=1
T2KNB2	1.40e-134	39	1161	23	920	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22090 PE=1 SV=2
P9WF03	1.27e-112	346	1129	152	892	Alpha-L-rhamnosidase OS=Alteromonas sp. (strain LOR) OX=1537994 GN=LOR_34 PE=1 SV=1
T2KPL4	8.21e-84	66	1141	42	932	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22170 PE=2 SV=1
P33747	3.42e-13	1586	1732	35	184	Uncharacterized protein CA_P0160 OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=CA_P0160 PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000319	0.998862	0.000197	0.000220	0.000188	0.000160

TMHMM  Annotations     

start	end
13	35