CAZyme Information: MGYG000003147_03761

Basic Information

• Species: Robinsoniella sp900555455
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Robinsoniella; Robinsoniella sp900555455
• CAZyme ID: MGYG000003147_03761
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
838	MGYG000003147_38|CGC1	98200.06	4.9272

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003147	4940098	MAG	United States	North America

• Gene Location: Start: 9226; End: 11742 Strand: +

Full Sequence      

MIRLELNHGWKMRPLGEIFQEAVVPGSVYTDLLRNGNMEDPFFKDNEIRAKKLMNKDYEY
VCRFVFEPEAFEDCERILLHFDGLDTLADIYVNSQKAGAAFSMHRTWEYEVQDMLKPGEN
ELMVVFHSPLKFLAEAYQRYGNIGNEDTVEGFMHLRKAHYMFGWDWGACLPDAGIFRPVS
LIGIHHARIDNVRIHQEHGAGQVLLTLNVEAEINEKNDNFQNTNRTADKNLVRVEPFHTE
PSHTEGLYMKVLITDPDGRILCEETGTDTFTISQPRLWWPNGIGEQPLYDIKVLLYHKDE
LLDIWQKRIGLRTMTVRREKDQWGESFAHEINGRTIFAMGADYIPEDHLIGRTTRQRTYR
LLEQCRLANFNSVRIWGGGYYPEDWFFDACDEMGLIVWEDFMFACSVYELTPEFEDNIRE
EFITNIKRIRHHASLGLWCGNNEMEMFVEQRCWVTKPSEVRDYLFMYEKLIPEVLKEHDP
DTFYWPASPSSGGSFDKPNDPDRGDVHYWEVWHGNKPFSEYRKYYFRYISEFGFQSIPSL
KTMEEITDDPKDLNLFSYVCEKHERNNGGNGKIVKYMQQMYLYPTSFSTLIYASQLLQAD
GIRYGVEHFRRNRGRCMGAVFWQLNDCWPVISWSAIDSCGRWKALMYYAKRFFAPCMISC
EEESWMTQEADMNRQHFHFDKSIRLNVANETLSERTIKVRWALRNSDASVIRLMEDTIQV
KAMSAVWLDKVELPEIDYMNEYVSYEAEENGVVISEGTVIFTYPKYFRYRDPGLALRIEG
NEIVVTADAYAKSVEILNEEEDLVLSDNYFDMNAGERRVTVISGSTSGLRVRSVYDIH

Enzyme Prediction     

No EC number prediction in MGYG000003147_03761.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	4	696	1.9e-99	0.6675531914893617

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	5.78e-89	6	811	15	799	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	3.76e-17	29	443	90	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam17753	Ig_mannosidase	1.99e-12	771	836	1	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
PRK10150	PRK10150	2.28e-10	9	480	33	441	beta-D-glucuronidase; Provisional
pfam00703	Glyco_hydro_2	1.18e-09	187	312	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ANU78706.2	0.0	10	837	40	848
ASU31771.1	0.0	10	837	40	848
QQQ95478.1	0.0	12	837	1	807
QJU17896.1	0.0	12	837	1	807
QBE96379.1	0.0	2	837	26	840

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VJX_A	5.21e-140	6	823	11	817	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	5.49e-140	6	823	13	819	Structureof a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	5.64e-140	6	823	13	819	ChainA, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	1.48e-139	6	823	11	817	Structureof the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]
2VQU_A	6.04e-138	6	823	11	817	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQU_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A1D911	5.53e-108	6	828	9	834	Beta-mannosidase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=mndB PE=3 SV=1
Q4WAH4	5.53e-108	6	828	9	834	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1
Q0CCA0	1.44e-107	6	824	9	828	Beta-mannosidase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndB PE=3 SV=2
B0YBU9	2.10e-107	6	828	9	834	Beta-mannosidase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=mndB PE=3 SV=1
Q5B7W2	3.90e-107	6	824	9	828	Beta-mannosidase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mndB PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000065	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003147_03761.