CAZyme Information: MGYG000003152_02159

Basic Information

• Species: Prevotella denticola
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella; Prevotella denticola
• CAZyme ID: MGYG000003152_02159
• CAZy Family: GH85
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1061	MGYG000003152_19|CGC1	117039.5	6.6609

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003152	2774939	MAG	United States	North America

• Gene Location: Start: 15790; End: 18975 Strand: -

Full Sequence      

MKKKSTLAALLLTALLSGSPASVMAQNYNFGQLNWKKMVDLFATALQHGKNFPTDEEIAT
EMGMTTTDLSFIKSHVQRRDILDQKGRLIKNTYADRRVWMNLPTGSGSGGDAGYPTGVWH
NDVFSLWNYTALWGSWNHSVAQIPGAWTDAAHKNGCDILGGTIFFDGASSAGAYNDWITY
AGATTSDPKLAYDNYMYVKPLIHMLMYFGMDGVNINWEYKTGTVGNYKGFHKALYKYAKQ
VGFDGFHLGLYGSSSQLTTGEAPNWYADSEGQISDLMLNYNGEGGAETSVQNAKRANSKL
GAKGLWQGFWIVRFDQNWEDMTYDDARELNICLWGEHKDSRFWSYNSGSSTMEQQDHYQQ
FLERTFSGGNRNPLNKEGLSYSNAKMEWAGDTPPMSNWKGFADMVPERSTVKGSFPFATN
FCLGNGDRYNYRGKKVSGAWYNMSAQDIVPTYRWLVLKANEKVSDAAQISKDVTPSFTHE
DAFTGGTSLRLKATGSTASDIVLYRTDLTTNGAKPYALVATKKNGEKNGQLKLILFTGGQ
WKAYDIPQNGGNSWKEHRISLDGLAHGSKVEYVGLRVENAENGFDAYVGELQLNDGNTAK
PDEVQNVDVTTTSTLVENGTTTVDLKMAWGVNHVANEYGVVYNKEANIDHFEVVYRASDA
NDANVVEVGRTSQWAAFIPALDITGAKKPQVAVVAVSTDLKTVTKPEWHDIKTSSEAGAK
DPFGSYGQSFLDTNAEGYNNAVRLRGVERFTVKGTPDGDYKYELPYADYLKDNSPNGVKN
SARFLNYHHADKTLKVKQGETYEFTLKGFDAQVVTTGTKDDCRYCFVGGWMDFDGSGTFN
YGKGVVEQPFWKDNGFYSYADGTSYANDPDKDQSTYPLDNNTKDGTEAYGERVFRAGTLR
KGNPCLVKGDGLKGKITIPADAHVGKSRLRIVYSDAWFAGAFGPGSKTNKGYTLDIDVDI
VGDNQPGRTYVDKHDVGNPDNWTIVTAVDKVANVTGVPSVKVVNGRLVFENTSKAEIYTV
DGRLVQSIVAPVTAELHTANKTVLIVKLHNNKTVKSVKVVL

Enzyme Prediction     

No EC number prediction in MGYG000003152_02159.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH85	118	429	7.8e-36	0.946031746031746

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG4724	COG4724	3.89e-26	124	595	104	551	Endo-beta-N-acetylglucosaminidase D [Carbohydrate transport and metabolism].
cd06547	GH85_ENGase	3.25e-24	123	456	26	335	Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the protein and N-acetylglucosamine 2 forms the reducing end of the released glycan. ENGase is a key enzyme in the processing of free oligosaccharides in the cytosol of eukaryotes. Oligosaccharides formed in the lumen of the endoplasmic reticulum are transported into the cytosol where they are catabolized by cytosolic ENGases and other enzymes, possibly to maximize the reutilization of the component sugars. ENGases have an eight-stranded alpha/beta barrel topology and are classified as a family 85 glycosyl hydrolase (GH85) domain. The GH85 ENGases are sequence-similar to the family 18 glycosyl hydrolases, also known as GH18 chitinases. An ENGase-like protein is also found in bacteria and is included in this alignment model.
pfam03644	Glyco_hydro_85	2.34e-14	123	426	9	291	Glycosyl hydrolase family 85. Family of endo-beta-N-acetylglucosaminidases. These enzymes work on a broad spectrum of substrates.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUB89911.1	0.0	1	1061	1	1061
AEA21133.1	0.0	1	1061	1	1061
QUB89396.1	0.0	1	1061	1	1061
AXV50373.1	0.0	1	1061	1	1061
QUI93002.1	0.0	1	1061	1	1061

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000281	0.998957	0.000209	0.000183	0.000178	0.000159

TMHMM  Annotations     

start	end
7	29