CAZyme Information: MGYG000003154_01657

Basic Information

• Species: UMGS1490 sp900548185
• Lineage: Bacteria; Verrucomicrobiota; Verrucomicrobiae; Opitutales; CAG-312; UMGS1490; UMGS1490 sp900548185
• CAZyme ID: MGYG000003154_01657
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1228	MGYG000003154_41|CGC1	137071.03	7.1559

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003154	2438738	MAG	United States	North America

• Gene Location: Start: 11778; End: 15464 Strand: -

Full Sequence      

MCLITAAAAAVCAAAGAVKVPAGFAKGQWRTAGDIKMKLSASGVTVQDGLLFNDGQYADA
EYSYRMRAPEGAKEVQAWVSFRIKDRWNRYTVGLRGGNNNILYMARYADDANARFLGFAP
LEFQPKVGEWYDLRVVTVGKVIHVYINGEDKPRLVIEDDTALWDKGSIGVGESYLPAEFS
KAEVRLLDAGEAAAIAKVKSPKQAYEPWKPSADKAKKRAEDRAAYKPVEVSDLSAPRTEV
SLDGKWLFMPTHELEKDAQPVGLDADDSAWHTMNVPDMWVPPLAWLHGETGFPYLQEPAR
GKTAADKLRISEIKRVLGFTFDWDKTKAAWYRNYLYLPSDISGKRFELDFDAIATISKIF
VNGTQVGSHIGMFGDIKCDITKYVKPGKNVIAVEAVRELPKKSTNKTFEVAVTVEITEDM
VNSLPKAFYNFSPAGIWQPAKLVVTNPVSIRDVFVKPALDSAVFEVEIANDGKAPRTVDV
SYTVAPKRERSILYSKKNDQSVTVNPGETRKVEIKTPKLEPRLWTPSEPNLYSVSVRLSE
NGKAVDSVSETFGFRTFGVEGNRVMLNGHPYWLRGANHYPHAARANDRELARKFLSLAKE
NNVNVGRLHVGPVTEAWADAADEVGFIFSVEGIWPWLMLKKGEIPAENLLEAWRNDWASI
VKKYRNHPGIGYWTVNNEMKFSIFDKGDKEMLKKKWAIVDAMIRKMREIDPTRPIVADSG
YVRKEGSYQDYLDVVKANNFDDGDFDDIHRYHGWYNESFMHFMNGNFARNQSLPDRPFIS
QEMSTGYCNEDDGLPSRVYLFNHYTPQALIGDYAFEHNDPNYFLERQSFMTKELGEMFRR
MNRDNMAGVLHFGFVTWFKDSHFKDSIEPRLGVKNLKKFLSPVLVSLELFGRHFYSGEEI
ARDLYVVNDSEDFKAIPPSVLTWQVVESGGNVLASGKENIGAVEYYANEKVPLKIKLPAV
KGRADAKIVLKLEADGKLLSENDYDVVIASRDWARDAGSAKSASVALFDPSGEYAPLLSG
FAVKKMESASQLSGAKVAVIADLGAFMRTKDAQAKLEKFVKDGGGLLLLNPGRAAMDWKG
GALASPEKVRGRIQIRGEGGELSAYREQRGEIVVPKIPESAAFDGLKPMDLAWFADEGSR
DVPLASTAIFQIDRKNPNVRELAEEIQIHAYLNKPSDVLQYSGSPLLEIDDGKGRVIVSE
MRLKAAKSDPVAQRLLTNLVSKLASGRE

Enzyme Prediction     

No EC number prediction in MGYG000003154_01657.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	233	975	1e-88	0.726063829787234

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	6.49e-35	319	990	55	667	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	4.00e-29	329	755	67	476	beta-D-glucuronidase; Provisional
PRK10340	ebgA	2.35e-22	331	713	113	470	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam00703	Glyco_hydro_2	1.17e-16	448	555	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
pfam02836	Glyco_hydro_2_C	3.96e-12	557	782	1	209	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
SDT89154.1	0.0	27	1224	23	1189
AQQ70440.1	2.23e-272	27	1220	50	1231
QIU96993.1	9.49e-266	51	1227	62	1232
SDS47501.1	2.89e-263	39	1223	47	1203
QDO68995.1	8.37e-263	23	1223	20	1227

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6B6L_A	1.27e-26	234	717	2	436	Thecrystal structure of glycosyl hydrolase family 2 (GH2) member from Bacteroides cellulosilyticus DSM 14838 [Bacteroides cellulosilyticus DSM 14838],6B6L_B The crystal structure of glycosyl hydrolase family 2 (GH2) member from Bacteroides cellulosilyticus DSM 14838 [Bacteroides cellulosilyticus DSM 14838]
7RSK_A	2.23e-26	234	717	2	436	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7RSK_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838]
6XXW_A	3.99e-24	324	758	69	476	Structureof beta-D-Glucuronidase for Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12]
3GM8_A	4.41e-24	266	738	30	449	ChainA, Glycoside hydrolase family 2, candidate beta-glycosidase [Phocaeicola vulgatus ATCC 8482]
6D8K_A	6.62e-23	328	782	99	502	Bacteroidesmultiple species beta-glucuronidase [Bacteroides ovatus],6D8K_B Bacteroides multiple species beta-glucuronidase [Bacteroides ovatus],6D8K_C Bacteroides multiple species beta-glucuronidase [Bacteroides ovatus],6D8K_D Bacteroides multiple species beta-glucuronidase [Bacteroides ovatus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KPJ7	1.13e-26	237	768	51	517	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
T2KM09	1.67e-22	235	720	44	459	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
P77989	2.33e-21	328	675	57	384	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
P24131	2.83e-16	241	678	48	459	Beta-galactosidase OS=Clostridium acetobutylicum OX=1488 GN=cbgA PE=2 SV=2
A7LXS9	6.17e-16	231	717	36	499	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.518701	0.473149	0.007202	0.000315	0.000266	0.000363

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003154_01657.