CAZyme Information: MGYG000003156_00005

Basic Information

• Species: Eubacterium_R sp900544515
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; Eubacterium_R; Eubacterium_R sp900544515
• CAZyme ID: MGYG000003156_00005
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase BoGH2A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
689	MGYG000003156_1|CGC1	78733.44	6.6573

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003156	1833273	MAG	United States	North America

• Gene Location: Start: 5836; End: 7905 Strand: -

Full Sequence      

MREKRILSDHWQFIYGLSDISEINANTDWKDISVPHTWNNLDGQDGGGDYLRGKGWYKYL
LDVEKDSSKEYWLEFVGVNSVADLYVNGIHIGQHRGGYTLFRFNVTDALVNGNNQILVCA
DNSPFADVIPLTADFTFFGGIYRDVNLIVCNKTHFALDDYGSDGVNVTYHNSPEIIDNAV
LNVGARINGITDNCSVTAEVTVPDTFEKCDDISVTDFNTDYEMSYDKKVIDKTKGNIVNG
RFDSALTIHHPHLWNGRKDPFLYKVVFTLYNGDTEIETIEKYVGIRCFSIDSRKGFFLNG
ASYPLRGVNRHQDRKDMGWAITAKEHDEDFAMIYEIGANAIRLAHYPHHPHFYDLCDRYG
LLVWAEIPFVDRIGGLGISGLPTDKVKNKAVEESFLNNAKLQMTELIKQQGHRPSIFCWS
MSNEVMAEYGKTAQYMMEELDKLVHSLDNQRYSALATNHTKSYKWKADIRGCNIYPGWYG
GKETHFPFQANYHMRANRLKGVAVSEYGAGSNVLHHSEKPSQPKDTVCDFHSEEWQSIVH
ENALKYFMKKRSNKIWGTFVWNMFDFAIDSRNEGAMPGMNNKGLVTYDRKIKKDAFYLYK
SYWSSVPVVHITSSRFSEREERTIDVKVYSNAQEVTLSLNGKKIAAIKDKDNKQSHIFVF
SSVHLKNGDNTITAVADNISDTVNWKYNQ

Enzyme Prediction     

No EC number prediction in MGYG000003156_00005.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	2	642	2.6e-99	0.663563829787234

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	3.17e-53	1	452	9	428	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	7.14e-47	2	610	10	599	beta-D-glucuronidase; Provisional
PRK09525	lacZ	5.85e-30	4	363	52	410	beta-galactosidase.
PRK10340	ebgA	3.37e-29	28	445	70	473	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam02836	Glyco_hydro_2_C	7.54e-24	296	603	8	298	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCI62299.1	4.45e-174	7	685	28	698
QUT74077.1	4.76e-168	1	674	25	651
QJA07774.1	1.40e-161	1	676	1	637
QOG29427.1	7.25e-161	1	676	1	639
QIZ08201.1	2.02e-159	1	676	1	641

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6MVG_A	4.22e-145	1	681	24	650	Crystalstructure of FMN-binding beta-glucuronidase from Ruminococcus gnavus [[Ruminococcus] gnavus],6MVG_B Crystal structure of FMN-binding beta-glucuronidase from Ruminococcus gnavus [[Ruminococcus] gnavus],6MVG_C Crystal structure of FMN-binding beta-glucuronidase from Ruminococcus gnavus [[Ruminococcus] gnavus]
6MVH_A	5.24e-140	1	681	24	650	Crystalstructure of FMN-binding beta-glucuronidase from Roseburia hominis [Roseburia hominis],6MVH_B Crystal structure of FMN-binding beta-glucuronidase from Roseburia hominis [Roseburia hominis],6MVH_C Crystal structure of FMN-binding beta-glucuronidase from Roseburia hominis [Roseburia hominis],6MVH_D Crystal structure of FMN-binding beta-glucuronidase from Roseburia hominis [Roseburia hominis]
7KGZ_A	5.09e-134	1	683	1	619	ChainA, Beta-glucuronidase [Roseburia hominis],7KGZ_B Chain B, Beta-glucuronidase [Roseburia hominis]
3CMG_A	2.34e-133	1	689	3	657	Crystalstructure of putative beta-galactosidase from Bacteroides fragilis [Bacteroides fragilis NCTC 9343]
5Z1A_A	3.41e-133	1	689	22	676	Thecrystal structure of Bacteroides fragilis beta-glucuronidase in complex with uronic isofagomine [Bacteroides fragilis NCTC 9343]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KPJ7	8.07e-88	2	675	51	703	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
A7LXS9	5.64e-43	2	657	42	707	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	1.58e-42	8	650	52	668	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
P77989	5.90e-38	7	451	19	410	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
T2KN75	3.56e-35	11	668	35	663	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000080	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003156_00005.