dbCAN-seq: a database of CAZyme sequence and annotation

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CAZyme Information: MGYG000003162_00751

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Basic Information help

Species

Lineage

Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Ruminococcus_D;

CAZyme ID

MGYG000003162_00751

CAZy Family

GH5

CAZyme Description

hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
678		74269.67	5.0101

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003162	1479112	MAG	United States	North America

Gene Location

Start: 514; End: 2550 Strand: +

Full Sequence Download help

Enzyme Prediction help

EC	3.2.1.8	3.1.1.73

CAZyme Signature Domains help

Family	Start	End	Evalue	family coverage
GH5	82	352	4.9e-86	0.9927536231884058
CBM65	410	526	2.2e-28	0.9824561403508771
CBM65	559	672	1.4e-25	0.9736842105263158

CDD Domains download full data without filtering help

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam00150	Cellulase	1.81e-58	77	352	10	269	Cellulase (glycosyl hydrolase family 5).
COG2730	BglC	6.04e-34	61	367	45	376	Aryl-phospho-beta-D-glucosidase BglC, GH1 family [Carbohydrate transport and metabolism].
pfam18259	CBM65_1	1.12e-26	417	526	6	113	Carbohydrate binding module 65 domain 1. This domain is found in the non-catalytic carbohydrate binding module 65B (CMB65B) present in Eubacterium cellulosolvens. CBMs are present in plant cell wall degrading enzymes and are responsible for targeting, which enhances catalysis. CBM65s display higher affinity for oligosaccharides, such as cellohexaose, and particularly polysaccharides than cellotetraose, which fully occupies the core component of the substrate binding cleft. The concave surface presented by beta-sheet 2 comprises the beta-glucan binding site in CBM65s. C6 of all the backbone glucose moieties makes extensive hydrophobic interactions with the surface tryptophans of CBM65s. Three out of the four surface Trp are highly conserved. The conserved metal ion site typical of CBMs is absent in this CBM65 family.
pfam18259	CBM65_1	7.95e-21	562	673	1	113	Carbohydrate binding module 65 domain 1. This domain is found in the non-catalytic carbohydrate binding module 65B (CMB65B) present in Eubacterium cellulosolvens. CBMs are present in plant cell wall degrading enzymes and are responsible for targeting, which enhances catalysis. CBM65s display higher affinity for oligosaccharides, such as cellohexaose, and particularly polysaccharides than cellotetraose, which fully occupies the core component of the substrate binding cleft. The concave surface presented by beta-sheet 2 comprises the beta-glucan binding site in CBM65s. C6 of all the backbone glucose moieties makes extensive hydrophobic interactions with the surface tryptophans of CBM65s. Three out of the four surface Trp are highly conserved. The conserved metal ion site typical of CBMs is absent in this CBM65 family.

CAZyme Hits help

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AEI34646.1	7.21e-124	37	526	363	844
AWV80935.1	7.21e-124	37	526	363	844
AAK81397.1	7.21e-124	37	526	363	844
ADZ22510.1	7.21e-124	37	526	363	844
ACZ98591.1	2.52e-119	49	675	375	963

PDB Hits download full data without filtering help

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6UI3_A	2.31e-111	49	380	5	335	GH5-4broad specificity endoglucanase from Clostridum cellulovorans [Clostridium cellulovorans]
6GL2_A	1.63e-88	54	381	8	336	Structureof ZgEngAGH5_4 wild type at 1.2 Angstrom resolution [Zobellia galactanivorans]
6GL0_A	5.24e-88	53	381	1	330	Structureof ZgEngAGH5_4 in complex with a cellotriose [Zobellia galactanivorans],6GL0_B Structure of ZgEngAGH5_4 in complex with a cellotriose [Zobellia galactanivorans],6GL0_C Structure of ZgEngAGH5_4 in complex with a cellotriose [Zobellia galactanivorans]
3NDY_A	1.61e-87	53	371	5	328	Thestructure of the catalytic and carbohydrate binding domain of endoglucanase D from Clostridium cellulovorans [Clostridium cellulovorans],3NDY_B The structure of the catalytic and carbohydrate binding domain of endoglucanase D from Clostridium cellulovorans [Clostridium cellulovorans],3NDY_C The structure of the catalytic and carbohydrate binding domain of endoglucanase D from Clostridium cellulovorans [Clostridium cellulovorans],3NDY_D The structure of the catalytic and carbohydrate binding domain of endoglucanase D from Clostridium cellulovorans [Clostridium cellulovorans],3NDZ_A The structure of the catalytic and carbohydrate binding domain of endoglucanase D from Clostridium cellulovorans bound to cellotriose [Clostridium cellulovorans],3NDZ_B The structure of the catalytic and carbohydrate binding domain of endoglucanase D from Clostridium cellulovorans bound to cellotriose [Clostridium cellulovorans],3NDZ_C The structure of the catalytic and carbohydrate binding domain of endoglucanase D from Clostridium cellulovorans bound to cellotriose [Clostridium cellulovorans],3NDZ_D The structure of the catalytic and carbohydrate binding domain of endoglucanase D from Clostridium cellulovorans bound to cellotriose [Clostridium cellulovorans]
6PZ7_A	1.37e-85	52	373	1	326	GH5-4broad specificity endoglucanase from Clostridium acetobutylicum [Clostridium acetobutylicum ATCC 824]

Swiss-Prot Hits download full data without filtering help

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P28623	2.34e-85	53	371	36	359	Endoglucanase D OS=Clostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B) OX=573061 GN=engD PE=1 SV=2
P28621	1.43e-81	53	381	35	371	Endoglucanase B OS=Clostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B) OX=573061 GN=engB PE=3 SV=1
P54937	6.79e-78	59	391	38	385	Endoglucanase A OS=Clostridium longisporum OX=1523 GN=celA PE=1 SV=1
P23661	4.82e-73	59	391	70	406	Endoglucanase B OS=Ruminococcus albus OX=1264 GN=celB PE=3 SV=1
P10477	1.64e-72	55	381	52	382	Cellulase/esterase CelE OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=celE PE=1 SV=2

SignalP and Lipop Annotations help

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000292	0.999034	0.000181	0.000200	0.000156	0.000139

TMHMM Annotations download full data without filtering help

start	end
12	31