CAZyme Information: MGYG000003184_03706

Basic Information

• Species: Mixta calida
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Mixta; Mixta calida
• CAZyme ID: MGYG000003184_03706
• CAZy Family: GH3
• CAZyme Description: Beta-glucosidase BoGH3B

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
793	MGYG000003184_37|CGC1	86274.82	5.3435

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003184	4133670	MAG	United States	North America

• Gene Location: Start: 19308; End: 21689 Strand: -

Full Sequence      

MTAIYQDPQRPVAERVADLLARMTPEEKFAQMHAFWLILSADGNHRERSDLSDSFSGAGQ
LNDLNARLRRGVGQITRPLGTHVVDARDGVRAANHLQKTLMEETRLGIPALFHEECLVGL
LCKDATLFPSPLNYASTWDPELIEQVAAAIGEEARSTGCRQGLAPVLDVSRDVRWGRTEE
TFGEDPWLVGVMATHFVRGLQGEKRDLLATLKHYVGHSFSEGGRNHAPVHLGFCELNDTF
LLPFEMAVKLANAGSVMPAYHDIDNQPSHSDAFLLTTLLRERWGFDGIIVADYGGVSLLH
QHHGVARDAAESAALAFNAGLDIELPKDDCAQHLAQAVERGLITMEKVDEIVARLLTEKF
RLGLFEQPYADENRISLQSENARRLARETATRSITLLENNGALPLKPEQRVAVIGPTADD
PLALLSGYSFPVHLIISDMQDQAAQVTTPLQALREALGDTQVSYARGCHIIEKRMAGAPV
FPGDSAGKPMQQSPVSQDTSLIAEAVALAENSDVVVACVGDLAGLFQSGTVGEGSDTDSL
ALPGVQQQLLAALVATGKPVVVALTGGRPYHLSGLEDRVAALLMAWAPGQEGGYALADVL
TGRAEPTGRLVLSVPKSAGAMPYYYNHKLKSGGTPFAFHFGARYPFGYGKTWTEFTYGPL
SLAQPETPVAQGTIEVALEITNSGQRAGSEVVQLYVRDNVASMVRPVQELKAFQRVMLEP
GETARLTFFLPTDMLNFTSREGERIVEPGEFELQVGASSGDIRQRTVVTLTGETRRLPQQ
WRMLSHCEVARGR

Enzyme Prediction     

EC	3.2.1.37

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	102	326	3.5e-65	0.9861111111111112

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK15098	PRK15098	1.44e-169	16	764	38	759	beta-glucosidase BglX.
COG1472	BglX	8.89e-86	23	470	1	397	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
pfam00933	Glyco_hydro_3	3.93e-78	24	357	1	315	Glycosyl hydrolase family 3 N terminal domain.
PLN03080	PLN03080	4.15e-71	105	756	78	769	Probable beta-xylosidase; Provisional
pfam01915	Glyco_hydro_3_C	7.23e-56	394	649	1	213	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AUY24020.1	0.0	1	793	1	793
QNU44002.1	0.0	1	793	1	793
AIX75030.1	0.0	1	793	1	793
QFS62703.1	0.0	1	793	1	789
AUX92188.1	0.0	1	792	1	792

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5Z87_A	2.52e-134	88	770	103	784	ChainA, EmGH1 [Aurantiacibacter marinus],5Z87_B Chain B, EmGH1 [Aurantiacibacter marinus]
5Z9S_A	5.29e-128	2	759	14	775	Functionaland Structural Characterization of a beta-Glucosidase Involved in Saponin Metabolism from Intestinal Bacteria [Bifidobacterium longum],5Z9S_B Functional and Structural Characterization of a beta-Glucosidase Involved in Saponin Metabolism from Intestinal Bacteria [Bifidobacterium longum]
5XXL_A	3.09e-127	19	763	17	745	Crystalstructure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXL_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXM_A Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482],5XXM_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482]
5XXN_A	1.69e-126	19	763	17	745	CrystalStructure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXN_B Crystal Structure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXO_A Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482],5XXO_B Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482]
5YOT_A	1.05e-125	5	763	7	749	Isoprimeverose-producingenzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40],5YOT_B Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40],5YQS_A Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40],5YQS_B Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P33363	1.28e-117	16	764	38	759	Periplasmic beta-glucosidase OS=Escherichia coli (strain K12) OX=83333 GN=bglX PE=3 SV=2
Q56078	2.52e-117	16	764	38	759	Periplasmic beta-glucosidase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=bglX PE=3 SV=2
T2KMH0	3.51e-116	105	759	62	710	Beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22130 PE=1 SV=1
T2KMH9	6.49e-108	5	764	29	749	Putative beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22230 PE=2 SV=1
A7LXU3	7.33e-81	8	762	30	768	Beta-glucosidase BoGH3B OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02659 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000080	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003184_03706.