CAZyme Information: MGYG000003201_01598

Basic Information

• Species: CAG-873 sp900759825
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Muribaculaceae; CAG-873; CAG-873 sp900759825
• CAZyme ID: MGYG000003201_01598
• CAZy Family: GH97
• CAZyme Description: Retaining alpha-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
620		69877.97	5.1168

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003201	2637500	MAG	United States	North America

• Gene Location: Start: 6278; End: 8140 Strand: -

Full Sequence      

MKLAFPAAALLIAASASAQTLHIASPDNRLEATFAKTGDELTYSIDYDGKPLILPSRAGI
ELDNRVWEMALGKRDLAQPDSWMDLLTVDSVTVAAPVDSVWHPLYGERSAVRDRFNAATL
HMSRKDKSQYRLDVEVRAYDEGIAWRYFLPEHPDAIFHKVVGDLTDYAFPAGTVAWSEQW
AQARFDVGPVDSITEPVERALTLRTPDNRWIALLDADTDDWCLTKFRARPGHPSTLESVM
YSPVDVVTYAATPWKIVMAADTPGELIENNDIVLNLNPPATGDFSWVKPGTIMRCTRLTT
EAAIENIDFCAEHNIPYMLYDWKWYAGPTSHDSDATKVIDAIDMRRVVEYGREKGVGVWL
YVNQHALMKQMDELFPLLHEWGIVGVKSGFVQYASHRWATWLHDMVRKAADNGLMMNIHD
EYRPSGFSRTYPNLLTQEGICGNEEWPDATHDVTLPFTRMLNGAADYTICYFDQRLRNTH
AHQLAASLVFYSPLMTILWYDTPGRYHGEPEIAWFEGLATVWDDTRVLSGAPGESIAMAR
RKGSDWWLGAMANNDGARQQIDLDFLDDGANYMADIYTDSTRDTGTATRVAVGHRKVRKG
QRLAFDLLPRGGVAIKFTRI

Enzyme Prediction     

No EC number prediction in MGYG000003201_01598.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH97	11	617	2.5e-157	0.9904912836767037

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam10566	Glyco_hydro_97	1.46e-59	283	515	1	273	Glycoside hydrolase 97. This domain is the catalytic region of the bacterial glycosyl-hydrolase family 97. This central part of the GH97 family protein sequences represents a typical and complete (beta/alpha)8-barrel or catalytic TIM-barrel type domain. The N- and C-terminal parts of the sequences, mainly consisting of beta-strands, form two additional non-catalytic domains. In all known glycosidases with the (beta-alpha)8-barrel fold, the amino acid residues at the active site are located on the C-termini of the beta-strands.
pfam14508	GH97_N	2.20e-58	25	279	1	235	Glycosyl-hydrolase 97 N-terminal. This N-terminal domain of glycosyl-hydrolase-97 contributes part of the active site pocket. It is also important for contact with the catalytic and C-terminal domains of the whole.
pfam14509	GH97_C	3.25e-33	522	618	1	97	Glycosyl-hydrolase 97 C-terminal, oligomerization. Glycosyl-hydrolase-97 is made up of three tightly linked and highly conserved globular domains. The C-terminal domain is found to be necessary for oligomerization of the whole molecule in order to create the active-site pocket and the Ca++-binding site.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT34121.1	2.90e-305	21	620	25	631
QUU00177.1	5.83e-305	21	620	25	631
QUT92217.1	2.36e-304	21	620	25	631
ALJ62032.1	2.74e-303	21	620	25	631
QDO69864.1	3.89e-303	21	620	25	631

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5E1Q_A	1.77e-56	25	616	20	653	Mutant(D415G) GH97 alpha-galactosidase in complex with Gal-Lac [Bacteroides thetaiotaomicron VPI-5482],5E1Q_B Mutant (D415G) GH97 alpha-galactosidase in complex with Gal-Lac [Bacteroides thetaiotaomicron VPI-5482]
3A24_A	1.35e-55	25	616	6	639	Crystalstructure of BT1871 retaining glycosidase [Bacteroides thetaiotaomicron],3A24_B Crystal structure of BT1871 retaining glycosidase [Bacteroides thetaiotaomicron]
3WFA_A	1.41e-50	23	580	5	664	Catalyticrole of the calcium ion in GH97 inverting glycoside hydrolase [Bacteroides thetaiotaomicron],3WFA_B Catalytic role of the calcium ion in GH97 inverting glycoside hydrolase [Bacteroides thetaiotaomicron]
2JKA_A	1.42e-50	23	580	14	673	Nativestructure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JKA_B Native structure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JKE_A Structure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron in complex with deoxynojirimycin [Bacteroides thetaiotaomicron VPI-5482],2JKE_B Structure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron in complex with deoxynojirimycin [Bacteroides thetaiotaomicron VPI-5482],2JKP_A Structure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron in complex with castanospermine [Bacteroides thetaiotaomicron VPI-5482],2JKP_B Structure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron in complex with castanospermine [Bacteroides thetaiotaomicron VPI-5482]
2D73_A	1.59e-50	23	580	25	684	CrystalStructure Analysis of SusB [Bacteroides thetaiotaomicron VPI-5482],2D73_B Crystal Structure Analysis of SusB [Bacteroides thetaiotaomicron VPI-5482],2ZQ0_A Crystal structure of SusB complexed with acarbose [Bacteroides thetaiotaomicron],2ZQ0_B Crystal structure of SusB complexed with acarbose [Bacteroides thetaiotaomicron]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8A6L0	1.49e-55	2	616	3	660	Retaining alpha-galactosidase OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_1871 PE=1 SV=1
G8JZS4	8.72e-50	23	580	25	684	Glucan 1,4-alpha-glucosidase SusB OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=susB PE=1 SV=1
D7CFN7	1.04e-42	131	614	131	617	Probable retaining alpha-galactosidase OS=Streptomyces bingchenggensis (strain BCW-1) OX=749414 GN=SBI_01652 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000371	0.998842	0.000192	0.000214	0.000187	0.000171

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003201_01598.