CAZyme Information: MGYG000003214_03055

Basic Information

• Species: NSJ-32 sp014384895
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; UBA1390; NSJ-32; NSJ-32 sp014384895
• CAZyme ID: MGYG000003214_03055
• CAZy Family: GH116
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
830		95479.64	4.8985

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003214	5745180	MAG	United States	North America

• Gene Location: Start: 585; End: 3077 Strand: +

Full Sequence      

MRRTEYVGDRTNEISFPLGGIGSGSIGLAGNGRLIDWEIFNRPNKESYNGFSHIAVKAER
QGKLLDARVLNGDVNKGYSGPGTGLENTSMAGFPHFKKCVFYGEFPLAEIQFEDDHFPGK
VTLRAFNPFIPLNDTDSSIPAAFMEVEFQNDGKEELQYSAAFTVRNPDQKGRNQFVKKEL
RFLTTQYAEDHPQFGELCLATDADGEISHQEYWYRGGWSDSLETYWRNFTEQSNLKARRY
EEPGDGDHGTLCVKQMVPAGESVRIRFILSWNYPNNYTYWNPNYKHITWKNYYAVLFSSA
QESAAYGLANWDRLYGETKRFKDELYACSLPDVVIEAISATMSVLKTATVLRLEDGSIYG
WEGLHKNVGSCEGTCTHVWNYAYVLPFLFPKLERSIRNLDYRYNQDESGRMQFRMMLPLE
RPRGTFRACVDGQMGGIVKVYREWKISGDDLWLKEIWPAVKKSLEYAWSEDNEDRWDLDQ
DGVLEGRQHHTLDMELFGPSSWLEGFYLAALKAGEEMARYLGEEDSADEYRELFERGKAW
SEEHLFNGRYYIQQIDLKDASILEPFEGAKEYWNKEVGEIKYQIGEGCEIDQICAQWHAN
IVGLGTIFDKEHVKTALSSLYQQNVKRSMRDFYNPWRLFCLNDEGGAVICEYPEGTKKPA
IPIPYCQETMHGFEYQLAALMISEGMIEEGVEIVAMIRDRYDGKKRNPWNEIECGNNYAR
SMASYSMIPIFSGFIFDMPKGVIGFNPILQKDVFRCIWSLDSAWGNVQIKGEQVLIQILS
GQLTLRELQLPFVTREIHTVRVDGQSVKVKCQGGRIQFDEPMTVQEWIQV

Enzyme Prediction     

No EC number prediction in MGYG000003214_03055.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH116	359	727	2.7e-68	0.9889807162534435

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam04685	DUF608	1.22e-49	371	727	6	362	Glycosyl-hydrolase family 116, catalytic region. This represents a family of archaeal, bacterial and eukaryotic glycosyl hydrolases, that belong to superfamily GH116. The primary catabolic pathway for glucosylceramide is catalysis by the lysosomal enzyme glucocerebrosidase. In higher eukaryotes, glucosylceramide is the precursor of glycosphingolipids, a complex group of ubiquitous membrane lipids. Mutations in the human protein cause motor-neurone defects in hereditary spastic paraplegia. The catalytic nucleophile, identified in UniProtKB:Q97YG8_SULSO, is a glutamine-335, with the likely acid/base at Asp-442 and the aspartates at Asp-406 and Asp-458 residues also playing a role in the catalysis of glucosides and xylosides that are beta-bound to hydrophobic groups. The family is defined as GH116, which presently includes enzymes with beta-glucosidase, EC:3.2.1.21, beta-xylosidase, EC:3.2.1.37, and glucocerebrosidase EC:3.2.1.45 activity.
pfam12215	Glyco_hydr_116N	6.37e-46	29	314	15	309	beta-glucosidase 2, glycosyl-hydrolase family 116 N-term. This domain is found in bacteria, archaea and eukaryotes. This domain is typically between 320 to 354 amino acids in length. This domain is found associated with pfam04685. It is found just after the extreme N-terminus. The N-terminal is thought to be the luminal domain while the C terminal is the cytosolic domain. The catalytic domain of GBA-2 is unknown. The primary catabolic pathway for glucosylceramide is catalysis by the lysosomal enzyme glucocerebrosidase. In higher eukaryotes, glucosylceramide is the precursor of glycosphingolipids, a complex group of ubiquitous membrane lipids. Mutations in the human protein cause motor-neurone defects in hereditary spastic paraplegia. The catalytic nucleophile, identified in UniProtKB:Q97YG8_SULSO, is a glutamine-335 in the downstream family pfam04685.
COG4354	COG4354	7.64e-36	101	735	92	721	Uncharacterized protein, contains GBA2_N and DUF608 domains [Function unknown].
pfam17389	Bac_rhamnosid6H	4.56e-06	441	554	108	217	Bacterial alpha-L-rhamnosidase 6 hairpin glycosidase domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AZN43107.1	0.0	5	820	4	849
QGQ98288.1	0.0	6	826	5	862
QHT59778.1	0.0	5	803	4	827
QGQ98507.1	0.0	1	807	1	839
QHW31016.1	0.0	3	824	2	854

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7DKW_A	1.35e-22	17	709	59	757	ChainA, beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11],7DKW_B Chain B, beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11],7DKX_A Chain A, beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11],7DKY_A Chain A, beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11]
7W2V_A	3.29e-22	17	722	102	817	ChainA, Glucosylceramidase [Thermoanaerobacterium xylanolyticum LX-11]
5FJS_A	5.29e-22	17	709	55	753	Bacterialbeta-glucosidase reveals the structural and functional basis of genetic defects in human glucocerebrosidase 2 (GBA2) [Thermoanaerobacterium xylanolyticum LX-11],5FJS_B Bacterial beta-glucosidase reveals the structural and functional basis of genetic defects in human glucocerebrosidase 2 (GBA2) [Thermoanaerobacterium xylanolyticum LX-11],5O0S_A Crystal structure of txGH116 (beta-glucosidase from Thermoanaerobacterium xylolyticum) in complex with unreacted beta Cyclophellitol Cyclosulfate probe ME711 [Thermoanaerobacterium xylanolyticum LX-11]
5NPF_A	5.38e-22	17	709	58	756	Crystalstructure of txGH116 (beta-glucosidase from Thermoanaerobacterium xylolyticum) in complex with beta Cyclophellitol Cyclosulfate probe ME594 [Thermoanaerobacterium xylanolyticum]
5BVU_A	5.39e-22	17	709	59	757	Crystalstructure of Thermoanaerobacterium xylolyticum GH116 beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11],5BX2_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with 2-deoxy-2-fluoroglucoside [Thermoanaerobacterium xylanolyticum LX-11],5BX3_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with deoxynojirimycin [Thermoanaerobacterium xylanolyticum LX-11],5BX4_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with Glucoimidazole [Thermoanaerobacterium xylanolyticum LX-11],5BX5_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with glucose [Thermoanaerobacterium xylanolyticum LX-11],5NCX_A Crystal structure of Thermoanaerobacterium xylolyticum GH116 beta-glucosidase with an covalent inhibitor [Thermoanaerobacterium xylanolyticum LX-11],5OST_A Beta-glucosidase from Thermoanaerobacterium xylolyticum GH116 in complex with Gluco-1H-imidazole [Thermoanaerobacterium xylanolyticum LX-11]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9HCG7	1.79e-20	17	751	153	892	Non-lysosomal glucosylceramidase OS=Homo sapiens OX=9606 GN=GBA2 PE=1 SV=2
Q69ZF3	2.34e-20	17	751	144	883	Non-lysosomal glucosylceramidase OS=Mus musculus OX=10090 GN=Gba2 PE=1 SV=2
Q5M868	2.77e-19	17	751	144	883	Non-lysosomal glucosylceramidase OS=Rattus norvegicus OX=10116 GN=Gba2 PE=2 SV=2
Q7KT91	1.32e-12	476	692	705	893	Non-lysosomal glucosylceramidase OS=Drosophila melanogaster OX=7227 GN=CG33090 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000053	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003214_03055.