CAZyme Information: MGYG000003214_04965

Basic Information

• Species: NSJ-32 sp014384895
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; UBA1390; NSJ-32; NSJ-32 sp014384895
• CAZyme ID: MGYG000003214_04965
• CAZy Family: GH2
• CAZyme Description: Beta-glucuronidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
574		66137.1	4.9577

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003214	5745180	MAG	United States	North America

• Gene Location: Start: 8950; End: 10674 Strand: +

Full Sequence      

MIPRSEYPRPQFQREESSWQCLNGPWQFAFDFGNSGIDRKLYMPEAEFDREIVVPFCPES
RLSGVEYKDFISAVWYRRTIRIAKAQLENRVLLHFGAVDYHAIVYVNGEKAGEHRGGYVS
FSFDITALLREGENLLVVYAEDDNRHGLQPKGKQSSLFHSHGCDYTRTTGIWQTVWLEFV
PKTYLKSVKTQSDYRNRTVTFTAAMAGQEADATLRACISFEGCVQKEVEVALNAGKTTFT
VELDAVKLWDVGQPNLYDVTYTLYVKGTAVDTVAGYFGVRTVELRDHAIYLNGRPVFQRL
ILDQGFYPDGIYTAPTDEALRRDIELSMELGFNGARLHQKVFEERFLYWADHMGYLVWGE
HASWGLDLHKMENLANFGAEWMEIVERDFNHPAIVGWCPFNETWGGQDPRLLQMMYAITK
AMDTTRPVIDTSGNYHVVTDIYDIHDYEQDVDIFAQRYGAVTREKVYDKFEKQQHYEGQP
YFISEYGGASWNASEGWGYGSAPKTEEEFMQRYAGLTTALMTNEAICAFCYTQLTDVEQE
QNGLYQYDRSKKFSDAVYQAIRDTNRQTAAIERR

Enzyme Prediction     

No EC number prediction in MGYG000003214_04965.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	13	498	6.1e-108	0.5492021276595744

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	5.97e-53	7	509	1	495	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	1.07e-44	21	487	14	510	beta-D-glucuronidase; Provisional
PRK10340	ebgA	1.78e-27	12	492	33	519	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	1.08e-20	20	441	53	499	beta-galactosidase.
pfam02837	Glyco_hydro_2_N	3.57e-20	22	181	4	169	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AUS95984.1	9.52e-285	2	574	3	579
ANU54514.1	4.70e-240	2	573	3	582
QQR31538.1	4.70e-240	2	573	3	582
ASB42259.1	4.70e-240	2	573	3	582
QNF30093.1	1.49e-239	1	573	1	574

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7SF2_A	1.28e-76	6	549	28	565	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_C Chain C, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_D Chain D, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_E Chain E, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_F Chain F, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838]
6U7J_A	1.17e-33	22	487	24	508	UnculturedClostridium sp. Beta-glucuronidase [uncultured Clostridium sp.],6U7J_B Uncultured Clostridium sp. Beta-glucuronidase [uncultured Clostridium sp.],6U7J_C Uncultured Clostridium sp. Beta-glucuronidase [uncultured Clostridium sp.],6U7J_D Uncultured Clostridium sp. Beta-glucuronidase [uncultured Clostridium sp.]
7VQM_A	2.31e-33	22	490	16	502	ChainA, GH2 beta-galacturonate AqGalA [Aquimarina sp.],7VQM_B Chain B, GH2 beta-galacturonate AqGalA [Aquimarina sp.],7VQM_C Chain C, GH2 beta-galacturonate AqGalA [Aquimarina sp.],7VQM_D Chain D, GH2 beta-galacturonate AqGalA [Aquimarina sp.]
4JKM_A	4.11e-33	22	428	18	444	CrystalStructure of Clostridium perfringens beta-glucuronidase [Clostridium perfringens str. 13],4JKM_B Crystal Structure of Clostridium perfringens beta-glucuronidase [Clostridium perfringens str. 13],6CXS_A Crystal Structure of Clostridium perfringens beta-glucuronidase bound with a novel, potent inhibitor 4-(8-(piperazin-1-yl)-1,2,3,4-tetrahydro-[1,2,3]triazino[4',5':4,5]thieno[2,3-c]isoquinolin-5-yl)morpholine [Clostridium perfringens str. 13],6CXS_B Crystal Structure of Clostridium perfringens beta-glucuronidase bound with a novel, potent inhibitor 4-(8-(piperazin-1-yl)-1,2,3,4-tetrahydro-[1,2,3]triazino[4',5':4,5]thieno[2,3-c]isoquinolin-5-yl)morpholine [Clostridium perfringens str. 13]
3K4A_A	1.60e-30	22	487	17	508	Crystalstructure of selenomethionine substituted E. coli beta-glucuronidase [Escherichia coli K-12],3K4A_B Crystal structure of selenomethionine substituted E. coli beta-glucuronidase [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P77989	3.16e-33	22	427	19	411	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
T2KPJ7	2.95e-32	72	490	103	520	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P05804	5.08e-29	22	487	15	506	Beta-glucuronidase OS=Escherichia coli (strain K12) OX=83333 GN=uidA PE=1 SV=2
P26257	4.43e-25	76	560	58	543	Beta-galactosidase OS=Thermoanaerobacterium thermosulfurigenes OX=33950 GN=lacZ PE=1 SV=1
O97524	6.66e-24	7	435	29	486	Beta-glucuronidase OS=Felis catus OX=9685 GN=GUSB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000062	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003214_04965.