CAZyme Information: MGYG000003215_00883

Basic Information

• Species: 1XD42-69 sp014287635
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; 1XD42-69; 1XD42-69 sp014287635
• CAZyme ID: MGYG000003215_00883
• CAZy Family: GH146
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1274	MGYG000003215_104|CGC1	142938.04	4.6874

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003215	2655157	MAG	United States	North America

• Gene Location: Start: 7008; End: 10832 Strand: +

Full Sequence      

MRKHRSIIRLRKATVILLIFTCLLTSLFPEHVTAKKTEQEKNGLVLYYDFSQTESLLTGI
LDMSGNDNTGFIKRVGGESPKEKYKIVNADIYGTTVKALSLPGGADGTYLQLPSGFIDGK
EAVTVSMWIRLAGDASYQRIWDFGSNTEKYIYLLSDGKNAGHEGYAAAITEKGWKNETGV
NKKSDIVKNTWVFTTVVLDKTTMSVYENGKQTGKSVDTGISIRDLGKMTNNCIGYGQFKD
TPTEAEFAEIKIYDKALTAEQIQTMYETFHTQGNGHSNSLREEDQSDKVKTVEEIKVTTQ
EGVSPCLPNNVTVTYQDGSSEKVKAVWPVKIEEGSYAKTGSFTVNGSLTGFDIPVTAEVT
VVEETGESIKQTAENFPLEDITLDKTGANGSILTQNRDREIDYLKQLDKDRMLYNFYKNY
GETDKLKGLEPLAGWDEPDGLLRGHSTGRYMSALALAYSTDGDESLKADLDHVVHELRRM
QEKSSGDPAEFKAKGTNQGVWSKNPGKWGDGYIGAFPPDQFSLLEEYTEYGKIWAPYYTL
HKLLAGFLDAYTYTGNEEALEAAKDLGRWVCRRLGACSREQLAKMWEMYVAGEFGGCNES
LAQLYLYTGDTAYLDGAKLFDNVSFFDNLEKNIDDIAGRHTNQHISQVIGAVKVYEATVK
AGMPQESYYRLADNFWKMVVSRYAYSTGGVGTGEKFTQPYQQADNISGVTNCDTCASYNM
LRLTKALNVYQPDNAEYMDYYERTLYNHMLAAQTPNVTADMSAGTAARVPVGNGVVRTYS
ENYTSFTCCHGTGMESPFRYQEAAYVKTEDTLYVGLYLPSTVTWKEKGIAIKQETKYPSE
NTKFTVSALAGEKSMPVNVKLRVPYWATAGFTVKVNGAVKITDPDVSTYVELTDIKEGDV
IEVYMPWTLHLDQTPDTLNNAMIASVMYGPFVMAAQVNSEDWKTLVLSDDLTKSFTVSKD
EETGFPVLTAQRYSFAPMFSPQYAVKPYDTYFKILYTDDAESRWYEVTVRNDTPKDGTFY
TDADMVKEGDALVITAVPEDGYVVRTLEVNGSQVEMDSDNTYTVQNVRKNIEITGNFRLA
NANKTDEEHLEYSALVTSDFTSNWEDLEGIMRDWEPDRSAAGTGKGWGNWPQQGGSEHYV
QYEWEEAVTMDRFDIYWYDDGGDTAIPASIQIVYKDADGRWKDALMLSDYKDVIEKDTYN
TIRIRPLTTTTVKLILTVQEDKAATGILRWKVSNDNIKDTDQVKPADVNERGWLYRIWNG
LVQNIKNIIFHKKA

Enzyme Prediction     

No EC number prediction in MGYG000003215_00883.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH146	391	934	6e-164	0.9860834990059643

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07944	Glyco_hydro_127	2.87e-128	394	934	12	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG3533	COG3533	8.14e-72	401	952	27	520	Uncharacterized conserved protein, DUF1680 family [Function unknown].
pfam13385	Laminin_G_3	3.90e-17	107	263	4	151	Concanavalin A-like lectin/glucanases superfamily. This domain belongs to the Concanavalin A-like lectin/glucanases superfamily.
pfam07532	Big_4	2.48e-09	292	350	1	58	Bacterial Ig-like domain (group 4). This family consists of bacterial domains with an Ig-like fold. Members of this family are found in a variety of bacterial surface proteins.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BBF45278.1	0.0	23	1233	21	1376
ADF51714.1	6.04e-156	99	967	75	992
AKA69367.1	1.98e-151	38	963	54	1188
AWI04506.1	7.44e-151	38	963	54	1188
AEM69656.1	8.27e-151	97	963	82	993

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6YQH_AAA	3.07e-70	376	949	28	575	ChainAAA, Acetyl-CoA carboxylase, biotin carboxylase [Bacteroides thetaiotaomicron VPI-5482]
5OPJ_A	5.49e-67	376	949	28	575	Beta-L-arabinofuranosidase[Bacteroides thetaiotaomicron]
6EX6_A	1.29e-20	518	915	123	519	TheGH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482],6EX6_B The GH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482]
3WRE_A	4.33e-15	452	952	85	586	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	4.41e-15	452	952	85	586	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	2.37e-14	452	952	85	586	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001621	0.971708	0.025910	0.000276	0.000238	0.000217

TMHMM  Annotations     

start	end
13	32