CAZyme Information: MGYG000003215_00985

Basic Information

• Species: 1XD42-69 sp014287635
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; 1XD42-69; 1XD42-69 sp014287635
• CAZyme ID: MGYG000003215_00985
• CAZy Family: GH39
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1591	MGYG000003215_121|CGC1	173313.76	5.0012

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003215	2655157	MAG	United States	North America

• Gene Location: Start: 12253; End: 17028 Strand: -

Full Sequence      

MKAKVFKRVLSLTLAASLMVIPIPQNVKAEVTVPDPYYEFTFDGGVTDNKVENEGTKTGV
TAEIKGSAEGLGVMEDEVRGNHVLNLPGGSTNGAEEGRLVLPDDMFSDVTDAGFAFSFWI
NIDGNASQYSRIFSGTVNGQNSDAGGGSWNAPEFAFVAGSESASDLGNGQGGYHTSVYLP
DRSAQLKLVWSKQFSRNTWQHVTISVSPTSYDVYLDGASVPITYDRNSNQNTILSTLFAD
GAKVLKQYKYCAIGSSVYQTDKDLKAKLDEFRFYNTALTKEQAKAAYDSYAVRDSVVGSL
RSKVAEAKEKSISFYTKESYEVLSDAIAEGEEGIENPVTEANVNRLISNIDTALAGLVYY
TGITADTAFSLEQLANETAEAKGLLAGGGLSAESEMALEQAITAADAALAAENDQKAVDE
ALIAIRKAVREKSYGSTLHFDTDPKKSKSEVFHGSTGFLYGVSEVGVPSADLIKAIQPKI
LVQKAADGQQHPSGDGYRLTPYLEECDVENIQIYLQDYYLEWPYESNGIDDYNTKVGQIV
TKMLDGKSDEEIAKYSFVIFNEPDGIWYTNKVDQMCNDWLTIYTTIKDICPDAKVAGPNF
SVYNSSAYRKFFAFCKTNNCLPEYITWHELQKDKLTSFASHCKEVKGYVETYYAGSPIEP
VIFVNETVNFDDVGNPGALVNWLSIFDEEDVYASLPYWGLANSMNELAADANKPNGAWWV
YKWYAQMTGNKTPLTLENIGNPSAYGRLYGLTSVDDDAQMVYSLFGGQAGKQTVSIENLT
ATKTFEGADMAHVKIYSTKYTGHHGFADEIPVEYEGNLPLSGDDLIFTIKDAELMDAYFA
IITPSTSDKVTAISAYEKNWEQTYEAEDAELIGNATAYTKANGGDLARSNRAEVGNMNTE
NDGVKFTVDVPQDGRYRMNIYYSSQAPQVDALTLDYVATGGQNRAVGALSTHTLSIDGGS
PQEIVYDSTVKWGYYNYKTVYVDLTAGTHTLQLMYKGESQNGKNVNSMLCALLDKIDLVY
EPDTAKVITVEPEELTASQSGYTLAYSETYSGAGAAVGSGALEFYVSVPRDGYYAVGTKG
NGTAVLKKNRIHYANDAKAESAVTVDRMKLLDLTIGNDNAGMVYLTAGINGMSIEGKDIT
LDLITFTEVTEATEGNSITIEAEDCQLSGQDAGDAYTYLPGSLAVPKVITNAYASNGKAV
EGFRGGQNNTLSFSVTVPEAGDYKLSVTYANDEPAPVMKKQDGGNYVHPYNTDLVERYAQ
IAVNGTTPQTVYFKNTLCWDTYKNTVIDVTLKKGSNTIVVSNDNSYKFSALQDDFTPRFD
KFVIAPAATIQMKQPAYVPDTKGDTPQPTPFDPSQGNQNTGNTTGNTTDTAKDTTKEATL
DAAAFLTDNAGLPTGNKEEAKKSAFAALSARVTKAAKTSNKIKWNKVKDADGYVVFGGKC
NIGKQKNKFEVLTIIKGNGTTSYTHKSLKKGTYYKYIVQAYKVTDGKIQILSTSKTIHSA
TTGGKYGNVKSLTLNKKNVKLKKGGKFTLRVTEKKTTKNLMRHRAVAFESSNAKIATVSR
KGVVKAKKKGSCNIYIYAQNGIYKKIKVTVK

Enzyme Prediction     

No EC number prediction in MGYG000003215_00985.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH39	529	702	2.3e-19	0.3665893271461717

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd04081	CBM35_galactosidase-like	1.07e-19	1159	1324	2	125	Carbohydrate Binding Module family 35 (CBM35); appended mainly to enzymes that bind alpha-D-galactose (CBM35-Gal), including glycoside hydrolase (GH) families GH27 and GH43. This family includes carbohydrate binding module family 35 (CBM35); these are non-catalytic carbohydrate binding domains that are appended mainly to enzymes that bind alpha-D-galactose (CBM35-Gal), including glycoside hydrolase (GH) families GH27 and GH43. Examples of proteins which contain CBM35s belonging to this family includes the CBM35 of an exo-beta-1,3-galactanase from Phanerochaete chrysosporium 9 (Pc1,3Gal43A) which is appended to a GH43 domain, and the CBM35 domain of two bifunctional proteins with beta-L-arabinopyranosidase/alpha-D-galactopyranosidase activities from Fusarium oxysporum 12S, Foap1 and Foap2 (Fo/AP1 and Fo/AP2), that are appended to GH27 domains. CBM35s are unique in that they display conserved specificity through extensive sequence similarity but divergent function through their appended catalytic modules. They are known to bind alpha-D-galactose (Gal), mannan (Man), xylan, glucuronic acid (GlcA), a beta-polymer of mannose, and possibly glucans, forming four subfamilies based on general ligand specificities (galacto, urono, manno, and gluco configurations). Some CBM35s bind their ligands in a calcium-dependent manner. In contrast to most CBMs that are generally rigid proteins, CBM35 undergoes significant conformational change upon ligand binding. GH43 includes beta-xylosidases and beta-xylanases, using aryl-glycosides as substrates, while family GH27 includes alpha-galactosidases, alpha-N-acetylgalactosaminidases, and isomaltodextranases.
cd04083	CBM35_Lmo2446-like	2.90e-12	1200	1305	29	113	Carbohydrate Binding Module 35 (CBM35) domains similar to Lmo2446. This family includes carbohydrate binding module 35 (CBM35) domains that are appended to several carbohydrate binding enzymes. Some CBM35 domains belonging to this family are appended to glycoside hydrolase (GH) family domains, including glycoside hydrolase family 31 (GH31), for example the CBM35 domain of Lmo2446, an uncharacterized protein from Listeria monocytogenes EGD-e. These CBM35s are non-catalytic carbohydrate binding domains that facilitate the strong binding of the GH catalytic modules with their dedicated, insoluble substrates. GH31 has a wide range of hydrolytic activities such as alpha-glucosidase, alpha-xylosidase, 6-alpha-glucosyltransferase, or alpha-1,4-glucan lyase, cleaving a terminal carbohydrate moiety from a substrate that may be a starch or a glycoprotein. Most characterized GH31 enzymes are alpha-glucosidases.
cd04083	CBM35_Lmo2446-like	4.62e-12	863	1018	2	125	Carbohydrate Binding Module 35 (CBM35) domains similar to Lmo2446. This family includes carbohydrate binding module 35 (CBM35) domains that are appended to several carbohydrate binding enzymes. Some CBM35 domains belonging to this family are appended to glycoside hydrolase (GH) family domains, including glycoside hydrolase family 31 (GH31), for example the CBM35 domain of Lmo2446, an uncharacterized protein from Listeria monocytogenes EGD-e. These CBM35s are non-catalytic carbohydrate binding domains that facilitate the strong binding of the GH catalytic modules with their dedicated, insoluble substrates. GH31 has a wide range of hydrolytic activities such as alpha-glucosidase, alpha-xylosidase, 6-alpha-glucosyltransferase, or alpha-1,4-glucan lyase, cleaving a terminal carbohydrate moiety from a substrate that may be a starch or a glycoprotein. Most characterized GH31 enzymes are alpha-glucosidases.
cd04086	CBM35_mannanase-like	3.14e-11	1158	1303	1	107	Carbohydrate Binding Module 35 (CBM35); appended to several carbohydrate binding enzymes, including several glycoside hydrolase (GH) family 26 mannanase domains. This family includes carbohydrate binding module 35 (CBM35) domains that are appended to several carbohydrate binding enzymes, including periplasmic component of ABC-type sugar transport system involved in carbohydrate transport and metabolism, and several glycoside hydrolase (GH) domains, including GH26. These CBM6s are non-catalytic carbohydrate binding domains that facilitate the strong binding of the GH catalytic modules with their dedicated, insoluble substrates. Examples of proteins having CMB35s belonging to this family are mannanase A from Clostridium thermocellum (GH26), Man26B from Paenibacillus sp. BME-14 (GH26), and the multifunctional Cel44C-Man26A from Paenibacillus polymyxa GS01 (which has two GH domains, GH44 and GH26). GH26 mainly includes mannan endo-1,4-beta-mannosidase which hydrolyzes 1,4-beta-D-linkages in mannans, galacto-mannans, glucomannans, and galactoglucomannans, but displays little activity towards other plant cell wall polysaccharides. A few proteins belonging to this family have additional CBM3 domains; these CBM3s are not found in the CBM6-CBM35-CBM36_like superfamily.
pfam13385	Laminin_G_3	3.80e-10	97	282	5	149	Concanavalin A-like lectin/glucanases superfamily. This domain belongs to the Concanavalin A-like lectin/glucanases superfamily.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BBF45384.1	0.0	25	1325	21	1319
BAK20929.1	0.0	437	1326	60	954
AIM25582.1	0.0	437	1326	60	954
BBC61544.1	0.0	437	1326	60	954
BAL62658.1	9.71e-241	683	1326	1	650

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5U22_A	5.12e-33	453	843	38	421	Structureof N2152 from Neocallimastix frontalis [Neocallimastix frontalis]
2WZ8_A	4.01e-16	1194	1325	32	147	ChainA, Cellulosome Protein Dockerin Type I [Acetivibrio thermocellus]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000217	0.999091	0.000183	0.000168	0.000160	0.000144

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003215_00985.