CAZyme Information: MGYG000003245_00342

Basic Information

• Species: Mediterraneibacter sp900761655
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Mediterraneibacter; Mediterraneibacter sp900761655
• CAZyme ID: MGYG000003245_00342
• CAZy Family: GH95
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1700		185884.29	4.0843

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003245	2245318	MAG	United States	North America

• Gene Location: Start: 2720; End: 7822 Strand: +

Full Sequence      

MRRKGKKSIISIAAAVALAVTVVPDTTVMAAPEPGSEAKPLKLQYFSPADKGGSERENWK
RWALPLGNGHMGAMVFGRTDTERIQLNEKTLWSGGTGGTDDAEGGEYDARDPQSDAYGNV
DSYGSGAMESYIDFLFDEYYSGSTAGNGPAQSGAMKILPNNRSALGDYQNFAEMYIDFDH
PVEKTSDYVRELDLRTALSTVSYEYEGVEYTREMFANYPDNVLVYRVTADEGESVDLTLR
PEIADLGTTSGGHSKKITKEGTVIADEDSKTITMEGHIENNGMKFAGMFRVENEGGEVTA
DNSDETGGMLSVEGADSVVIYVALATNYENDWPDYRQEDADYAIKSVTERIENASGKKYD
KLYEDHLADYQELFSRVELDLGGTYNADEETDKLLSAWNSNSSSGTQNHYLEELYFQYGR
YMLIASSRSDTLPSNLQGIWNDRAFADWQSDYHTNINLQMNYWAAYSTNLAEIGESLNGY
VESLTEPGQLTANKLFGTTGDAWMVNCSANALGFTGNINSNASLAVTANAFILQNVFDYY
EFTQDKDTLANEIYPVMTGACDFFLQLLLNGRTEADQDKLFMAPSYSSEQGPWTIGATYD
QQLIYLLFAETLEASKELGISNAFTQELEDAMERLYPINIGESGQIKEWQQEGAYNRYEG
TGTKIGSDDHRHNSMLMVLHPGNLITTETPELMEAAKTTLKLRGDGATGWSMGQKFNMWA
RLQDGNHAYTLFKNLMKNGTFTNLFDYHTPDIFQIDGNFGATAGIAELLVQSHAGYVSIL
PALPDDLAEGSVDGLTAEGNFVVDLDWADGKMTELEITSRTGNELSLKAGVVEKIIDTTS
GMEVTEVMQDSDGAVTFATEAGHTYQIIPGEEQGLLRAEKVVEEVRNMDIYAYEYTTATV
NEFTEAFLACQRMTEQENYMVSGIDKTVDRLLNAVEMLEMREGGEYASAAVIRFLENAAK
VNYKGTDTEDQWRQNIEYDRSDLTEDLEGEQADPAELVNTAKELLKTANEIAGYSDYRIT
LFDLICKAKQVEQGTRTDEEWAAYHAAVEDVVRIFANPRAEENDLKAAYDELKEVIETME
ETFTIHASAEGRGKIDPSGEVTVLGGASQKFTVVPDENAQILDVLINDKSVGAVPEYIFE
NVSGDGQIQALFNDRTKSEAEALLDTALSRAANITDKSLYKSEGWSVYQNALASAGEADR
TDEADMKAKATALLEAMDGLYAWGSATRTEGESMVVAAGYGDQALGGEKLLAGYPDGGPV
AASGTGANGRWTKQSGGDYSTTLSGGAQMLSKTSGAWVKFNFTGERVVFISEEAQQGAQL
DIYIDGVPVDTIKSWTGTSSINNRQTPVFDSEDYDLEALGIDMSKESHELKIVGTTGNQG
SYTFPIFRVDAFDEYLNAEKTASTEELAKLIAEVSALVKTSYTAESWNKLQTALEAAESV
MTDPSSSDTQVEEAYNGLKSAKDELKSANAKITEVTGLMPVSVPYGTSSDDLEKLLPESV
VVTEENGQKIRINVEWNLDEFDGSTAGEVTLEGKLAEIEKYGLKNPDNLMAVLNVEVRKA
EAKIILSNLEQTEGEVTAVIADTEPEGLAVRILYDGLEILPTEAGEYLVTAEITDDNYTG
KATATLVIKEKTVEKPEQPGGSNDDDIQGGNNSGSQQDEGKDTAVKTGDSQNVWLPIAGL
GIALIGAAAAGSMRYIRRKK

Enzyme Prediction     

EC	3.2.1.63

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH95	37	830	1.1e-234	0.9847645429362881

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam14498	Glyco_hyd_65N_2	8.16e-60	43	330	1	233	Glycosyl hydrolase family 65, N-terminal domain. This domain represents a domain found to the N-terminus of the glycosyl hydrolase 65 family catalytic domain.
pfam18887	MBG_3	5.19e-15	1562	1632	1	72	MBG domain. This entry corresponds to an MBG (mirror beta grasp) domain. It is found in a variety of bacterial cell surface proteins.
pfam07554	FIVAR	0.004	1405	1465	1	68	FIVAR domain. This domain is found in a wide variety of contexts, but mostly occurring in cell wall associated proteins. A lack of conserved catalytic residues suggests that it is a binding domain. From context, possible substrates are hyaluronate or fibronectin (personal obs: C Yeats). This is further evidenced by. Possibly the exact substrate is N-acetyl glucosamine. Finding it in the same protein as pfam05089 further supports this proposal. It is found in the C-terminal part of Bacillus sp. Gellan lyase, which is removed during maturation. Some of the proteins it is found in are involved in methicillin resistance. The name FIVAR derives from Found In Various Architectures.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ASZ20325.1	8.34e-197	34	867	40	803
QPA42690.1	9.10e-197	34	867	40	803
QPA36938.1	9.10e-197	34	867	40	803
QHH85405.1	1.16e-196	34	867	40	803
QRH05335.1	1.16e-196	34	867	40	803

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2EAB_A	6.38e-143	35	867	12	894	Crystalstructure of 1,2-a-L-fucosidase from Bifidobacterium bifidum (apo form) [Bifidobacterium bifidum],2EAB_B Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum (apo form) [Bifidobacterium bifidum],2EAC_A Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum in complex with deoxyfuconojirimycin [Bifidobacterium bifidum],2EAC_B Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum in complex with deoxyfuconojirimycin [Bifidobacterium bifidum]
2EAD_A	4.45e-142	35	867	12	894	ChainA, Alpha-fucosidase [Bifidobacterium bifidum],2EAD_B Chain B, Alpha-fucosidase [Bifidobacterium bifidum]
2EAE_A	8.27e-142	35	867	11	893	ChainA, Alpha-fucosidase [Bifidobacterium bifidum]
2RDY_A	3.10e-120	41	865	3	787	ChainA, BH0842 protein [Halalkalibacterium halodurans C-125],2RDY_B Chain B, BH0842 protein [Halalkalibacterium halodurans C-125]
4UFC_A	1.39e-116	41	831	22	745	Crystalstructure of the GH95 enzyme BACOVA_03438 [Bacteroides ovatus],4UFC_B Crystal structure of the GH95 enzyme BACOVA_03438 [Bacteroides ovatus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8L7W8	4.94e-94	38	819	49	804	Alpha-L-fucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=FUC95A PE=1 SV=1
A2R797	4.67e-76	63	809	38	758	Probable alpha-fucosidase A OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=afcA PE=3 SV=1
Q5AU81	1.78e-73	36	807	15	775	Alpha-fucosidase A OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=afcA PE=1 SV=1
Q2USL3	6.55e-63	63	823	32	711	Probable alpha-fucosidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=afcA PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000292	0.998988	0.000165	0.000198	0.000171	0.000153

TMHMM  Annotations     

start	end
9	31
1674	1696