CAZyme Information: MGYG000003259_00184

Basic Information

• Species: HGM14224 sp900761905
• Lineage: Bacteria; Firmicutes_G; SHA-98; DTUO25; HGM14224; HGM14224; HGM14224 sp900761905
• CAZyme ID: MGYG000003259_00184
• CAZy Family: GT51
• CAZyme Description: Penicillin-binding protein 2D

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
732		79905.63	4.6172

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003259	2214959	MAG	United States	North America

• Gene Location: Start: 1746; End: 3944 Strand: +

Full Sequence      

MKSKAKKIFLGFLIACLVIFLIGVGVAAAVLTEYLRNAPSVEDLEIVPNLPTIVYDVNGE
VIARFMTENRIMMDIEDMPQDLKNAVVAIEDHTFYEHNGISIRRIVGALLADIKNRNLDQ
GGSTITIQLARNVFLSHEKKLSRKIWEVLYAFQLERRYTKDEILEYYMNYVYFGHGAYGV
EAASLLYFGKSVNDLSLAEMALLAGLPQNGNKLSPYVNMEGAVRRRNTVLNRMAELGYIT
TEQAEEAKSEEVTLVGLKSVDPTARYFRYMVRDYLLDEYGSNMTYNGGLSVYTTLDLDMQ
RAAEAAVKQHLPNGRVQENSDGTTLTYPQCALVSLDPSTGEIRAIVGGRGEDEYNRATKA
FRTPGSSFKPFLYTAAIDNGFAPATVMIDEPISFEDSNHNVWAPKNFNNRFLGEMTLRYA
LEQSTNTIAVQLADQLTMKTVISYAKKMGITTLVESGAVNDLGLATALGGLTKGVSPIEM
AGAYAVLANQGIRITPYFITEVVDRNGLVIESHKPERELVLDEKTAYVVTDMMRGVITSG
TGRSANIGRPAAGKTGTSDDNTDVWFVGFTPDLVTSVWVGEDTRKAMSYSGIGVIGSIYP
VRIWSTYMKAALADTPANDFPVPEGIVTLTVCKDSGLLASDDCPEERVITDIFIEGKAPT
EKCNVHQPPPSPIQNFFNNLFGGNGNGDGSSATTADPSADPSNVQPVNVDTGIEVPEVQE
VTTPVAPEGTAG

Enzyme Prediction     

No EC number prediction in MGYG000003259_00184.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	58	233	4.3e-67	0.9887005649717514

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0744	MrcB	0.0	1	629	5	618	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
COG5009	MrcA	0.0	6	674	2	779	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	0.0	69	614	1	531	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
TIGR02071	PBP_1b	1.54e-119	35	659	117	726	penicillin-binding protein 1B. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of a particular bifunctional transglycosylase/transpeptidase in E. coli and other Proteobacteria, designated penicillin-binding protein 1B. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
PRK11636	mrcA	1.25e-114	7	662	5	831	penicillin-binding protein 1a; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADL11952.1	4.07e-196	48	668	45	693
AZR73658.1	3.13e-195	1	668	1	684
BBB92852.1	1.39e-191	13	661	27	660
AJQ27844.1	1.36e-190	17	627	28	629
AIF51433.1	3.07e-189	25	675	37	686

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4OON_A	1.32e-113	50	644	19	755	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
3UDF_A	1.14e-90	51	617	20	721	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
3ZG8_B	1.71e-78	153	589	2	440	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]
3ZG7_B	3.63e-73	153	589	2	440	CrystalStructure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the apo form [Listeria monocytogenes]
5FGZ_A	5.71e-73	48	640	142	720	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O66874	5.22e-126	6	664	2	717	Penicillin-binding protein 1A OS=Aquifex aeolicus (strain VF5) OX=224324 GN=mrcA PE=1 SV=1
Q07806	1.73e-114	25	644	17	782	Penicillin-binding protein 1A OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=mrcA PE=1 SV=2
O87579	2.26e-111	17	627	16	721	Penicillin-binding protein 1A OS=Neisseria lactamica OX=486 GN=mrcA PE=3 SV=1
Q5FAC7	8.71e-111	23	628	20	722	Penicillin-binding protein 1A OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) OX=242231 GN=mrcA PE=3 SV=3
O05131	8.71e-111	23	628	20	722	Penicillin-binding protein 1A OS=Neisseria gonorrhoeae OX=485 GN=mrcA PE=1 SV=3

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.617432	0.318228	0.049694	0.002142	0.001135	0.011372

TMHMM  Annotations     

start	end
9	31