CAZyme Information: MGYG000003269_00433

Basic Information

• Species: CAG-110 sp900762245
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Oscillospiraceae; CAG-110; CAG-110 sp900762245
• CAZyme ID: MGYG000003269_00433
• CAZy Family: GH78
• CAZyme Description: 6-phospho-beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1148		130522.24	6.1926

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003269	2173466	MAG	El Salvador	South America

• Gene Location: Start: 12943; End: 16389 Strand: -

Full Sequence      

MELLRNSLITSTQPYAEGAVYVFEQPFEITEEGKLTVTALGVYEAEVDGIKLGTQLFAPG
FTYYPTGLYYQTYDLSYLEPGKHTLRVYLAQGWYCGRFTFDNKVQIYGEKAAVSWALEAG
ERVYTSADDSVVELESPYEYAGFYDGEIYHSKELTVIGKPVKSATSLPEHISETTCAVRI
REEMPVKSITVNGDTTIIDFGQNFAGIVCIDPARMKGESLNLRHGEILNSDGTLYTANLR
KAKAETVYHKGNSTESYRPRFTYMGFRYVELTGVPYEEGLLKAYAIYSDMERTGYFQCEN
PLVQRLYENQIWGQKSNYVEVPTDCPQRDERMGYTGDGQVFAHTGAYNYDTQIFWDKFFK
DIRHSQSANKEGYVAPTIPASPGEQGIGFMSMLGWGNCICIVPELLYHHFGTDRFIRENY
DAMKTFVDCEIRHMGQGILGKKDLWIQPSLGDWLAMGRDVGYMAMHNGPVSNAFIVNDLR
IMAWAAKLLEKTEEAEKYAAQLEKSRDAYIKAFVRSNGKMKDDYQGAYVMALQMVIPKGE
LWNKVFSHLVSDIKKNGMQTGFFATEHILPLLTENGEEKLVFDLLLSEKCPGWLYQVKAG
ATTTWERWDALRPDGTVNETKMSNDNMVSFNHYSFGSVGKFYYQYILGIQPLEPGFAKVK
IAPHFDERIGPFSGSYKDIKVKWDGEDLHVTTPVEAEIHFPGAKVQTVKPGSYRIYKKVK
TMKFPKDFLLGAATAAHQVEGNNIHSDYWTMEQMKSTSFVEPSGDAVDHYNRYEEDIKLL
ANAGLNAYRFSIEWARIEPEEGKFDASELEHYRSVIRCCKENGVEPIVTLHHFTSPKWLI
SKGGWESEATVEYFKRYCVYIAENLGSELNYVVTINEANMGLQLAAIAKRYMKQLLLPKK
KKETDGQAQVGINLKQMLANQRIATAENVEVFGCEKLHTFVSQRSVEGDLLIMSAHCTAR
DSMKAICPHLKVGLSLSLHDLQPEKGGEKHAAKQWHLEFGHYLPYIKDDDFLGIQNYSRE
RFGRFGHIDPPADAELTQMNYEFYPEAIANVLRAVHKEFRGDLIVTENGTAMTDDARRVE
FIRRATDGVAKCIADGVPVKGYCYWSLLDNFEWQKGFSMTFGLIAVDRKTQTRFPKESLH
YLGKLWQK

Enzyme Prediction     

EC	3.2.1.21

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH78	192	697	3.3e-134	0.9801587301587301
GH1	722	1146	8.4e-114	0.9813519813519813

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam17389	Bac_rhamnosid6H	4.17e-97	291	646	1	340	Bacterial alpha-L-rhamnosidase 6 hairpin glycosidase domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
COG2723	BglB	3.06e-83	721	1138	1	442	Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase [Carbohydrate transport and metabolism].
TIGR03356	BGL	5.27e-82	725	1141	1	425	beta-galactosidase.
pfam00232	Glyco_hydro_1	2.83e-69	724	1138	5	440	Glycosyl hydrolase family 1.
PRK13511	PRK13511	4.66e-54	720	1145	1	462	6-phospho-beta-galactosidase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AEN97241.1	1.16e-302	1	713	5	728
QOG29430.1	4.04e-278	1	713	1	727
QZT16455.1	8.87e-277	1	714	1	730
AIQ16637.1	5.64e-276	1	713	5	749
AEB06434.1	5.00e-246	4	713	6	734

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4R27_A	6.67e-110	721	1143	4	406	Crystalstructure of beta-glycosidase BGL167 [Microbacterium sp. Gsoil167],4R27_B Crystal structure of beta-glycosidase BGL167 [Microbacterium sp. Gsoil167]
6GSZ_A	1.44e-101	8	712	116	858	Crystalstructure of native alfa-L-rhamnosidase from Aspergillus terreus [Aspergillus terreus]
3W5M_A	7.38e-100	23	663	309	947	CrystalStructure of Streptomyces avermitilis alpha-L-rhamnosidase [Streptomyces avermitilis MA-4680 = NBRC 14893],3W5N_A Crystal Structure of Streptomyces avermitilis alpha-L-rhamnosidase complexed with L-rhamnose [Streptomyces avermitilis MA-4680 = NBRC 14893]
6IER_A	2.23e-87	728	1145	36	427	Apostructure of a beta-glucosidase 1317 [uncultured bacterium]
6I60_A	6.38e-75	22	718	178	917	Structureof alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12],6I60_B Structure of alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q82PP4	3.24e-99	23	663	309	947	Alpha-L-rhamnosidase OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=SAVERM_828 PE=1 SV=1
T2KNB2	4.52e-87	22	709	180	887	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22090 PE=1 SV=2
P9WF03	4.56e-80	32	700	185	876	Alpha-L-rhamnosidase OS=Alteromonas sp. (strain LOR) OX=1537994 GN=LOR_34 PE=1 SV=1
T2KPL4	6.26e-51	4	695	154	895	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22170 PE=2 SV=1
P10482	1.04e-49	722	1141	3	446	Beta-glucosidase A OS=Caldicellulosiruptor saccharolyticus OX=44001 GN=bglA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000061	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003269_00433.