CAZyme Information: MGYG000003277_01657

Basic Information

• Species: Duncaniella sp900762315
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Muribaculaceae; Duncaniella; Duncaniella sp900762315
• CAZyme ID: MGYG000003277_01657
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
877		100237.75	5.6732

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003277	3767547	MAG	United States	North America

• Gene Location: Start: 12135; End: 14768 Strand: -

Full Sequence      

MKKSTILAALALAAVALSGNAEVATRQLHDGWRFRQQRSDNWFPATVPGTVHTDLMANDL
IEDPFYRLNERTVQWVDKEDWWYETTFTATPDEVDAENQELVFLGLDTYAKIYLNHQLID
YTNNMHRTWRYNVKGILKEGENLLEVQFDSPIKVDLPKYDQFDYTFNTGPDQSQNGGIFN
KTLSIFARKAGYHYGWDWGPRLVTSGIWRPVQLETWTGQRISDVQYIQEDVNAKRAKLST
VVEIRSDVDAPDALVTITADGKEVATKKTELKKGVNTVTLDYTVNKPRLWWTNGLGEPYL
YNFTTSLSVDGNKVEAQDQEIGIRSLKLIHHPDAQGMCLYFELNGKPVFAKGADMVPLDN
FLPRITREKYEKHVLDAKNVNMNMIRVWGGGVYEDDYFYQLCDRNGILVWQDFMFACSTY
PADSAFIANIRQEAIDNVKRLRNHCSIALWCGNNECQDVYYGWGGRQKYYIEKGVEELTT
KQFKDMYFRCLPEVVKEYGGGIAYRPSSPFAFEDTASDGVNGDSHYWGVWHGRDSIGHYN
VEKSRFFSEYGFQSFPEFESVKIYAPEERDWAITSEAMMCHQRAGSYANGLIRDYMDQEY
RRPEDFPSFLYVGSVLQGDAIKTAFEAHRRDMPHCMGTLVWQHNDCWPVASWAARDYYGR
WKAQQYYSKYALDDILVSPVVMNDTLTVNIVTDRRTPAKGKFTLTAMTLDGKPVWEKSFN
YNAKPLTSTQVFRDKVGNLLNGLDRRDVIFVTTFATGDRVYNNVGYATKQKNMNYGRPEL
KIDIAKADGGYDVTLGSDVFARAVFMSLDGIDNFFSDNYFDILPGAKRTIHVTTPLSRAD
FEKQLKVISMGDVHAQVAATEAAKLDNSGEFKPLGGN

Enzyme Prediction     

EC	3.2.1.25

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	23	731	4.4e-108	0.6901595744680851

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.60e-101	28	834	15	786	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam17753	Ig_mannosidase	9.09e-22	777	852	1	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
PRK10340	ebgA	2.52e-17	83	455	113	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam00703	Glyco_hydro_2	1.72e-14	220	324	2	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
PRK10150	PRK10150	5.41e-14	22	455	6	417	beta-D-glucuronidase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ALJ60279.1	0.0	1	852	8	863
QUT88714.1	0.0	1	852	8	863
QDO68488.1	0.0	26	852	31	863
QUT74423.1	0.0	26	852	31	862
QUT99864.1	0.0	22	852	4	838

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VJX_A	7.46e-211	19	835	2	819	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	7.94e-211	19	835	4	821	Structureof a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	8.20e-211	19	835	4	821	ChainA, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	2.17e-210	19	835	2	819	Structureof the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]
2VQU_A	9.39e-209	19	835	2	819	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQU_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A2QYN2	9.08e-126	21	833	2	829	Beta-mannosidase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=mndB PE=3 SV=1
Q0CCA0	2.43e-125	26	833	7	827	Beta-mannosidase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndB PE=3 SV=2
B8NW36	6.84e-125	28	833	9	828	Beta-mannosidase B OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=mndB PE=3 SV=1
Q2TXB7	1.88e-124	28	833	9	828	Beta-mannosidase B OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=mndB PE=3 SV=3
Q4WAH4	7.39e-124	28	677	9	658	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000367	0.998693	0.000254	0.000235	0.000223	0.000203

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003277_01657.