CAZyme Information: MGYG000003301_00569

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; F0040
• CAZyme ID: MGYG000003301_00569
• CAZy Family: GH2
• CAZyme Description: Evolved beta-galactosidase subunit alpha

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1351		152167.92	7.6561

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003301	1673150	MAG	Mongolia	Asia

• Gene Location: Start: 543; End: 4598 Strand: +

Full Sequence      

MNRKITHALLLAAVLSCSAAQADNRPMPGFRYDRNVTAPTGKEWEDPTMPALNKERPHAW
FFSFADEKQALNVLPESSPYWKSLDGEWSFNWVGNPGERPADFYKTNYDISGWDKVQVPM
NWNVVGIQKDGTQKYGTPIYTNQRVIFQHQVAVGDWKGGVMREPRKDWPTFKHRNEVGSY
RRTFTVPAEWDGREVYLNFDGVDSFFYLYINGRYVGFSKNSRNLASFDISPYLVKGENTV
AVEVYRNSDGSFLESQDMFRLPGIFRTVALTAKPKVQVRDVVAIPDFDANLVNASLNITA
SVANLTKKDVKGLRVDYKLYEKALYGEATTPVKGAEASVPVAVNKNGETDAKVVLQAGAL
VKKWSAEAPWRYVLVGELKDAKGRVLETFSTVVGFRKIEIKETAAADDEFGKAGRYYYLN
GKPIKMKGVNRHETNPERGHAITREQMEREVMLMKRANINHVRNSHYSNDPYWYYLADKY
GIYLEDEANIESHQYYYEKESLSHVPEFRDQHVSRNLEMVHATVNHPSVCIWSLGNEAGP
GKNFVDAYNAIKEFDKSRPVQYERNNEIVDMGSNQYPSIGLTEYIAQGTDKGIKYPFHIS
EYAHSMGNAVGNLVDYWNIIESNNFIMGGAIWDWVDQAIYNYTPDGTRYWGYGGDFGDKP
NDGMFCMNGVMRPDLTPKGQYYEIKKIYQNVGVTGVDTRAGRIEIFNKNYFTNLAGYDIR
WSLYADGRPVEGMINRPLTETRLTLGPRERKTYTLDYGNYRFDPTKEYFVKIQFLLAEKQ
PWADKGYVQMEEQLPVSEASPAPSVASAQQGRVAQPTVEGNLVKVSGKGYEMTFDKLTGR
LHSLNYGGQTVIKPGFGPKLDAFRARTDNDNWMDYRWPQLGLHNLKDKATAFAQQLLKDG
TLRLSFTVESQAPYGGHDNYSNRDRNPETVYKIVDDKSKPFGPDDFKFTTNQIYTVYPDG
SVELQSYITSNNAQVQLPRIGYAMELPKELNRFAYYGRGPVNNYRDRKTGQFVEAHEGLV
GEQDIMLPKPQSMGNREDVRWCALTNAQGNGVAFVADGQMSASALPWSALELMGAAHPHQ
LPASTATHLHLDAKVAGLGGNSCGQGGPLKPDCVKGEGYRFGFLIRPVTNGQTASATKVS
LAGGRPIAMTRGEDEKLSFVSPDANRTIVYRINGGKPQEYKAPVPFRDGGKVEAWYKDDK
ATVATQTFHKIENVPLSVLFCSSQETGGEGVEHIVDNNTSTYWHTMYSVTVAQYPHWVDF
DANGTKLMKGFVYVPRQDGPNGRIKDYEIYVSQDGKNWGSPIAKGTFPNGSKQQRVDFAQ
PVRARYIRFKALSSQNGQDFASAAEFILIAD

Enzyme Prediction     

No EC number prediction in MGYG000003301_00569.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	75	1013	4.5e-203	0.9680851063829787
CBM32	1222	1347	2.7e-26	0.9112903225806451

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK09525	lacZ	0.0	42	1100	15	999	beta-galactosidase.
PRK10340	ebgA	0.0	42	1137	2	1009	cryptic beta-D-galactosidase subunit alpha; Reviewed
COG3250	LacZ	5.32e-130	74	1011	5	804	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam02836	Glyco_hydro_2_C	4.07e-79	417	691	8	300	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
smart01038	Bgal_small_N	6.49e-74	826	1125	1	272	Beta galactosidase small chain. This domain comprises the small chain of dimeric beta-galactosidases EC:3.2.1.23. This domain is also found in single chain beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QFQ12980.1	0.0	9	1351	7	1344
QRO23809.1	0.0	1	1351	1	1348
QQD14684.1	0.0	8	1351	14	1347
CDT15944.1	0.0	8	1351	14	1347
VTR31950.1	0.0	1	1351	3	1349

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6S6Z_A	2.25e-183	43	1126	3	980	Structureof beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_B Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_C Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_D Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_E Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_F Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_G Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_H Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8]
6SD0_A	2.31e-183	43	1126	4	981	Structureof beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_B Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_C Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_D Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8]
3DEC_A	3.98e-166	43	1131	6	1000	ChainA, Beta-galactosidase [Bacteroides thetaiotaomicron VPI-5482]
3BGA_A	1.47e-164	43	1131	9	1004	Crystalstructure of beta-galactosidase from Bacteroides thetaiotaomicron VPI-5482 [Bacteroides thetaiotaomicron VPI-5482],3BGA_B Crystal structure of beta-galactosidase from Bacteroides thetaiotaomicron VPI-5482 [Bacteroides thetaiotaomicron VPI-5482]
3MUY_1	2.37e-111	42	1101	14	996	Chain1, Beta-D-galactosidase [Escherichia coli K-12],3MUY_2 Chain 2, Beta-D-galactosidase [Escherichia coli K-12],3MUY_3 Chain 3, Beta-D-galactosidase [Escherichia coli K-12],3MUY_4 Chain 4, Beta-D-galactosidase [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O52847	3.04e-184	35	1105	9	1010	Beta-galactosidase OS=Priestia megaterium (strain DSM 319 / IMG 1521) OX=592022 GN=bgaM PE=3 SV=1
Q56307	1.26e-182	43	1126	4	981	Beta-galactosidase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=lacZ PE=1 SV=2
P06864	2.93e-141	44	1142	4	1011	Evolved beta-galactosidase subunit alpha OS=Escherichia coli (strain K12) OX=83333 GN=ebgA PE=1 SV=4
A5F5U6	3.51e-137	42	1101	11	992	Beta-galactosidase OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395) OX=345073 GN=lacZ PE=3 SV=2
Q9K9C6	1.97e-135	46	1114	12	998	Beta-galactosidase OS=Alkalihalobacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=lacZ PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000992	0.652964	0.345017	0.000433	0.000323	0.000266

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003301_00569.