CAZyme Information: MGYG000003306_00387

Basic Information

• Species: CAG-448 sp003150135
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; CAG-272; CAG-448; CAG-448 sp003150135
• CAZyme ID: MGYG000003306_00387
• CAZy Family: GH28
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1258	MGYG000003306_13|CGC1	136505.76	4.8326

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003306	2266147	MAG	China	Asia

• Gene Location: Start: 20677; End: 24453 Strand: -

Full Sequence      

MKKSIQRIVRTVIAATLSLAIMGAGSSAFAAESAFSQWKTDDWTEATENGTPILKSQPGK
SINVLYLKGKATGNKLSFDIRVDNSFGTVDGNVGSAFKLSDGTQYFFEYNTVGELVRIRR
LGNDGSDSHVCPGKSYKLTAEQWHTVSLEFAENHLLWQIDGETVHELSETGNDKFEGGTL
YIQSYNTDVSLRNISVESVYIPIVEKREYDFEFTSEKSVADFSARNGGVTWKNGRLIYTL
TGNDSTLTSPPISVKQGTAYSALLPLRNTVLIRMSNGTDASRIRLSYTTTDDPAYTADKS
VVLDVQPRSGDTTYYFNLSACRNLSGYLYGFRLEPIGAASGSIEIEAVTFEREAALTGNV
GKLTSCTTDGKTVTVCGTLDSAYAGREVTLYETKPENYRLDLREQDKLTSCVADGTAFTL
TVPYENAGMTRLSSLFLLGVAGEDGKPVRIGERFWIRNGDSYDGGNPYAFTLPDYTVSVL
DFGARGDGFTNDTPAIQQAIDHVSAKGGGVVVVPGDDSFYGRRYVATNIRLKDNVELHIE
TGAVIWQSPRPADYAYDVVYGHDVSIPGVNWTHAASCHNMPLIHGDQVSNVRVTGGGVIR
MQDAGGENLDSVSAGSIWTGCPYKIHLIPLGFFRCENVEISDVHLRRTNNYHINLRTCRN
IYVSGVSMWEVTCASGDGISATVGTKNITINRCFFYSNDDAVTICSTYNDPRGIAWWHAN
PDGDNCVDNLVVRHSYLVGGHGITFIPWGTDNPRLDMQEIRNVEVYDSCLGGGSSAVGAW
PDNPYFGKQPFDNTETDDFSPVKGVRIHNNRYFGACTLECIQGTDIITDCGIRSASQFRY
GDFERNVRRHKNFTSGLSNWSFQPVEGKPGGADVKNDGNNHFGVVYGDGTLCQGLWMSKG
EHTFTADVRLLAGSGALTVRDAVSGEILAEKALETGDSFAKVSLTFTLTSGRTAYLGVTH
TGSAEQAVYIDNASVTNPEFVRMKYFTETFDDAETLQLINKGFSVTDEAGNSGNQIAEVG
GGRTGIMMLTTEKEYQEFDLHYRMRFDCASAEIDANIGVSLRRTDSGNQYDIHYNPLYHF
LMIREYKGGAESILQRVDNFDLEPGKWVDVAIRVQDNTCILYMDGKPVTEFSVAGLRTGV
ISLRAYNVNCAYDDVVVAPVGTTYVTGKEELSTETDTESAQPGTETGTGDATGASAETTF
APVTATAPASDADTTAAASASGKGCNSALGGAGLLAVAATAATAVGCARVKKKNEERA

Enzyme Prediction     

No EC number prediction in MGYG000003306_00387.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH28	528	771	3.5e-21	0.5723076923076923

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG5434	Pgu1	2.34e-17	455	742	62	344	Polygalacturonase [Carbohydrate transport and metabolism].
pfam12708	Pectate_lyase_3	1.44e-05	477	506	2	31	Pectate lyase superfamily protein. This family of proteins possesses a beta helical structure like Pectate lyase. This family is most closely related to glycosyl hydrolase family 28.
PLN02218	PLN02218	6.21e-05	476	704	67	269	polygalacturonase ADPG
pfam06439	DUF1080	0.005	1016	1157	33	181	Domain of Unknown Function (DUF1080). This family has structural similarity to an endo-1,3-1,4-beta glucanase belonging to glycoside hydrolase family 16. However, the structure surrounding the active site differs from that of the endo-1,3-1,4-beta glucanase.
pfam00295	Glyco_hydro_28	0.010	583	744	48	185	Glycosyl hydrolases family 28. Glycosyl hydrolase family 28 includes polygalacturonase EC:3.2.1.15 as well as rhamnogalacturonase A(RGase A), EC:3.2.1.-. These enzymes are important in cell wall metabolism.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QGA23464.1	1.04e-190	213	917	71	750
QCD41820.1	5.98e-118	260	862	248	843
ADB48561.1	7.25e-47	467	956	20	484
ADB48560.1	4.82e-36	465	973	18	512
QGQ94767.1	1.15e-35	467	977	29	528

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3JUR_A	4.38e-12	472	706	23	267	Thecrystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima],3JUR_B The crystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima],3JUR_C The crystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima],3JUR_D The crystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima]
4MXN_A	4.59e-11	470	695	17	214	Crystalstructure of a putative glycosyl hydrolase (PARMER_00599) from Parabacteroides merdae ATCC 43184 at 1.95 A resolution [Parabacteroides merdae ATCC 43184],4MXN_B Crystal structure of a putative glycosyl hydrolase (PARMER_00599) from Parabacteroides merdae ATCC 43184 at 1.95 A resolution [Parabacteroides merdae ATCC 43184],4MXN_C Crystal structure of a putative glycosyl hydrolase (PARMER_00599) from Parabacteroides merdae ATCC 43184 at 1.95 A resolution [Parabacteroides merdae ATCC 43184],4MXN_D Crystal structure of a putative glycosyl hydrolase (PARMER_00599) from Parabacteroides merdae ATCC 43184 at 1.95 A resolution [Parabacteroides merdae ATCC 43184]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8RY29	5.70e-08	476	777	67	320	Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2
Q02096	6.16e-08	471	704	79	283	Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1
A7PZL3	2.61e-07	469	716	55	287	Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1
P05117	1.69e-06	477	767	76	350	Polygalacturonase-2 OS=Solanum lycopersicum OX=4081 GN=PG2 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000286	0.998927	0.000199	0.000210	0.000181	0.000166

TMHMM  Annotations     

start	end
12	31