CAZyme Information: MGYG000003326_00903

Basic Information

• Species: Blautia sp900541955
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Blautia; Blautia sp900541955
• CAZyme ID: MGYG000003326_00903
• CAZy Family: GH2
• CAZyme Description: Exo-beta-D-glucosaminidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
829	MGYG000003326_19|CGC1	96251.45	4.7392

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003326	2715873	MAG	United States	North America

• Gene Location: Start: 12768; End: 15257 Strand: -

Full Sequence      

MIKQSLNQNWFLHKNNCATPFPATIPTSVYTVLADNGQIPDPYWKGNEDVVRDVIDEDYT
YTCTFSPLPELLEEDACILRFDGIDTISDVYFNDILLGQTANMHRTWEFDVTSLLLREKN
QLKVVLHSPLKAADEAFAQCATRGSEDAWEGFSHIRKAHYMYGWDWGAHLPDAGIFRDVT
LVGVKKARIDSVYVTQKHEENKVTLHFAPSFLLPHQPEKELSLECLNSTANEDFTYSVTV
TDPNGESQTLSGNPTQMEILSPMLWFPNGLGAQPLYTVKLELYARTDLVDTWERRIGLRT
LTMCVEKDEWGETFAHVVNGVKVFAMGADYIPEEHLLGKINSAKRRQLLMDAKLANFNSI
RVWGGGYYPDDEFYDLCDELGLIVWEDFMFACSVYELTPEFEANITQEFIDNIKRLRHHA
SLGLWCGNNEMESFVKDGEWVSKPSEVRDYLFMYERIIPQVLKKYDPETFYWPSSPSSGG
SFEEPQDPNRGDVHFWQVWHGNKPFSEYRKYFFRYLSEFGFQAFPSLKTIEEGISDDPQD
WNIFSYVMEKHQRNNAANGKIMGYLQQTYKYPYDFSSLIYASQLLQADGIRYGVEHFRRN
RGRCMGAVYWQLNDCWPVTSWSSIDYYGRWKALHYYAKRFFAPVMISCEEQNWMTASADM
NRQHFTFEKSIRLNVTNETFEEKKLTVRWALRNADASVVREGKTDVCVSPFSALWMDKVL
LPEADVFHQYVSYEAVENDTVISFGTVIFSYPKYFCYENPNLQMRVEGDEIIVQADTYAK
SVEILNENEDLVLEDNYFDMNGGSRRVKILRGEPEKLRVRSVYDIGRME

Enzyme Prediction     

No EC number prediction in MGYG000003326_00903.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	3	710	1.5e-102	0.6914893617021277

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	5.05e-93	3	727	12	704	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	1.09e-13	61	430	113	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK10150	PRK10150	1.74e-09	51	467	60	441	beta-D-glucuronidase; Provisional
pfam17753	Ig_mannosidase	1.95e-08	759	824	1	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
pfam00703	Glyco_hydro_2	1.18e-07	187	299	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AWY99193.1	0.0	1	828	1	828
QQQ91743.1	0.0	1	827	1	827
ASU31201.1	0.0	1	827	1	827
ANU78391.1	0.0	1	827	1	827
QJU15800.1	0.0	1	827	1	827

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VJX_A	2.33e-147	6	652	11	662	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	2.46e-147	6	652	13	664	Structureof a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	2.53e-147	6	652	13	664	ChainA, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	6.67e-147	6	652	11	662	Structureof the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]
2VQU_A	2.75e-145	6	652	11	662	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQU_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q0CCA0	2.51e-103	2	708	5	728	Beta-mannosidase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndB PE=3 SV=2
A1CGA8	2.62e-103	20	812	28	830	Beta-mannosidase B OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=mndB PE=3 SV=1
Q5B7W2	3.71e-100	24	658	31	672	Beta-mannosidase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mndB PE=1 SV=2
Q4WAH4	5.46e-99	6	708	9	730	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1
B0YBU9	2.05e-98	6	708	9	730	Beta-mannosidase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=mndB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000053	0.000006	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003326_00903.