dbCAN-seq: a database of CAZyme sequence and annotation

Basic Information help

Species

Ruminococcus_C sp900765125

Lineage

Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Ruminococcus_C; Ruminococcus_C sp900765125

CAZyme ID

MGYG000003337_00261

CAZy Family

GH31

CAZyme Description

Oligosaccharide 4-alpha-D-glucosyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
381	MGYG000003337_33\|CGC1	44463.87	4.84

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003337	2462692	MAG	China	Asia

Gene Location

Start: 1806; End: 2951 Strand: -

Enzyme Prediction help

EC	3.2.1.20

CAZyme Signature Domains help

Family	Start	End	Evalue	family coverage
GH31	2	333	3.6e-82	0.65807962529274

CDD Domains download full data without filtering help

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06604	GH31_glucosidase_II_MalA	3.66e-98	2	239	130	335	Alpha-glucosidase II-like. Alpha-glucosidase II (alpha-D-glucoside glucohydrolase) is a glycosyl hydrolase family 31 (GH31) enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This subgroup also includes the MalA alpha-glucosidase from Sulfolobus solfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source.
pfam01055	Glyco_hydro_31	1.33e-91	2	333	149	442	Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases.
COG1501	YicI	6.10e-69	3	371	386	711	Alpha-glucosidase, glycosyl hydrolase family GH31 [Carbohydrate transport and metabolism].
cd06603	GH31_GANC_GANAB_alpha	2.58e-60	1	369	129	467	neutral alpha-glucosidase C, neutral alpha-glucosidase AB. This subgroup includes the closely related glycosyl hydrolase family 31 (GH31) isozymes, neutral alpha-glucosidase C (GANC) and the alpha subunit of heterodimeric neutral alpha-glucosidase AB (GANAB). Initially distinguished on the basis of differences in electrophoretic mobility in starch gel, GANC and GANAB have been shown to have other differences, including those of substrate specificity. GANC and GANAB are key enzymes in glycogen metabolism that hydrolyze terminal, non-reducing 1,4-linked alpha-D-glucose residues from glycogen in the endoplasmic reticulum. The GANC/GANAB family includes the alpha-glucosidase II (ModA) from Dictyostelium discoideum as well as the alpha-glucosidase II (GLS2, or ROT2 - Reversal of TOR2 lethality protein 2) from Saccharomyces cerevisiae.
cd06602	GH31_MGAM_SI_GAA	1.77e-44	24	239	155	366	maltase-glucoamylase, sucrase-isomaltase, lysosomal acid alpha-glucosidase. This subgroup includes the following three closely related glycosyl hydrolase family 31 (GH31) enzymes: maltase-glucoamylase (MGAM), sucrase-isomaltase (SI), and lysosomal acid alpha-glucosidase (GAA), also known as acid-maltase. MGAM is one of the two enzymes responsible for catalyzing the last glucose-releasing step in starch digestion. SI is implicated in the digestion of dietary starch and major disaccharides such as sucrose and isomaltose, while GAA degrades glycogen in the lysosome, cleaving both alpha-1,4 and alpha-1,6 glucosidic linkages. MGAM and SI are anchored to small-intestinal brush-border epithelial cells. The absence of SI from the brush border membrane or its malfunction is associated with malabsorption disorders such as congenital sucrase-isomaltase deficiency (CSID). The domain architectures of MGAM and SI include two tandem GH31 catalytic domains, an N-terminal domain found near the membrane-bound end, and a C-terminal luminal domain. Both of the tandem GH31 domains of MGAM and SI are included in this family. The domain architecture of GAA includes an N-terminal TFF (trefoil factor family) domain in addition to the GH31 catalytic domain. Deficient GAA expression causes Pompe disease, an autosomal recessive genetic disorder also known as glycogen storage disease type II (GSDII).

CAZyme Hits help

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADL35590.1	3.62e-164	2	370	281	652
QRT48464.1	7.23e-164	1	375	280	655
CBL25050.1	1.17e-162	1	375	280	655
CBL17734.1	4.14e-162	2	371	279	649
QEH67919.1	1.01e-159	2	371	283	656

PDB Hits download full data without filtering help

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3NUK_A	1.22e-161	1	375	283	658	ChainA, ALPHA-GLUCOSIDASE [Blautia obeum ATCC 29174],3NUK_B Chain B, ALPHA-GLUCOSIDASE [Blautia obeum ATCC 29174],3PHA_A The crystal structure of the W169Y mutant of alpha-glucosidase (gh31 family) from Ruminococcus obeum atcc 29174 in complex with acarbose [Blautia obeum ATCC 29174],3PHA_B The crystal structure of the W169Y mutant of alpha-glucosidase (gh31 family) from Ruminococcus obeum atcc 29174 in complex with acarbose [Blautia obeum ATCC 29174],3PHA_C The crystal structure of the W169Y mutant of alpha-glucosidase (gh31 family) from Ruminococcus obeum atcc 29174 in complex with acarbose [Blautia obeum ATCC 29174],3PHA_D The crystal structure of the W169Y mutant of alpha-glucosidase (gh31 family) from Ruminococcus obeum atcc 29174 in complex with acarbose [Blautia obeum ATCC 29174],6C9Z_A Chain A, Glycosyl hydrolase, family 31 [Blautia obeum ATCC 29174],6C9Z_B Chain B, Glycosyl hydrolase, family 31 [Blautia obeum ATCC 29174],6CA3_A Chain A, Glycosyl hydrolase, family 31 [Blautia obeum ATCC 29174],6CA3_B Chain B, Glycosyl hydrolase, family 31 [Blautia obeum ATCC 29174]
6C9X_A	1.22e-161	1	375	283	658	ChainA, Glycosyl hydrolase, family 31 [Blautia obeum ATCC 29174],6C9X_B Chain B, Glycosyl hydrolase, family 31 [Blautia obeum ATCC 29174]
6CA1_A	1.22e-161	1	375	283	658	ChainA, Glycosyl hydrolase, family 31 [Blautia obeum ATCC 29174],6CA1_B Chain B, Glycosyl hydrolase, family 31 [Blautia obeum ATCC 29174]
3M46_A	1.22e-161	1	375	283	658	ChainA, Uncharacterized protein [Blautia obeum ATCC 29174],3M46_B Chain B, Uncharacterized protein [Blautia obeum ATCC 29174]
3M6D_A	1.96e-160	1	375	283	658	ChainA, Uncharacterized protein [Blautia obeum ATCC 29174],3M6D_B Chain B, Uncharacterized protein [Blautia obeum ATCC 29174],3MKK_A Chain A, alpha-glucosidase GH31 family [Blautia obeum ATCC 29174],3MKK_B Chain B, alpha-glucosidase GH31 family [Blautia obeum ATCC 29174],3POC_A The crystal structure of the D307A mutant of alpha-Glucosidase (FAMILY 31) from Ruminococcus obeum ATCC 29174 in complex with acarbose [Blautia obeum ATCC 29174],3POC_B The crystal structure of the D307A mutant of alpha-Glucosidase (FAMILY 31) from Ruminococcus obeum ATCC 29174 in complex with acarbose [Blautia obeum ATCC 29174]

Swiss-Prot Hits download full data without filtering help

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9F234	1.95e-58	3	367	382	711	Alpha-glucosidase 2 OS=Bacillus thermoamyloliquefaciens OX=1425 PE=3 SV=1
O43451	7.66e-39	3	377	1388	1765	Maltase-glucoamylase OS=Homo sapiens OX=9606 GN=MGAM PE=1 SV=6
B9F676	1.33e-36	1	374	480	823	Probable glucan 1,3-alpha-glucosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0216600 PE=3 SV=1
Q9URX4	8.52e-36	82	369	614	903	Uncharacterized family 31 glucosidase C1039.11c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC1039.11c PE=3 SV=1
Q9FN05	1.11e-35	1	379	482	831	Probable glucan 1,3-alpha-glucosidase OS=Arabidopsis thaliana OX=3702 GN=PSL5 PE=1 SV=1

SignalP and Lipop Annotations help

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000064	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM Annotations help

There is no transmembrane helices in MGYG000003337_00261.

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