CAZyme Information: MGYG000003337_01967

Basic Information

• Species: Ruminococcus_C sp900765125
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Ruminococcus_C; Ruminococcus_C sp900765125
• CAZyme ID: MGYG000003337_01967
• CAZy Family: PL1
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1349		143711.25	4.8355

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003337	2462692	MAG	China	Asia

• Gene Location: Start: 6316; End: 10365 Strand: -

Full Sequence      

MGIKKYAKKALAAFLAASTLTASGSALNAMAAESESPAMNAGTMPLVTASNTVTFTESKG
YEEGAYAEWAAVDGADGYNVYCDGVQIDSMLIRQYSGYFRADAVGIKAGSHKIKVVPTKG
GSEMTSAAAETTVTSTAHTREGYAFVGGSSSGAYNDDGTLKSNAVILYITEKTKNSVSMN
VATDAKGNTTSAVGIQNILTAYQKGYEKRPLNIRCIGNITDPETLMDGDLLIKGNGASKR
LNSGVTIEGIGEDTTFNGFGLRIANASNVEVRNIGFMNCNSGEGDDCGLQQNNDHIWVHN
CDFFYGDAGSDADQVKGDGALDTKTSSYITHSYNHFWDNGKCNLQGMKSESTENYITYHH
NWYDHSDSRHPRIRTCTVHIYNNYYDGNAKYGVGVTMGASAFVENNNFRNCKYPMLISMQ
GNDIASGDGGTFSGEDGGVIKSFGNVMTGQKAYTTYVQNSTEFDAYEATSRTETVPSSVK
AKQGGTTYSNFDTASGFYSYKVDSAEDVPSIVTANAGRVNGGDFQWKFDNSVDDTSYTVN
QPLKDALVAYKDSIVAIGSGFSSGNDQPIFTTTSAKTTTSTSTTKGGAGTTTSNTPVSGD
YVHDFTANGVSSTFYTITGNLSTGKGTVNYDGKTLTQCLKMESATNIAFTAPADGNLTLV
FAEAAKGVKVDGTKYTTDANGIVTVDLKAGAHTITKGDSISLFYMVYGKTGPVITTKTTE
SSGNQTTKTTAKTTNSSGTVSDEVTGSVKVINAGGWNEMMYMTISGASDADVTSVSYSGP
TNGKLTGADFEYLVRDVDKGVRVDITGLKPGTYSLTMETTKGSITRSGIVVGAQDRSGYA
HYNYTAGVGAYNDDGTLKSNAIVLYVTEDNKDTVSVTSKDGTTVKGIGHILNSAGMDNGT
GKTANGGAPNNNNGIIAKLAADGTPLVVRIIGNVTAPDGVTVFDSVDNGGSVGDNGYMAR
MKSGKDITIEGIGTDACVNGWGFHFMAESSKPDFGKSFEVRNVSFRNVPEDCVGMEGVQE
GSTLTASVERCWIHNCEFYVPKIANPAESDKAEGDGACDFKRGQYFTNSYCYYEGYHKTN
LVGASDSNVQYHLTYHHNYWKNCESRGPLARQANIHMYNNVFDGQSSYCMNPRANAYIFS
EYNVFNDSKNPMQVKLGAVKSYMDEFTNVKGSQDGTIVTDKSTKVDTANKYANFDTDDSI
CYIPSGNYKLDTANSPDLFTNLKEIFEKDGGCMDETSISGSTGSDITVTTTTTTTTETTT
TTTKNSDETTTTTKKVEGDVRYGDLNLDAKITLVDMVYMSKYLTGAIKFNSQQAANGDCA
KDGDVTSADATALLMFLGEKIDSLPAQIG

Enzyme Prediction     

No EC number prediction in MGYG000003337_01967.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL1	958	1148	6.7e-46	0.8465346534653465
PL1	239	410	3.8e-44	0.8267326732673267
CBM77	601	703	4.6e-43	0.9902912621359223

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3866	PelB	9.59e-54	125	517	19	340	Pectate lyase [Carbohydrate transport and metabolism].
pfam18283	CBM77	5.82e-44	599	705	1	108	Carbohydrate binding module 77. This domain is the non-catalytic carbohydrate binding module 77 (CBM77) present in Ruminococcus flavefaciens. CBMs fulfil a critical targeting function in plant cell wall depolymerisation. In CBM77, a cluster of conserved basic residues (Lys1092, Lys1107 and Lys1162) confer calcium-independent recognition of homogalacturonan.
smart00656	Amb_all	1.54e-31	232	412	5	190	Amb_all domain.
COG3866	PelB	1.71e-28	961	1151	97	279	Pectate lyase [Carbohydrate transport and metabolism].
smart00656	Amb_all	5.51e-24	964	1145	15	186	Amb_all domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADU23076.1	0.0	8	1245	7	1250
ADL35105.1	6.86e-250	57	707	61	747
AWK03841.1	7.63e-220	50	826	18	819
QHJ07965.1	7.54e-202	44	705	12	666
QSW91132.1	1.94e-198	56	705	23	821

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5FU5_A	1.44e-45	596	707	3	113	Thecomplexity of the Ruminococcus flavefaciens cellulosome reflects an expansion in glycan recognition [Ruminococcus flavefaciens]
3VMV_A	1.01e-23	210	410	47	248	Crystalstructure of pectate lyase Bsp165PelA from Bacillus sp. N165 [Bacillus sp. N16-5],3VMW_A Crystal structure of pectate lyase Bsp165PelA from Bacillus sp. N165 in complex with trigalacturonate [Bacillus sp. N16-5]
1AIR_A	1.92e-15	239	426	85	275	ChainA, PECTATE LYASE C [Dickeya chrysanthemi],1O88_A Chain A, Pectate Lyase C [Dickeya chrysanthemi],1O8D_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8E_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8F_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8G_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8H_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8I_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8J_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8K_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8L_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8M_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1PLU_A Chain A, Protein (pectate Lyase C) [Dickeya chrysanthemi],2PEC_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi]
2EWE_A	4.61e-15	239	426	85	275	ChainA, Pectate lyase C [Dickeya chrysanthemi]
1VBL_A	5.98e-15	241	408	130	330	Structureof the thermostable pectate lyase PL 47 [Bacillus sp. TS-47]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
B1B6T1	5.35e-23	187	416	60	280	Pectate trisaccharide-lyase OS=Bacillus sp. OX=1409 GN=pel PE=1 SV=1
Q65DC2	5.35e-23	187	416	60	280	Pectate trisaccharide-lyase OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46) OX=279010 GN=BLi04129 PE=3 SV=1
Q8GCB2	5.35e-23	187	416	60	280	Pectate trisaccharide-lyase OS=Bacillus licheniformis OX=1402 GN=pelA PE=1 SV=1
Q5AVN4	1.57e-17	241	421	96	277	Pectate lyase A OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=plyA PE=1 SV=1
P0C1C2	2.89e-17	239	426	106	296	Pectate lyase 3 OS=Pectobacterium carotovorum OX=554 GN=pel3 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000304	0.998853	0.000232	0.000246	0.000189	0.000152

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003337_01967.