CAZyme Information: MGYG000003363_00020

Basic Information

• Species: Bacteroides sp900766195
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; Bacteroides sp900766195
• CAZyme ID: MGYG000003363_00020
• CAZy Family: GH143
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1104		127312.57	6.6557

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003363	4177575	MAG	United States	North America

• Gene Location: Start: 32037; End: 35351 Strand: -

Full Sequence      

MMNLKQFSVWMLSALVLPCMAQEKVHYTGTELSNPAYHDGQLSPAIGVHNIQIMRANREH
PSPENGNGWTYNHQPMMAYWNGKFYVHYLSDPSDEHVPPSQTFLMTSKDGYHWTNPVTLF
PIYRVPDGYTKPGRTDKAESLDAIMHQRVGFYVSKSNRLIAMGNYGVALDRKDDPNDGNG
IGRVVREIRKDGTFGPIYFIYYNHAFNEKNTDYPHFKRCKDKGFVQACQEILDNPRYRMQ
WVEEADRKDPLIPLNKEYKAYCDYTLPDGRIVSLWKHALTSISADEGNTWLEPVERAKGF
VNSNAKIWGQRLSDGTYATVYNPSEFRWPLAISLSKDGLEYTTLNLVHGEISPMRYGGNY
KSYGPQYVRGIQEGNGTPLDGDLWVAYSMNKEDIWVSHIPVPVRLEAISQANDDFLRYKT
IAELKDWNIYSLQWAPVSLDGKWLVLQDKDLHDYAKVERVIPDSKELKVSFDLMAEQNDK
GSLQMEFLDANGIACSRMELAADGFLSVKGGARYGNFMKYEANKVYRIEIVLSVSNRLIQ
IYVDGKKVGQRIFYAPVHTIRRIAFRTGDLRLFPTPETPADWYGTLPNAGEQEPLATYKI
ANFHTSSSDKDATSAFLKYKDFAHYADYFNGMEDENIVAAIPNTKASEWMEENIPLFECP
QKNLEEMFYYRWWTLRKHIEDTPVGYGMTEFLVNRSYADKYNLIACAIGHHIYESRWLRD
PVYLDQIIHTWYRGNDGGPMKKMNKFSSWNADAVFRRYLVDGNKDFLLNMAPDLEKEYAR
WESTNRLPSGLYWQGDVQDGMEESISGGRRKQYARPTINSYMYGNAKALSAIGLLAGNED
MAAKYGMKADSLKALVQDKLWNKEHGFFETLREDSLANVREAIGFIPWYFNLPDDKSLYA
GAWAQVTDDKGFSAPYGLTTAERRHPQFRTHGAGKCEWDGAIWPFATSQTLSAMINLMNN
YHQNVIVDSVFFHQMERYVESQYHRGKPYIGEYLDEVTGYWLMGDRERSRYYNHSTFNDL
VIRGIAGLHPRADNTLEVHPLVPEDQWDWFCLDNVRYHNHTISIIWDKYGNRYRHGKGLR
IFVDGKEVGKTDKLQRVVCENALN

Enzyme Prediction     

No EC number prediction in MGYG000003363_00020.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH143	20	565	4e-287	0.9892086330935251
GH142	628	1096	2.2e-222	0.9979123173277662

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam13088	BNR_2	2.10e-07	264	338	168	247	BNR repeat-like domain. This family of proteins contains BNR-like repeats suggesting these proteins may act as sialidases.
pfam01204	Trehalase	1.50e-04	819	920	308	413	Trehalase. Trehalase (EC:3.2.1.28) is known to recycle trehalose to glucose. Trehalose is a physiological hallmark of heat-shock response in yeast and protects of proteins and membranes against a variety of stresses. This family is found in conjunction with pfam07492 in fungi.
COG3408	GDB1	0.001	749	944	343	538	Glycogen debranching enzyme (alpha-1,6-glucosidase) [Carbohydrate transport and metabolism].
cd08995	GH32_EcAec43-like	0.001	75	121	3	51	Glycosyl hydrolase family 32, such as the putative glycoside hydrolase Escherichia coli Aec43 (FosGH2). This glycosyl hydrolase family 32 (GH32) subgroup includes Escherichia coli strain BEN2908 putative glycoside hydrolase Aec43 (FosGH2). GH32 enzymes cleave sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). GH32 family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QJR63716.1	0.0	2	1103	1	1103
QJR67979.1	0.0	2	1103	1	1103
QJR72315.1	0.0	2	1103	1	1103
QQY37484.1	0.0	2	1103	1	1103
ABR40380.1	0.0	2	1103	1	1103

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MQR_A	0.0	22	1103	25	1107	SialidaseBT_1020 [Bacteroides thetaiotaomicron]
5MQS_A	0.0	22	1103	25	1107	SialidaseBT_1020 [Bacteroides thetaiotaomicron]
6M5A_A	2.83e-23	644	1102	300	814	Crystalstructure of GH121 beta-L-arabinobiosidase HypBA2 from Bifidobacterium longum [Bifidobacterium longum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH9	2.54e-22	644	1102	331	845	Beta-L-arabinobiosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA2 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000201	0.999170	0.000168	0.000151	0.000141	0.000130

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003363_00020.