CAZyme Information: MGYG000003377_01471

Basic Information

• Species: Phytobacter sp002377245
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Phytobacter; Phytobacter sp002377245
• CAZyme ID: MGYG000003377_01471
• CAZy Family: GT83
• CAZyme Description: Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
553	MGYG000003377_38|CGC1	62538.91	9.3342

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003377	5122475	MAG	United States	North America

• Gene Location: Start: 20127; End: 21788 Strand: +

Full Sequence      

MTKSSRYGLAVLALFALYYLVPVEWRLLWQPDETRYAEISREMLASGNWVVPHFLDLRYF
EKPIAGYWLNSVGQWLFGSSNFAVRAGAIFSTALVACIVAGLSWRFWRDKRIALVAAAIF
LTMLEVYGIGTYAVLDPMIALWLIAAMSVFWLATQAQTRSRKALGYFALGVACAMGVMTK
GFLALAVPVLSVLPWVIVEKKWKEVLSWGWLAIFSCVLAILPWGLAIAQREPDFWHYFFW
VEHIQRFAQDDAQHKAPLWYYLPVIVLGSLPWLGLLPGTLRQAWRDHDNQRATLYLLCWI
VMPFLFFSIAKGKLLTYILPCFAPLAMLMAYYAVRAAERGCRALRINAWINIGFGAVGIV
AAFVASPWGPMKHPVWAPLEIYKAFCAAIAFFVWALAGWYSLRNTEKNWVLSALCPLGLA
LLLGFMLPNRIMEAKQPQFLVEMNRETLQRSHYVIADNVGLAAGLAWELKRSDIMLFNFG
GELKYGLDYPNAKGKLIRARDFSEWLATHRQDGITSLVVLLAKNEDVSDLALPPPTSVYV
QGRIALLEYTPLP

Enzyme Prediction     

No EC number prediction in MGYG000003377_01471.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT83	4	524	1.2e-146	0.9666666666666667

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK13279	arnT	0.0	1	549	1	550	lipid IV(A) 4-amino-4-deoxy-L-arabinosyltransferase.
COG1807	ArnT	1.38e-90	1	525	2	535	4-amino-4-deoxy-L-arabinose transferase or related glycosyltransferase of PMT family [Cell wall/membrane/envelope biogenesis].
pfam02366	PMT	4.49e-38	4	238	1	245	Dolichyl-phosphate-mannose-protein mannosyltransferase. This is a family of Dolichyl-phosphate-mannose-protein mannosyltransferase proteins EC:2.4.1.109. These proteins are responsible for O-linked glycosylation of proteins, they catalyze the reaction:- Dolichyl phosphate D-mannose + protein <=> dolichyl phosphate + O-D-mannosyl-protein. Also in this family is Drosophila rotated abdomen protein which is a putative mannosyltransferase. This family appears to be distantly related to pfam02516 (A Bateman pers. obs.). This family also contains sequences from ArnTs (4-amino-4-deoxy-L-arabinose lipid A transferase). They catalyze the addition of 4-amino-4-deoxy-l-arabinose (l-Ara4N) to the lipid A moiety of the lipopolysaccharide. This is a critical modification enabling bacteria (e.g. Escherichia coli and Salmonella typhimurium) to resist killing by antimicrobial peptides such as polymyxins. Members such as undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase are predicted to have 12 trans-membrane regions. The N-terminal portion of these proteins is hypothesized to have a conserved glycosylation activity which is shared between distantly related oligosaccharyltransferases ArnT and PglB families.
pfam13231	PMT_2	3.30e-07	62	223	2	157	Dolichyl-phosphate-mannose-protein mannosyltransferase. This family contains members that are not captured by pfam02366.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AUV02144.1	9.63e-301	1	552	1	552
AKL13213.1	1.59e-299	1	552	1	552
BBE79197.1	3.20e-299	1	553	1	553
QIH64753.1	3.20e-299	1	553	1	553
QOV69973.1	4.55e-299	1	553	1	553

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5EZM_A	4.51e-31	21	425	47	450	CrystalStructure of ArnT from Cupriavidus metallidurans in the apo state [Cupriavidus metallidurans CH34],5F15_A Crystal Structure of ArnT from Cupriavidus metallidurans bound to Undecaprenyl phosphate [Cupriavidus metallidurans CH34]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
B5XTL1	1.32e-263	3	551	2	550	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Klebsiella pneumoniae (strain 342) OX=507522 GN=arnT PE=3 SV=1
A6TF96	2.17e-262	3	551	2	550	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) OX=272620 GN=arnT PE=3 SV=1
B7LM74	2.52e-261	3	551	2	550	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CCUG 18766 / IAM 14443 / JCM 21226 / LMG 7866 / NBRC 102419 / NCTC 12128 / CDC 0568-73) OX=585054 GN=arnT PE=3 SV=2
B7N5M2	2.66e-258	3	551	2	549	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC) OX=585056 GN=arnT PE=3 SV=1
B1LLL1	1.53e-257	3	551	2	549	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Escherichia coli (strain SMS-3-5 / SECEC) OX=439855 GN=arnT PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.997812	0.001992	0.000123	0.000004	0.000003	0.000085

TMHMM  Annotations     

start	end
7	29
82	104
113	135
139	156
163	178
182	199
206	228
258	280
293	310
314	336
343	365
380	402
409	431