CAZyme Information: MGYG000003392_00391

Basic Information

• Species: Streptococcus mitis
• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Streptococcus; Streptococcus mitis
• CAZyme ID: MGYG000003392_00391
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1262		143433.67	5.7536

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003392	1810665	MAG	United States	North America

• Gene Location: Start: 1100; End: 4888 Strand: +

Full Sequence      

MRREEVLRNHWFFSQEVGKEEALAPDFHRENWQAIQVPHDWSIYNDFDQYSPAQNEGGQL
NGGQAWYRTQFYLEEDASLVSVRLLFDGVYMNAQVYINGQVLGYYPSGYTPFSYDITPYL
RNDGTANQLAVFVENQQPSSRWYSGSGIYREVKLLITDSVHLDLYGIKIESPKLKEQRQS
WVETQLESKVSNDGPQSVSVYLEQSIWYDGQQLSDWQATDSIVIESGDKHHFQQAISIFQ
PLLWDIASPHLYQLKTRLYKNGILVNEEEETFGYRYMDWQADKGFFLNGRWLKIHGVCLH
HDYGALGAVENKSAAERCLRQMKEMGVNAIRITHNPGSSILLDLSAKMGLLIQEEAFDTW
YGGKKEYDYGRFFEEEATHPEAKTGDFWSDYDLRTMIERGKNNPAIFMWSLGNEVSEANG
DAHSLKTIKRLLERVKEVDDSRFVTMGMDQFRFGDGSGGHEKIANLLDVVGLNYSEDNID
GIRKRHPKWRLYGSETSSATRTRDSYFRPAKEWVGDNRRVRNFEQSDYGNDRVPWGKTAT
ASWIADRNRLDYAGQFIWTGVDYIGEPTPWHNQNDTPVKSSYFGIVDTAGIPKNDYYLYQ
SQWLALDQQPMVHILPHWNWEIHKSYQQVVDKYGDIPVRVYSNAGFVELFLNGKSQGRKT
FQEKWTSDGRKYQEGEGSDQLYLEWRLPYQPGELHAVAYDHQGQLVAEDRVVTAGKPAKI
GLHAEKTKLEPDGQDLLYLYFDVLDKDGNWVPSASNQIHFKIEGPARIVGVDNGRQASRE
RYQAQKNGRFKRKAFHGRGVLLVQSLEQAGQVRVKASARGLETASFDLLVGEAFDCQPVK
NQRLFEIRVDKQAGLQEGDSPAIGLYPQTVDNPVNKVGNSEVIWEMSGSAQAIINQGVLH
CLSAGDLAIRAMYQGKTYQTHLQVAENTSLGQAVSARPLRLYTAKGTYPQLPSSVLVDYE
LGGAKRVKVVWEAISEEDMASFHEFTVSGQLEGLDLKASAQVCVQGISAIEPERVWTLVK
EVPHLPDRVKLVLSDGRRDTAKVTWDELKPQIYTQVGECVLTGQVAGCELPATVTIHVTD
ASVDGEVISNQWTGSNLPLVFASHSEPNHSASYLNDKVIARKKSTANTWIAKSEQASVGI
LFGDAGILKPRFVDNVTLYYVENQEYVAVDPTHIDYYVGNEPALPRTPNHLDKDSLLKQE
ENWRPVSAIRKVSSDKDEELRFEFDKVETYALRLRFENLVNPLALTELQVYAKKVKKNVD
RK

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	5	751	6.8e-102	0.7273936170212766
CBM71	1086	1258	5.9e-47	0.9073170731707317

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	6.08e-108	1	776	9	766	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam18565	Glyco_hydro2_C5	2.27e-34	720	825	1	101	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.
PRK10340	ebgA	2.75e-34	10	444	51	472	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK10150	PRK10150	7.12e-28	63	473	65	470	beta-D-glucuronidase; Provisional
pfam02837	Glyco_hydro_2_N	4.43e-24	34	157	33	168	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QBZ12533.1	0.0	1	1262	1	1262
QGS43039.1	0.0	1	1262	1	1262
BCJ11502.1	0.0	1	1262	1	1262
QBZ14322.1	0.0	1	1262	1	1262
BBP10599.1	0.0	1	1262	1	1262

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4CU6_A	0.0	10	832	21	846	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU7_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU8_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4]
4CUC_A	0.0	10	832	21	846	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA. [Streptococcus pneumoniae TIGR4]
4YPJ_A	9.63e-235	10	825	15	802	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
7CWD_A	8.07e-225	9	835	8	806	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
7NIT_A	2.64e-181	21	1219	48	1165	ChainA, Beta-galactosidase [Bifidobacterium bifidum],7NIT_B Chain B, Beta-galactosidase [Bifidobacterium bifidum],7NIT_C Chain C, Beta-galactosidase [Bifidobacterium bifidum],7NIT_D Chain D, Beta-galactosidase [Bifidobacterium bifidum],7NIT_E Chain E, Beta-galactosidase [Bifidobacterium bifidum],7NIT_F Chain F, Beta-galactosidase [Bifidobacterium bifidum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	3.98e-170	21	824	60	842	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	7.28e-97	2	774	46	768	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	3.84e-45	2	761	51	767	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
T2KN75	5.55e-42	11	828	35	793	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1
P26257	1.40e-36	1	770	1	706	Beta-galactosidase OS=Thermoanaerobacterium thermosulfurigenes OX=33950 GN=lacZ PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000055	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003392_00391.