CAZyme Information: MGYG000003396_00185

Basic Information

• Species: Clostridium sp900766315
• Lineage: Bacteria; Firmicutes_A; Clostridia; Clostridiales; Clostridiaceae; Clostridium; Clostridium sp900766315
• CAZyme ID: MGYG000003396_00185
• CAZy Family: CBM50
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2041		221568.93	4.2613

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003396	3926186	MAG	United States	North America

• Gene Location: Start: 20356; End: 26481 Strand: +

Full Sequence      

MYKITKSKFLNLFVALTMVLGLVTIPTKAVNATIATYVVISEVYGGGGNSGATLKNDFIE
LYNPTANDINLDGWKFEYASKTGTFNQSTELSGIIKAGGYYLIQQGAGAGESEELDTPDA
TGNISMSGSECKVRLVDNNGNIIDLVGIGLAANESETSPTDKMSNTTSVQRKDNNGEAIG
ETNGWDTNNNKDDFYLGTPTPRNSSYNNGGSETPDPGPEENDIISIEEARKATESVKVKG
TIIFKEVSGSYYNYALQDGSAGIALRGKDELELGDEVIVEGQPAEYKGLIQIQYTNIEKR
ASNVSLPEPKEVKITDIINNDGGEKYESQRVKFIDVTIGDINTSSNTVITDNEGNTINLY
KIPELDQISKDDKVDVIGVCSQFTSYQIRVARASEITKSQVSGETEEDRNGPTLTLVTPG
NLVTTNNKVTIKVSYKDESEIKKDSLSFLLDDINVSEKAEIGDKYITYMPEEELTVGDHK
ISLSLSDINGNNSVLEAVITVKEKVNVNGLNVYFGQLHSHTNISDGAGSIEDAYDYAKNK
SGVDFLAVTDHSNSLDNDKEASMEDGSMSPEWVNAHNVAEKYTDENFLGVYAYEMTWSNG
IGHINTFNTPGFESRNKSGLTNNDSNALQNYYNKLKEFPASLSQLNHPGKTFGDFNDFSL
YDEQIDSLVTMIEVGNGEGAIRGSGYFPSYDYYTRALDKGWHVAPTNNQDNHKGKWGNAN
TARTVILAEDLNEENLYDALRNMRTYSTEDENLSIVYTLNDEIMGSIIDKPDTVNIKVDV
KDEDNENIGKVSVLVNGGAVIDSKTLNSNEGTIEFDLPADYSYYYIRIDQEDKDIAVTAP
VWLGEVEKAGLSTTEASTTMPIKGEEFIIETSLYNNEDFPLTIDSIEYSIDGEVINSTSE
KITINSLSTGSYSFKYTPNASGSFNIDVKAKGTLNGVEKTYTDVLKITVSDPSVISKVVI
DATHFNDYVGGYYANNMGNFTAIANSEDVAVNIETNKITNELLKDVQLLIITAPAKKAGT
ANGISYEPQSFSDEFIKVVKNYADNGGNIIVTGLADYQDGTGEYASSTQINKLLEALDVT
TRINNDEVVDDDNNAGQSYRLRFKNINTNSEFTNGVVAEQEYSFYSGCSVSLNEKSVKEG
KATWLVKGFDTTYSFDSNKNTTGVSVPKGEVIALATEKLSGGGNVFVGGTVFMSDFEVQA
SLDNYGDLQYSNYNIVLNILESAKKEVAVTPIKDVRSAEMGEVFCIEGYVTAGTQEGNAF
FDTIYVQDKTAGINIFPVAGIDLNLGQKVRIIGSVSSYEGEKQINLNSIEVLEEENKIIE
PTKLTTKEATLEVNKGLLVSVTGIVKSIGADNSGYMIVNDGSGDVRVFLNGYVGSSDNSI
ETGKFNSNIKVGDTVTAIGLASTDTEGDRIRVRDSKEVVLEKSTETTITILHTNDSHGRV
KADSSVIGIDTISAIKKSISNSLLVDAGDTLHGLPFATLNKGADIVNLMKLSGYDLMTPG
NHDFNYGYERLLELAALAEEGENGFEIISANVLKDNESLLKSNSIKEIDGIKVGFFGLTT
PETAYKTNPNNVIGIEFFDPIESAKKQVASLKAEGADIVIALAHVGTDESSEVQSTMIAE
AVEGIDVIIDGHSHTVFENGYEAANNTLIVSTGEYEGNLGKLSLVVDRETKKIKVKTASL
INKEEALTYTPESTVTDAIKAIDNEQLEILSEVVGNSLVKLDGERGNVRTKETNLANLIT
DAMLYETEADIAITNGGGIRSSIDEGEITRGEIISVLPFGNFIVTKYLTGSQIKEVLEHG
VRVYPESLGGFTQVGGLKFIFDDTKPAGERVVKITVNGENIDMNKRYLVATNDFLAAGGD
EYPCFGDVATENEFRALDEALASYIESLGKVNYEVEGRITIDTLENIEAQENKAAADKVI
DLINKLPKEITLNHKNKVKEARASYEALTDIQKELIPSNILKVLEDAEAKIKVLEDNSQT
TNPGNGGNNNSSNGNQGSGNGKLPSTGGMNVFYLYLLGSILIGTGALFAVNKKKSNSVNK
Y

Enzyme Prediction     

No EC number prediction in MGYG000003396_00185.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK09419	PRK09419	1.89e-102	1421	1900	654	1144	multifunctional 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase/5'-nucleotidase.
COG0737	UshA	1.05e-91	1427	1863	26	473	2',3'-cyclic-nucleotide 2'-phosphodiesterase/5'- or 3'-nucleotidase, 5'-nucleotidase family [Nucleotide transport and metabolism, Defense mechanisms].
PRK09558	ushA	5.72e-87	1421	1899	28	548	bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor; Reviewed
cd07408	MPP_SA0022_N	3.65e-69	1428	1667	1	242	Staphylococcus aureus SA0022 and related proteins, N-terminal metallophosphatase domain. SA0022 is an uncharacterized Staphylococcus aureus UshA-like protein with two putative domains, an N-terminal metallophosphatase domain and a C-terminal nucleotidase domain. SA0022 also contains a putative C-terminal cell wall anchor domain. The N-terminal metallophosphatase domain belongs to a large superfamily of distantly related metallophosphatases (MPPs) that includes: Mre11/SbcD-like exonucleases, Dbr1-like RNA lariat debranching enzymes, YfcE-like phosphodiesterases, purple acid phosphatases (PAPs), YbbF-like UDP-2,3-diacylglucosamine hydrolases, and acid sphingomyelinases (ASMases). MPPs are functionally diverse, but all share a conserved domain with an active site consisting of two metal ions (usually manganese, iron, or zinc) coordinated with octahedral geometry by a cage of histidine, aspartate, and asparagine residues. The conserved domain is a double beta-sheet sandwich with a di-metal active site made up of residues located at the C-terminal side of the sheets. This domain is thought to allow for productive metal coordination.
cd00845	MPP_UshA_N_like	6.20e-59	1428	1681	1	253	Escherichia coli UshA-like family, N-terminal metallophosphatase domain. This family includes the bacterial enzyme UshA, and related enzymes including SoxB, CpdB, YhcR, and CD73. All members have a similar domain architecture which includes an N-terminal metallophosphatase domain and a C-terminal nucleotidase domain. The N-terminal metallophosphatase domain belongs to a large superfamily of distantly related metallophosphatases (MPPs) that includes: Mre11/SbcD-like exonucleases, Dbr1-like RNA lariat debranching enzymes, YfcE-like phosphodiesterases, purple acid phosphatases (PAPs), YbbF-like UDP-2,3-diacylglucosamine hydrolases, and acid sphingomyelinases (ASMases). MPPs are functionally diverse, but all share a conserved domain with an active site consisting of two metal ions (usually manganese, iron, or zinc) coordinated with octahedral geometry by a cage of histidine, aspartate, and asparagine residues. The conserved domain is a double beta-sheet sandwich with a di-metal active site made up of residues located at the C-terminal side of the sheets. This domain is thought to allow for productive metal coordination.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCJ95849.1	8.26e-148	1411	1901	23	508
QZY54554.1	5.21e-142	1423	1919	29	522
QQY79959.1	5.56e-142	1417	1959	28	577
QUH21387.1	8.16e-142	1426	1901	37	509
AOT71215.1	2.17e-139	1421	1901	34	511

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2Z1A_A	5.87e-66	1427	1901	29	530	Crystalstructure of 5'-nucleotidase precursor from Thermus thermophilus HB8 [Thermus thermophilus HB8]
7D0V_A	4.12e-54	1428	1899	5	522	ChainA, Snake venom 5'-nucleotidase [Naja atra],7D0V_B Chain B, Snake venom 5'-nucleotidase [Naja atra]
5H7W_A	7.37e-54	1428	1899	5	522	Crystalstructure of 5'-nucleotidase from venom of Naja atra [Naja atra],5H7W_B Crystal structure of 5'-nucleotidase from venom of Naja atra [Naja atra]
6HXW_A	3.52e-52	1428	1899	3	520	structureof human CD73 in complex with antibody IPH53 [Homo sapiens],6HXW_B structure of human CD73 in complex with antibody IPH53 [Homo sapiens]
6VC9_A	3.67e-52	1428	1899	3	520	TB19complex [Homo sapiens],6VCA_A TB38 complex [Homo sapiens],6VCA_B TB38 complex [Homo sapiens],6VCA_C TB38 complex [Homo sapiens],6VCA_D TB38 complex [Homo sapiens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O34313	2.16e-68	1393	1899	633	1174	Trifunctional nucleotide phosphoesterase protein YfkN OS=Bacillus subtilis (strain 168) OX=224308 GN=yfkN PE=1 SV=1
A9BJC1	2.83e-59	1428	1919	24	502	Mannosylglucosyl-3-phosphoglycerate phosphatase OS=Petrotoga mobilis (strain DSM 10674 / SJ95) OX=403833 GN=mggB PE=1 SV=1
A0A2I4HXH5	4.03e-53	1428	1899	5	522	Snake venom 5'-nucleotidase (Fragment) OS=Naja atra OX=8656 PE=1 SV=1
P29240	1.06e-52	1420	1899	24	546	5'-nucleotidase OS=Diplobatis ommata OX=1870830 PE=2 SV=1
B6EWW8	3.21e-52	1428	1910	44	572	Snake venom 5'-nucleotidase OS=Gloydius brevicaudus OX=259325 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.004511	0.994302	0.000469	0.000243	0.000205	0.000214

TMHMM  Annotations     

start	end
12	34
2011	2030