CAZyme Information: MGYG000003404_00305

Basic Information

• Species: Lactococcus hircilactis
• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Lactococcus; Lactococcus hircilactis
• CAZyme ID: MGYG000003404_00305
• CAZy Family: GH73
• CAZyme Description: Autolysin

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
365		37934.17	7.1239

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003404	1333590	MAG	United States	North America

• Gene Location: Start: 1809; End: 2906 Strand: +

Full Sequence      

MKNKHQFALGASVLALSVLTGAKVSALSVSSIATAATPIAHQYGLYPSVMIAQGILESSH
GQSALASQYNNIFGVKYTSGNPVYLPTKEYINGQMKTVVEPFQAYSSLYEACLAQAQMIR
GSKLYAGAWRENASSYRDATAWLQGRYATDPDYASKLNALIQELGLSAYDNGGVPSSALT
ASHQNSAASSSAYTVKSGDTLSGIAATYGTTVSSLMSVNGLSNQNQLQVGQVLKISGTST
SAATASATSSSRSYTVQSGDSLSSIAARYGISIQQLMADNAIADADMIHVGEQLNIASSS
QNVATTQQVSQTTSTSSHSGYTHTVVAGESLYSIATANGMSAQRLAEINGIGINQTILPG
QTINI

Enzyme Prediction     

No EC number prediction in MGYG000003404_00305.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH73	33	165	5.7e-30	0.9765625

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG1705	FlgJ	1.79e-51	29	173	47	191	Flagellum-specific peptidoglycan hydrolase FlgJ [Cell wall/membrane/envelope biogenesis, Cell motility].
PRK06347	PRK06347	8.85e-47	20	365	146	523	1,4-beta-N-acetylmuramoylhydrolase.
PRK08581	PRK08581	2.21e-34	29	193	324	496	amidase domain-containing protein.
smart00047	LYZ2	4.77e-25	29	162	12	137	Lysozyme subfamily 2. Eubacterial enzymes distantly related to eukaryotic lysozymes.
PRK10783	mltD	3.47e-20	180	294	333	444	membrane-bound lytic murein transglycosylase D; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QDK70681.1	2.10e-128	1	365	1	353
AYF99639.1	2.97e-128	1	365	1	353
QSE63027.1	9.01e-127	1	365	1	361
AGV72470.1	7.31e-126	1	365	1	361
QRZ29333.1	2.95e-125	1	365	1	361

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3FI7_A	1.81e-29	29	170	34	183	CrystalStructure of the autolysin Auto (Lmo1076) from Listeria monocytogenes, catalytic domain [Listeria monocytogenes EGD-e]
5T1Q_A	9.36e-20	29	175	64	217	ChainA, N-acetylmuramoyl-L-alanine amidase domain-containing protein SAOUHSC_02979 [Staphylococcus aureus subsp. aureus NCTC 8325],5T1Q_B Chain B, N-acetylmuramoyl-L-alanine amidase domain-containing protein SAOUHSC_02979 [Staphylococcus aureus subsp. aureus NCTC 8325],5T1Q_C Chain C, N-acetylmuramoyl-L-alanine amidase domain-containing protein SAOUHSC_02979 [Staphylococcus aureus subsp. aureus NCTC 8325],5T1Q_D Chain D, N-acetylmuramoyl-L-alanine amidase domain-containing protein SAOUHSC_02979 [Staphylococcus aureus subsp. aureus NCTC 8325]
5DN5_A	1.43e-12	42	164	20	148	Structureof a C-terminally truncated glycoside hydrolase domain from Salmonella typhimurium FlgJ [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2],5DN5_B Structure of a C-terminally truncated glycoside hydrolase domain from Salmonella typhimurium FlgJ [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2],5DN5_C Structure of a C-terminally truncated glycoside hydrolase domain from Salmonella typhimurium FlgJ [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
5DN4_A	1.93e-12	42	164	20	148	Structureof the glycoside hydrolase domain from Salmonella typhimurium FlgJ [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
2ZYC_A	9.80e-11	36	168	13	157	ChainA, Peptidoglycan hydrolase FlgJ [Sphingomonas sp. A1]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P37710	1.30e-49	29	363	184	538	Autolysin OS=Enterococcus faecalis (strain ATCC 700802 / V583) OX=226185 GN=EF_0799 PE=1 SV=2
P39046	5.22e-41	21	363	57	453	Muramidase-2 OS=Enterococcus hirae (strain ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258 / NCTC 12367 / WDCM 00089 / R) OX=768486 GN=EHR_05900 PE=1 SV=1
A2RHZ5	5.37e-39	29	299	66	365	Probable N-acetylmuramidase OS=Lactococcus lactis subsp. cremoris (strain MG1363) OX=416870 GN=acmA PE=3 SV=1
P0C2T5	1.04e-38	29	365	66	436	Probable N-acetylmuramidase OS=Lactococcus lactis subsp. cremoris OX=1359 GN=acmA PE=3 SV=1
Q9CIT4	3.96e-37	29	299	66	367	Probable N-acetylmuramidase OS=Lactococcus lactis subsp. lactis (strain IL1403) OX=272623 GN=acmA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000271	0.999021	0.000207	0.000177	0.000152	0.000134

TMHMM  Annotations     

start	end
7	29