CAZyme Information: MGYG000003405_00530

Basic Information

• Species: Leuconostoc citreum
• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Leuconostoc; Leuconostoc citreum
• CAZyme ID: MGYG000003405_00530
• CAZy Family: GH70
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2822		312298.84	4.6626

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003405	1610057	MAG	United States	North America

• Gene Location: Start: 1841; End: 10309 Strand: +

Full Sequence      

MRDMRVICDRKKLYKSGKVLVTAGIFALMMFGVTTASVSANTIAVDTNHSRTSAQINKSA
VDKVNDDKTTLGAVKVVAVATTPATPVADKTVSAPAADKAVDTTSSTTPATDKAVDTTPT
TPAADKTVDTTPTTPAANKAVDTTPATAATDKAVDTPATPAADKLANTTPATDKAVATTP
ATPVANKAADTSSVHDQPLDTNVSTDKSANLVSTTQKSTDNQQVKSTETSHLQEINGKTY
FLDDNGQVKKNFTAIIDGKVLYFDKTSGELTANAPQVTKGLVNIDNAHNAAHDLTADNFT
NVDGYLTANSWYRPKDILKNGTTWTPTTAEDFRPLLMSWWPDKNTQVAYLQYMQSVGMLP
DDVKVSNDDNMSTLTDAAMTVQKNIESRIGVSGKTDWLKQDMNKLIDSLANWNIDSESKG
DDHLQGGALLYVNDDKTPNANSDYRLLNRTPTNQTGQITDPNKQGGYEMLLANDVDNSNP
VVQAEQLNWLHYMMNIGTIAQNDPTANFDGYRVDAVDNVDADLLQIAGDYFKAAYGTGKT
EANANNHISILEDWDNNDPAYIQAHGNNQLTMDFPAHLALKYALNMPLAAQSGLEPLINT
SLVKRGKDATENEAQPNYAFIRAHDSEVQTVIAQIIKDKINTKSEGLTGTPDEIKQAFNI
YNADQLKTDKEYTAYNIPASYAVLLTNKDTVPRVYYGDLFSDDGQYMSQKSPYYDAITSL
LKNRIKYVAGGQSMNMTYLHECFDPAKNETKPQGVLTSVRYGKGAMTADDLGNSDTRQQG
IGLVINNKPFLNLNDDEQIVLNMGAAHKNQAYRPLMLTTKSGLQIYDKDAGAPVVYTNDA
GQLIFKSDMVYGVSNPQVSGYFAAWVPVGASDSQDARTQSSQSETKDGDVYHSNAALDSN
VIYEGFSNFQAMPEKNDDFTNVKIAQNAKWFKDLGITSFELAPQYRSSTDNSFLDSVIQN
GYAFTDRYDVGYNTPTKYGTVDQLLDSLRALHAQGIQAINDWVPDQIYNLPGEQIVTAVR
TNGSGKYDYDSVINNTLYDSRTVGGGEYQEKFGGLFLDQLKKDYPSLFETKQISTNQPMN
PDVKIKEWSAKYFNGSNIQGRGAWYVLKDWATNQYFNVSSDNGFLPKQLLGEKTSTGFIT
ENGKTSFYSTSGYQAKDTFIQDGTNWYYFDNAGYMLTGKQNIHDKNYYFLPNGVELQDAY
LFDGNQEFYYNKAGEQVMNQYYQDSQNQWHYFFENGRMAIGLTEVPNADGTHVTQYFDAN
GVQIKGTAIKDQNNQLRYFDEATGNMVVNSWGELPDGSWLYLNDKGIAVTGNQKIDGEEY
YFNADGKQVKGNAIIDNNGDQRYYDGDKGVMAVNSWGELPDGSWLYLNDKGIAVTGRQVI
NNQVNFFGNDGKQIKDAFKLLSDGSWVYLDDKGQITTGAKVINGLNMFFDKDGHQIKGDA
STDANGKRHYYDKNDGHLVTNSWGELPDSSWLYLDEQGDAVTGQRVIDGKTRYFDEDGKQ
IKNSLKTLANGDKIYLDGDGVAATGLQHVGDKIMYFDEDGKQVVSKFISAKDGSRYYLNQ
DGVAAVGPSSINGQLLYFDQDGKQVKFNEVRNSDGTTNYYTGLTGEKLTQDFGELQDGSW
VYLDTQGNTVTGAQIINGQNLYFKADGQQVKGHAYTDQLGHMRFYDYDSGDMLSNRFEQI
TPGVWAYFGADGVAITGQHDINGQKLFFDETGYQVKGSQRTIDGTLYSFDSQTGNQKRVQ
TTLLPQAGHYITKNGNDWQYDTNGELAKGLRQDSNGKLRYFDLTTGIQAKGQFVTIGQET
YYFSKDHGDAQLLPTVTEGHYGTITLKQGQDTKTAWVYRDQNNTILKGLQNINGTLQFFD
PYTGEQLKGGVAKYDDKLFYFESGKGNLVSTVAGDYQDGHYISQDGQTRYADKQNQLVKG
LVTVNGALQYFDNVTGNQIKNQQVIVDGKTYYFDDKGNGEYLFTNTLDMSTNAFSAKNVA
FNHDSSSFDHTVDGFLTADTWYRPKSILANGTTWRDSTDKDMRPLITVWWPNKNVQVNYL
NFMKANGLLTTAAQYTLHSDQYDLNQAAQDVQVAIEKRIASEHGTEWLQKLLFESQNNNP
SFVKQQFIWNKDSEYHGGGDAWFQGGYLKYGNNPLTPTTNSDYRQPGNAFDFLLANDVDN
SNPVVQAENLNWLHYLMNFGTITAGQDDANFDSIRIDAVDFIHNDTIQRTYDYLRDAYQV
QQSEAKANQHISLVEAGLDAGTSTIHNDALIESNLREAATLSLTNEPGKNKPLTNMLQDV
DGGTLIADHTQNNTENQATPNYSIIHAHDKDVQEKVGAAITDATGADWTNFTDEQLKAGL
ELFYKDQRATNKKYNSYNIPSVYALMLTNKDTVPRMYYGDMYQDDGQYMANKSIYYDALV
SLMTARKSYVSGGQTMSVDNHGLLKSVRFGKDAMTANDLGTSATRTEGIGVIIGNDSKLQ
LNDSDKVTLDMGAAHKNQKYRAVILTTRDGLATFNSDQAPTAWTNDQGTLTFSNQEINGQ
DNTQIRGVANPQVSGYLAVWVPVGASDNQDARTAATTTENHDGKVLHSNAALDSNLIYEG
FSNFQPKATTHDELTNVVIAKNADVFNNWGITSFEMAPQYRSSGDHTFLDSTIDNGYAFT
DRYDLGFNTPTKYGTDGDLRAAIQALHHANMQVMADVVDNQVYNLPGKEVVSATRAGVYG
NDDATGFGTQLYVTNSVGGGQYQEKYAGQYLEALKAKYPDLFEGKAYDYWYKNYANDGSN
PYYTLSHGDRESIPADVAIKQWSAKYMNGTNVLGNGMGYVLKDWHNGQYFKLDGDKSTLP
QI

Enzyme Prediction     

EC	2.4.1.5	2.4.1.-	2.4.1.140

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH70	335	1141	0	0.9975093399750934
GH70	2025	2821	0	0.9800747198007472

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam02324	Glyco_hydro_70	0.0	335	1144	1	804	Glycosyl hydrolase family 70. Members of this family belong to glycosyl hydrolase family 70 Glucosyltransferases or sucrose 6-glycosyl transferases (GTF-S) catalyze the transfer of D-glucopyramnosyl units from sucrose onto acceptor molecules, EC:2.4.1.5. This family roughly corresponds to the N-terminal catalytic domain of the enzyme. Members of this family also contain the Putative cell wall binding domain pfam01473, which corresponds with the C-terminal glucan-binding domain.
pfam02324	Glyco_hydro_70	0.0	2025	2821	1	788	Glycosyl hydrolase family 70. Members of this family belong to glycosyl hydrolase family 70 Glucosyltransferases or sucrose 6-glycosyl transferases (GTF-S) catalyze the transfer of D-glucopyramnosyl units from sucrose onto acceptor molecules, EC:2.4.1.5. This family roughly corresponds to the N-terminal catalytic domain of the enzyme. Members of this family also contain the Putative cell wall binding domain pfam01473, which corresponds with the C-terminal glucan-binding domain.
NF033930	pneumo_PspA	5.11e-18	1136	1407	443	660	pneumococcal surface protein A. The pneumococcal surface protein proteins, found in Streptococcus pneumoniae, are repetitive, with patterns of localized high sequence identity across pairs of proteins given different specific names that recombination may be presumed. This protein, PspA, has an N-terminal region that lacks a cross-wall-targeting YSIRK type extended signal peptide, in contrast to the closely related choline-binding protein CbpA which has a similar C-terminus but a YSIRK-containing region at the N-terminus.
TIGR04035	glucan_65_rpt	1.74e-17	1301	1362	2	62	glucan-binding repeat. This model describes a region of about 63 amino acids that is composed of three repeats of a more broadly distributed family of shorter repeats modeled by pfam01473. While the shorter repeats are often associated with choline binding (and therefore with cell wall binding), the longer repeat described here represents a subgroup of repeat sequences associated with glucan binding, as found in a number glycosylhydrolases. Shah, et al. describe a repeat consensus, WYYFDANGKAVTGAQTINGQTLYFDQDGKQVKG, that corresponds to half of the repeat as modeled here and one and a half copies of the repeat as modeled by pfam01473.
TIGR04035	glucan_65_rpt	2.31e-16	1408	1468	2	61	glucan-binding repeat. This model describes a region of about 63 amino acids that is composed of three repeats of a more broadly distributed family of shorter repeats modeled by pfam01473. While the shorter repeats are often associated with choline binding (and therefore with cell wall binding), the longer repeat described here represents a subgroup of repeat sequences associated with glucan binding, as found in a number glycosylhydrolases. Shah, et al. describe a repeat consensus, WYYFDANGKAVTGAQTINGQTLYFDQDGKQVKG, that corresponds to half of the repeat as modeled here and one and a half copies of the repeat as modeled by pfam01473.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QQE97705.1	0.0	1	2822	1	2836
CDX66820.1	0.0	1	2822	1	2835
ACK10800.1	0.0	1	2822	1	2835
CAD22883.1	0.0	1	2822	1	2835
CCF26454.1	0.0	1	2822	1	2824

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4TVD_A	0.0	1746	2822	2	1078	N-terminallytruncated dextransucrase DSR-E from Leuconostoc mesenteroides NRRL B-1299 in complex with D-glucose [Leuconostoc mesenteroides subsp. mesenteroides]
3TTO_A	0.0	1746	2822	2	1078	Crystalstructure of Leuconostoc mesenteroides NRRL B-1299 N-terminally truncated dextransucrase DSR-E in triclinic form [Leuconostoc mesenteroides],3TTO_B Crystal structure of Leuconostoc mesenteroides NRRL B-1299 N-terminally truncated dextransucrase DSR-E in triclinic form [Leuconostoc mesenteroides],3TTO_C Crystal structure of Leuconostoc mesenteroides NRRL B-1299 N-terminally truncated dextransucrase DSR-E in triclinic form [Leuconostoc mesenteroides],3TTO_D Crystal structure of Leuconostoc mesenteroides NRRL B-1299 N-terminally truncated dextransucrase DSR-E in triclinic form [Leuconostoc mesenteroides],3TTQ_A Crystal structure of Leuconostoc mesenteroides NRRL B-1299 N-terminally truncated dextransucrase DSR-E in orthorhombic apo-form at 1.9 angstrom resolution [Leuconostoc mesenteroides]
4TTU_A	0.0	1746	2822	2	1078	N-terminallytruncated dextransucrase DSR-E from Leuconostoc mesenteroides NRRL B-1299 in complex with isomaltotriose [Leuconostoc mesenteroides],4TVC_A N-terminally truncated dextransucrase DSR-E from Leuconostoc mesenteroides NRRL B-1299 in complex with gluco-oligosaccharides [Leuconostoc mesenteroides]
3AIB_A	4.72e-298	288	1130	5	842	CrystalStructure of Glucansucrase [Streptococcus mutans],3AIB_B Crystal Structure of Glucansucrase [Streptococcus mutans],3AIB_C Crystal Structure of Glucansucrase [Streptococcus mutans],3AIB_D Crystal Structure of Glucansucrase [Streptococcus mutans],3AIB_E Crystal Structure of Glucansucrase [Streptococcus mutans],3AIB_F Crystal Structure of Glucansucrase [Streptococcus mutans],3AIB_G Crystal Structure of Glucansucrase [Streptococcus mutans],3AIB_H Crystal Structure of Glucansucrase [Streptococcus mutans],3AIC_A Crystal Structure of Glucansucrase from Streptococcus mutans [Streptococcus mutans],3AIC_B Crystal Structure of Glucansucrase from Streptococcus mutans [Streptococcus mutans],3AIC_C Crystal Structure of Glucansucrase from Streptococcus mutans [Streptococcus mutans],3AIC_D Crystal Structure of Glucansucrase from Streptococcus mutans [Streptococcus mutans],3AIC_E Crystal Structure of Glucansucrase from Streptococcus mutans [Streptococcus mutans],3AIC_F Crystal Structure of Glucansucrase from Streptococcus mutans [Streptococcus mutans],3AIC_G Crystal Structure of Glucansucrase from Streptococcus mutans [Streptococcus mutans],3AIC_H Crystal Structure of Glucansucrase from Streptococcus mutans [Streptococcus mutans],3AIE_A Crystal Structure of glucansucrase from Streptococcus mutans [Streptococcus mutans],3AIE_B Crystal Structure of glucansucrase from Streptococcus mutans [Streptococcus mutans],3AIE_C Crystal Structure of glucansucrase from Streptococcus mutans [Streptococcus mutans],3AIE_D Crystal Structure of glucansucrase from Streptococcus mutans [Streptococcus mutans],3AIE_E Crystal Structure of glucansucrase from Streptococcus mutans [Streptococcus mutans],3AIE_F Crystal Structure of glucansucrase from Streptococcus mutans [Streptococcus mutans],3AIE_G Crystal Structure of glucansucrase from Streptococcus mutans [Streptococcus mutans],3AIE_H Crystal Structure of glucansucrase from Streptococcus mutans [Streptococcus mutans]
5LFC_A	5.32e-291	249	1188	226	1220	Crystalstructure of Leuconostoc citreum NRRL B-1299 N-terminally truncated dextransucrase DSR-M [Leuconostoc citreum],5LFC_B Crystal structure of Leuconostoc citreum NRRL B-1299 N-terminally truncated dextransucrase DSR-M [Leuconostoc citreum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P08987	0.0	231	1673	161	1473	Glucosyltransferase-I OS=Streptococcus mutans serotype c (strain ATCC 700610 / UA159) OX=210007 GN=gtfB PE=3 SV=3
P11001	0.0	230	1806	158	1588	Glucosyltransferase-I OS=Streptococcus downei OX=1317 GN=gtfI PE=3 SV=1
P13470	0.0	231	1496	186	1408	Glucosyltransferase-SI OS=Streptococcus mutans serotype c (strain ATCC 700610 / UA159) OX=210007 GN=gtfC PE=1 SV=2
P27470	0.0	230	1806	152	1583	Glucosyltransferase-I OS=Streptococcus downei OX=1317 PE=3 SV=1
P49331	0.0	231	1494	173	1408	Glucosyltransferase-S OS=Streptococcus mutans serotype c (strain ATCC 700610 / UA159) OX=210007 GN=gtfD PE=3 SV=3

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000490	0.998781	0.000226	0.000159	0.000145	0.000139

TMHMM  Annotations     

start	end
21	43