CAZyme Information: MGYG000003422_00218

Basic Information

• Species: UBA1712 sp900766815
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; UBA1712; UBA1712 sp900766815
• CAZyme ID: MGYG000003422_00218
• CAZy Family: CBM48
• CAZyme Description: 1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
699		79430.99	5.3069

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003422	1683805	MAG	Fiji	Oceania

• Gene Location: Start: 8280; End: 10379 Strand: +

Full Sequence      

MKDKLYKMMDWPGIEAIVYGEESSPQNLLGRHSTATNTLYQCFFPNAKEVKLCIESEGRD
AKEYVMEMADEEGFYAYALLGKVKDDYSYIVTDINGVKTTVKDPYYYSAMLKPEDSRKFN
SGTETEGYKYMGAHVETIDGVKGVAFRVWAPNAMRVSVLGDFNAWNGKANPMMKDEESGI
FTLFIPGLTGGCEYKYEIKGHGGLVCIKLDPYSYECNDGKSVVPSNARFKWEDKEYFDVR
SGAKLDASPVNIYETEIGRITDKKGELGKSAITDLIGYLKKMGYNYLKLTMKGDDSYMLP
AGVGAASVKNLVNALHKENIGILYTWNPSFFAVTNEGMSKFDGTYLYGHMDMRKRYNNAF
GGFYYNYGREEVLSYLYSAANYWMKEFHMDGFHIEGLSSMLYLDYAKYDGEWIANIYGGH
ENLEAIEFIKELNLKLHKAYPYVIMTTREESAFPKVTSSNKDGGLGFDFVYNNGFTEDYL
SFIKGDRAFEELHMLTDHFAYEYSENYISALSKETVLAANDYDYVRVKNGARFVDYVPVP
DENKGTVVRATLAFFMAHPGKKLMYCGQDDENLSLALGQLYNTEPALYSLDNSPYGIEWI
NNLDKGDGVLSFIRKSEKAEDMIIVVCNFSCKDINNHKLGVMRPGKYKQIFNSEDKKFGG
KAATAVKPKETVEEFYEGRYHTITIKIPAMSVSYYKYVE

Enzyme Prediction     

No EC number prediction in MGYG000003422_00218.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	307	569	2.7e-67	0.8438538205980066
CBM48	131	215	3.8e-18	0.881578947368421

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK05402	PRK05402	7.26e-179	14	696	6	722	1,4-alpha-glucan branching protein GlgB.
PRK12313	PRK12313	3.43e-164	110	698	6	628	1,4-alpha-glucan branching protein GlgB.
COG0296	GlgB	1.04e-128	106	697	1	628	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].
PRK14705	PRK14705	1.93e-110	86	696	578	1221	glycogen branching enzyme; Provisional
PRK12568	PRK12568	1.51e-101	101	694	97	725	glycogen branching enzyme; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AWY97839.1	1.13e-163	1	699	1	734
QNM09059.1	8.64e-161	1	699	1	732
QQQ92601.1	3.08e-158	1	699	1	734
ASU27851.1	3.08e-158	1	699	1	734
ANU75041.1	3.08e-158	1	699	1	734

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GR2_A	1.42e-107	14	692	30	768	Crystalstructure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GR4_A Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GQZ_A	5.46e-107	14	692	30	768	Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQW_A	7.64e-107	14	692	30	768	Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GQU_A	7.64e-107	14	692	30	768	Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR5_A	7.64e-107	14	692	30	768	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q21M30	4.30e-122	14	691	9	721	1,4-alpha-glucan branching enzyme GlgB OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) OX=203122 GN=glgB PE=3 SV=1
Q1AZ86	5.01e-122	14	691	7	713	1,4-alpha-glucan branching enzyme GlgB OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1) OX=266117 GN=glgB PE=3 SV=1
Q081Q4	5.90e-114	16	693	15	730	1,4-alpha-glucan branching enzyme GlgB OS=Shewanella frigidimarina (strain NCIMB 400) OX=318167 GN=glgB PE=3 SV=1
Q8EGU7	6.05e-114	16	660	15	697	1,4-alpha-glucan branching enzyme GlgB OS=Shewanella oneidensis (strain MR-1) OX=211586 GN=glgB PE=3 SV=2
P52981	8.63e-113	29	692	30	747	1,4-alpha-glucan branching enzyme GlgB OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000048	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003422_00218.