CAZyme Information: MGYG000003427_02211

Basic Information

• Species: HGM12587 sp900766915
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; HGM12587; HGM12587 sp900766915
• CAZyme ID: MGYG000003427_02211
• CAZy Family: GH2
• CAZyme Description: Exo-beta-D-glucosaminidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
821	MGYG000003427_294|CGC1	95876.62	5.1445

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003427	3377258	MAG	Fiji	Oceania

• Gene Location: Start: 6061; End: 8526 Strand: -

Full Sequence      

MTILELNGIWELRDENGKVLCPVKVPGSVISGLYAAGKIEHPYVGENEYRIRSLFEKDYE
FVRFFEMNAEILRNREIQLVCEGLDTLTQIFINDVEIATTNNMHRTYSFSVKDYIKEGQN
EIRIVFHSVLQYIRNYQYRENKQIHYVTCGAMEGNQLIRKAHSMFGWDWGPQLVDAGIWR
DIYLVASSGTQIEDTRIRQVHEKDGTVRIKIEITLSEETKKQVEASLEEPESRKHLPAEV
RKVAPCLYEAEIPVKNPKLWWPNGYGSQPLYRLKLSIGQETVLEKTIGLRTITVSQKEDE
WGNEFVFLVNGEKIFARGGNYIPEDAVYPWISKEKIAYLLKSCVRANFNCIRVWGGGYYP
SDEFYDLCDRYGLIVWQDLMYACNVYDVTEEFAETVRWETIDNVRRIRHHASLGLWCGNN
EIESAWHHWGDFQKESPYLRADYIKLFEEILPKAVRSVDDQTFYWPSSPSSGGCFDEPDS
DKRGDVHYWDVWHGQKPFSDYRKYYFRFCSEFGFQSFPCLKTIAMFAPKEDWNIFSKVME
SHQKNDSANGKILYYLSENFRYPKDFKSLLYVSQILQGIAIKSGVEHWRRNRGRCMGTLY
WQINDNWPVASWASIDYYGRWKALHYMARKFYAPIEVSICKCEHEISVFLENETHEEIIC
REAFLRIRDTHFGIVYECSTTAKVAAFNADKLIACKMESDWLERADIYIEAEVLLEDGTI
LIDTDTFIPYKHLELPRVHIDTAVEETKDYFEITISGDGFAPFVELDFEDADVIFSDNFF
TISNEKKIRTTIQKADIQNGSFADANDLKKRLAITTLVDTF

Enzyme Prediction     

No EC number prediction in MGYG000003427_02211.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	4	655	4.9e-107	0.6356382978723404

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	3.08e-106	6	780	15	773	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam17753	Ig_mannosidase	8.45e-16	737	819	1	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
PRK10340	ebgA	7.09e-13	74	421	125	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam00703	Glyco_hydro_2	8.74e-10	192	290	3	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
PRK09525	lacZ	1.05e-08	177	375	208	410	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBL20579.1	0.0	1	817	1	838
QCU02115.1	0.0	1	817	1	824
CBL13897.1	1.18e-277	6	791	16	834
CBL08662.1	3.75e-277	6	791	9	827
VCV23589.1	1.51e-276	6	791	9	827

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VJX_A	3.98e-154	3	806	8	830	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	4.21e-154	3	806	10	832	Structureof a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	4.33e-154	3	806	10	832	ChainA, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	1.14e-153	3	806	8	830	Structureof the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]
2VQU_A	4.75e-152	3	806	8	830	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQU_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O00462	5.73e-107	6	821	25	879	Beta-mannosidase OS=Homo sapiens OX=9606 GN=MANBA PE=1 SV=3
Q29444	4.41e-104	4	821	23	879	Beta-mannosidase OS=Bos taurus OX=9913 GN=MANBA PE=1 SV=1
Q0CCA0	5.51e-104	11	796	17	832	Beta-mannosidase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndB PE=3 SV=2
Q95327	1.69e-102	4	821	23	879	Beta-mannosidase OS=Capra hircus OX=9925 GN=MANBA PE=1 SV=1
A1CGA8	2.22e-102	10	648	13	669	Beta-mannosidase B OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=mndB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000049	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003427_02211.