CAZyme Information: MGYG000003451_01786

Basic Information

• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Burkholderiales; Burkholderiaceae; Oxalobacter
• CAZyme ID: MGYG000003451_01786
• CAZy Family: GH103
• CAZyme Description: Membrane-bound lytic murein transglycosylase B

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
343		37512.08	4.6439

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003451	1832891	MAG	Fiji	Oceania

• Gene Location: Start: 2415; End: 3446 Strand: -

Full Sequence      

MPDDSDSISSPSSSVSIPSPVTTQPDADTSFETAKSEFIEAFSDKHAFDKAQLITLFEQV
HRNPTVIRLVTPSGKAKAKNWQNYRKNVIDPARISAGVRFWNQYEDALDRATQTYGVPSD
IIVAIIGVESVYGRYTGNFRIIDALSTLAFSYPETANQAARKRFFRKELENLLLLAKETG
TDPANLYGSYAGAIGYPQFMPGSIRQFAVDFDGDGAIDLENSPVDAIGSIANFLVSHGWK
AGLPIVFPVQTQHGCTIPPSLLDQGLAATLSLSQLENGCIIPEKDVPDNISFGLIDLPNG
TAPADYWIGTDNFFAITHYNRSYFYAMSVVLLAQEIAHARNGE

Enzyme Prediction     

No EC number prediction in MGYG000003451_01786.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH103	43	337	1.6e-90	0.9593220338983051

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
TIGR02282	MltB	1.56e-124	37	336	2	290	lytic murein transglycosylase B. This family consists of lytic murein transglycosylases (murein hydrolases) in the family of MltB, which is a membrane-bound lipoprotein in Escherichia coli. The N-terminal lipoprotein modification motif is conserved in about half the members of this family. The term Slt35 describes a naturally occurring soluble fragment of MltB. Members of this family never contain the putative peptidoglycan binding domain described by pfam01471, which is associated with several classes of bacterial cell wall lytic enzymes. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
pfam13406	SLT_2	4.53e-115	30	334	1	292	Transglycosylase SLT domain. This family is related to the SLT domain pfam01464.
PRK10760	PRK10760	8.33e-105	22	342	47	359	murein hydrolase B; Provisional
COG2951	MltB	8.57e-94	45	340	54	339	Membrane-bound lytic murein transglycosylase B [Cell wall/membrane/envelope biogenesis].
TIGR02283	MltB_2	2.46e-62	83	338	53	300	lytic murein transglycosylase. Members of this family are closely related to the MltB family lytic murein transglycosylases described by TIGR02282 and are likewise all proteobacterial, although that family and this one form clearly distinct clades. Several species have one member of each family. Many members of this family (unlike the MltB family) contain an additional C-terminal domain, a putative peptidoglycan binding domain (pfam01471), not included in region described by this model. Many sequences appear to contain N-terminal lipoprotein attachment sites, as does E. coli MltB in TIGR02282. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QDX34016.1	7.61e-131	32	342	62	372
ARQ77449.1	7.61e-131	32	342	62	372
ARQ45141.1	7.61e-131	32	342	62	372
AMP06889.1	3.55e-119	36	340	67	374
AIY43440.1	5.74e-119	36	340	50	357

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5O8X_A	2.71e-79	36	340	9	303	TheX-ray Structure of Catenated Lytic Transglycosylase SltB1 from Pseudomonas aeruginosa [Pseudomonas aeruginosa],5O8X_B The X-ray Structure of Catenated Lytic Transglycosylase SltB1 from Pseudomonas aeruginosa [Pseudomonas aeruginosa]
4ANR_A	4.58e-79	36	340	26	320	Crystalstructure of soluble lytic Transglycosylase SltB1 from Pseudomonas aeruginosa [Pseudomonas aeruginosa PAO1]
1LTM_A	1.88e-77	37	340	21	318	AcceleratedX-ray Structure Elucidation Of A 36 Kda Muramidase/transglycosylase Using Warp [Escherichia coli]
1D0K_A	2.00e-77	37	340	23	320	ChainA, 35KD SOLUBLE LYTIC TRANSGLYCOSYLASE [Escherichia coli],1D0L_A Chain A, 35KD SOLUBLE LYTIC TRANSGLYCOSYLASE [Escherichia coli],1D0M_A Chain A, 35KD SOLUBLE LYTIC TRANSGLYCOSYLASE [Escherichia coli],1QDR_A Chain A, LYTIC MUREIN TRANSGLYCOSYLASE B [Escherichia coli],1QDT_A Chain A, LYTIC MUREIN TRANSGLYCOSYLASE B [Escherichia coli],1QUS_A Chain A, LYTIC MUREIN TRANSGLYCOSYLASE B [Escherichia coli],1QUT_A Chain A, LYTIC MUREIN TRANSGLYCOSYLASE B [Escherichia coli]
5ANZ_A	2.89e-32	11	333	30	340	CrystalStructure of SltB3 from Pseudomonas aeruginosa. [Pseudomonas aeruginosa],5AO7_A Crystal Structure of SltB3 from Pseudomonas aeruginosa in complex with NAG-anhNAM-pentapeptide [Pseudomonas aeruginosa]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P41052	3.52e-76	37	340	62	359	Membrane-bound lytic murein transglycosylase B OS=Escherichia coli (strain K12) OX=83333 GN=mltB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000059	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003451_01786.