CAZyme Information: MGYG000003461_00245

Basic Information

• Species: Prevotella sp900553595
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella; Prevotella sp900553595
• CAZyme ID: MGYG000003461_00245
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
884		99630.65	6.736

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003461	2310521	MAG	Fiji	Oceania

• Gene Location: Start: 873; End: 3527 Strand: -

Full Sequence      

MLLLLLFLSCITAGAAQQPEFSTAGFFEIPNSGREVFSMNPAWRFHKGAIDGAETRSFDD
SGWAVVSLPHGIDYLPTEASGCTNYQGEVWYRKHFCPEARLKGKKLFMHFEAIMGKSRVY
VNGRLLAEHFGGYLPVVVDITDAIDWQGDNVVSVWTDNSDDPYYPPGKAQDVLDFTYFGG
IYRDCWLVAHDRMHITDPNYENVVAGGGLFVSYDKVSEASALVRLQLHMRNERSAAFAGV
AEFELVRPDGTKAATINRKIAVKAGRTATVRSSVVLEKPMLWSPEEPQLYNLFVRIRDAK
GNVIDGYRRRIGVRSVEFKGKDGFWLNGKLYEDPLIGANRHQDFAVVGNAVPNSLHWRDA
KKLRDAGLKVIRNAHCPQDPAFMDACDELGLFVLDNAPGWQFWNDAPVFAQRVYSDIRNL
VRRDRNHACLWMFEPILNETWYPADFAKTTQNIVKEEYPYPYCYSGCDATARGHEYYSIQ
FTHPVNGAGGAFSADVKDDRITCFTREWGDNVDDWSSHNSPSRVARNWGEVPMLVQAQHY
AKPSYHYTSYDALYRTSRQHIGGCLWHSFDHQRGYHPDPFYGGLMDVFRQPKYSYYMFKS
QRSAVKEDRPFETGPMVYIAHEMTPFSPKDVTVYSNCDEVRLTYNKGGKVFSYHKPAQHE
GMPSPVITFKDVYDFMIDKEMSRDKRQDDVWLLAEGIIDGKVVATHKVCPARRPEKIMLW
IDNEGEQLRADGSDAVVVVAAVADRNGNIKRLNNDYIKFSIEGEGRILGGADVLANPAPV
KWGTAPVLVQSTLKPGKIRLTASVLFEGSQMPVSGSIEFESVPAAKPLVFSYPAAALIPS
FTGAPLVAAKKSATDLEAEQKQRDANNLRLKEVEQQQEDFGEKK

Enzyme Prediction     

No EC number prediction in MGYG000003461_00245.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	33	605	1.4e-86	0.6156914893617021

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.16e-45	28	444	4	411	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	1.39e-34	34	439	10	417	beta-D-glucuronidase; Provisional
PRK10340	ebgA	2.42e-32	74	432	103	443	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	1.92e-16	74	429	114	453	beta-galactosidase.
pfam02836	Glyco_hydro_2_C	2.45e-16	316	447	1	143	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT85817.1	0.0	2	884	9	892
QJR74048.1	0.0	2	884	9	892
QJR69714.1	0.0	2	884	9	892
QJR65452.1	0.0	2	884	9	892
AII65553.1	0.0	2	884	9	892

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5UJ6_A	5.52e-60	20	457	11	430	CrystalStructure of Bacteroides Uniformis beta-glucuronidase [Bacteroides uniformis str. 3978 T3 ii],5UJ6_B Crystal Structure of Bacteroides Uniformis beta-glucuronidase [Bacteroides uniformis str. 3978 T3 ii],6NZG_A Bacteroides uniformis beta-glucuronidase 2 covalently bound to cyclophellitol-6-carboxylate aziridine [Bacteroides uniformis],6NZG_B Bacteroides uniformis beta-glucuronidase 2 covalently bound to cyclophellitol-6-carboxylate aziridine [Bacteroides uniformis]
6D50_A	6.71e-59	34	457	33	438	Bacteroidesuniforms beta-glucuronidase 2 bound to D-glucaro-1,5-lactone [Bacteroides uniformis str. 3978 T3 ii],6D50_B Bacteroides uniforms beta-glucuronidase 2 bound to D-glucaro-1,5-lactone [Bacteroides uniformis str. 3978 T3 ii]
6D8G_A	1.84e-57	34	457	33	438	D341AD367A calcium binding mutant of Bacteroides uniformis beta-glucuronidase 2 [Bacteroides uniformis str. 3978 T3 ii],6D8G_B D341A D367A calcium binding mutant of Bacteroides uniformis beta-glucuronidase 2 [Bacteroides uniformis str. 3978 T3 ii]
5T98_A	2.75e-56	38	803	29	807	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]
3FN9_A	6.61e-51	34	645	5	616	Crystalstructure of putative beta-galactosidase from bacteroides fragilis [Bacteroides fragilis NCTC 9343],3FN9_B Crystal structure of putative beta-galactosidase from bacteroides fragilis [Bacteroides fragilis NCTC 9343],3FN9_C Crystal structure of putative beta-galactosidase from bacteroides fragilis [Bacteroides fragilis NCTC 9343],3FN9_D Crystal structure of putative beta-galactosidase from bacteroides fragilis [Bacteroides fragilis NCTC 9343]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P77989	7.17e-98	33	814	2	735	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
P26257	8.94e-57	34	773	2	710	Beta-galactosidase OS=Thermoanaerobacterium thermosulfurigenes OX=33950 GN=lacZ PE=1 SV=1
T2KN75	6.17e-49	29	802	21	781	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1
A7LXS9	1.07e-46	32	803	40	835	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KPJ7	1.68e-44	22	645	39	668	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000446	0.998842	0.000185	0.000183	0.000159	0.000163

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003461_00245.