CAZyme Information: MGYG000003461_00866

Basic Information

• Species: Prevotella sp900553595
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella; Prevotella sp900553595
• CAZyme ID: MGYG000003461_00866
• CAZy Family: GH85
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1246		136466.5	7.167

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003461	2310521	MAG	Fiji	Oceania

• Gene Location: Start: 93; End: 3833 Strand: +

Full Sequence      

MKKSYKKHALLTAGLAFCVLGASAQQIRPGYVTLGENTGSPQFHTLLNKWTPGSKVSEDD
NFFISRVKPHARFRNQATQVRNTLTADNDKKLLAWVPINNPDYNALPDGVFDSEVFSMWS
YVTHWGDWSAPLGRIPGAFLDVAHKNGVPVSGVAGIPNAGLSGDYYYMLAGLGSADVDKA
AKFFRYYGIDGLGYNSEFNNGSTVMPKLRTFHINLNKKMKETNPLFENIWYDGTNDYGTI
QFDQGLAGHNAKNFGDANNSCFSLFLNYNWNKTSLLSKSVAKAESLKRSPLDLYAGVNMQ
GGQPKYSSWTLLKDYPISIGLWGAHTYNMFWESRGELGSDPAVKQNAYMLRTERYFTGGT
RNPANCPAVDDVQAYNAYNYTWHGMSSFMTARSAMSWNLAEEPLITHFNLGNGKFFNLNG
VRQHDKSWYNIGVQDYLPTWRWWFATKLLGRNAADVPRHGLDASFTWDDAYFGGSTVRIS
GSVPEEYLHLFKTQYSLKKGDVITFKYKLAAGTSDVELLLSAVGSEDKPTAYSLCKQTQE
ADDEEWVTRTFTVGSDFDGKDLALVALHFKNATNINLLLGEFSIVRGTYATPAKPQLESA
KLLYNCKDGMDGKIIFNMPNNKAAGEPCYNLDVKTSHFRLYAQQEGHDKVLMGTTTSWAG
LYYAIPVPGDVNGKMRLGVSAVSLDEKSESEITWSDYMAPATYVYNDDVKIDKNVIKPGE
EFTISYVDKNHPAAEWQLADTSGKVLRSGSGNSWRVEGLSDIGSYNLIVTGNQNQYDSEG
NFTGMEKTTRQMNSFVQVSGEAVGAVPEIYSLTANGKNENVSVDVAEKIEMAYTGRNADG
LSSQGVDLKEKHFGVKCSDIGVVGAKPFSLSFWLKLNKLADGVTQFISVTSKPDGWPKSE
WGWLWSTFDVKGNIECFTFRGIDGGPELQYKLEKANLPVGSWVHLALVFDYNSNGNFQSD
IYVNGKKLKVLSTNRTGTTAPGYQSGIYKITNGMVLAIGGDAFGRGGIDGIVDNVAVWNK
AMTADEVTLSMGDIDKKNLPAGIAAFWDFEEQSGTAKAFKAVGTKAGVEGGVYDEQPSTY
NVKWLDPAYSSGCPFLKGTAYKVQTLPSWKAAHGRVADAKGNGEVGSATVTYAKGGDYTV
TLTLANSIASAQRTFSVIKVSASGIGTTEAAEVKTYTVGENAVVEFAEAGSYEVGVYDTA
GVMQARKAATLNAGNVMNIHLAKAGIYVLTVKKDGKPVRTVKLVRK

Enzyme Prediction     

No EC number prediction in MGYG000003461_00866.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH85	110	416	2e-57	0.946031746031746

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG4724	COG4724	8.61e-46	49	584	41	549	Endo-beta-N-acetylglucosaminidase D [Carbohydrate transport and metabolism].
cd06547	GH85_ENGase	4.29e-38	110	443	21	334	Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the protein and N-acetylglucosamine 2 forms the reducing end of the released glycan. ENGase is a key enzyme in the processing of free oligosaccharides in the cytosol of eukaryotes. Oligosaccharides formed in the lumen of the endoplasmic reticulum are transported into the cytosol where they are catabolized by cytosolic ENGases and other enzymes, possibly to maximize the reutilization of the component sugars. ENGases have an eight-stranded alpha/beta barrel topology and are classified as a family 85 glycosyl hydrolase (GH85) domain. The GH85 ENGases are sequence-similar to the family 18 glycosyl hydrolases, also known as GH18 chitinases. An ENGase-like protein is also found in bacteria and is included in this alignment model.
pfam03644	Glyco_hydro_85	3.94e-29	111	413	5	291	Glycosyl hydrolase family 85. Family of endo-beta-N-acetylglucosaminidases. These enzymes work on a broad spectrum of substrates.
pfam13385	Laminin_G_3	2.23e-16	862	1027	13	150	Concanavalin A-like lectin/glucanases superfamily. This domain belongs to the Concanavalin A-like lectin/glucanases superfamily.
smart00560	LamGL	7.64e-06	868	1025	3	133	LamG-like jellyroll fold domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QFQ13703.1	0.0	4	1246	2	1247
EFC71164.1	0.0	6	1246	4	1247
QUB60779.1	0.0	10	1246	6	1250
QUB57152.1	0.0	10	1246	6	1244
QUB59112.1	0.0	10	1246	6	1244

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VTF_A	3.60e-34	43	683	17	608	X-raycrystal structure of the Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae E173Q mutant reveals a TIM barrel catalytic domain and two ancillary domains [Glutamicibacter protophormiae],2VTF_B X-ray crystal structure of the Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae E173Q mutant reveals a TIM barrel catalytic domain and two ancillary domains [Glutamicibacter protophormiae]
3FHA_A	6.12e-34	43	683	12	603	ChainA, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_B Chain B, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_C Chain C, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_D Chain D, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae]
3FHQ_A	6.12e-34	43	683	12	603	ChainA, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_B Chain B, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_D Chain D, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_F Chain F, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001382	0.993443	0.004191	0.000349	0.000308	0.000290

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003461_00866.