CAZyme Information: MGYG000003489_02080

Basic Information

• Species: Prevotellamassilia sp900768625
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotellamassilia; Prevotellamassilia sp900768625
• CAZyme ID: MGYG000003489_02080
• CAZy Family: GH16
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1129	MGYG000003489_330|CGC1	124517.13	5.609

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003489	2843857	MAG	Fiji	Oceania

• Gene Location: Start: 38955; End: 42344 Strand: -

Full Sequence      

MNKNKYICLAGLLFPLSLMAQTASDKVLCDFETTDSYKSVGVYDTWANSPFRTKKLTGNA
QVVKNHLTEVDPVLGEAPNSSSKILGVQRSRFGSNTFGALVELKEPFALKKTTQYVHVKI
YTPKGGTVMLIGLGNRDDRPHQSALTEQFWSTSTSKVADDRWCDAVFPVSGSNGITIRNL
LVVVDRNSPHNLTADYAAYIDDIVLSSQATPFFTSTTYPINYDENTAHSRGTNRYMAGVT
FSSSDGSQTLDADQSAINRLYIKKMDNAFLAKPGDKVTAGFIKGAMSWMGGYVYLDKDNN
GAFDVNYDDNGVNEAKDLMSYSMYKDKNSEGAAVSGEPALNPPAFTIPADLKPGFYRMRY
KVDWDNVDPGGNPGPANKITDNGGMILDTRVNIHGELVNLSRTTDELGGGGLNGDILMAD
GSAVTGQQVPFGKDLKVKMQPAPGFRFSHVVVRHGYNLEGDSLVNDNRQWDEKIFYARLF
RDNEFTIPGKYIDGDVRFIPYFSSTSGQEVGGDEYAINFDEELEMKDPESNVLTSLKLNC
SGGDATTLTVKNLNSTKVYRNLTPKELRVRPGDTVTPSVVYKGSNKAMNAYFYLDMNMDG
KFFSEVETDGKVSNNSELLSYSFYNGRNSNGTTTSAENAGCGLPIFQIPEDLPTGLYRAR
LKLDVNNIDPAGFWKEGESGGIDETGGYVVDFLVNVHNDNVNLEVNGIGGNIVGQSNKGI
PTTTKFGTVYYVFPQAPVAGIQLDRMVVRHGYNLDGEQYIHGNRQWSEYEVKNPKVNKLV
SIAKDSINGDVRINGYFSNNGTGNYKLVMSDEFNGEDGTQPDSKIWSYCSREHPTWKRFV
AQSAEGKAKTAFIQDGKLVTRCIPNTLKGEGNVEMVSGAIESSGKVKIKYGYIESRLRTT
AHTGNFPAFWMMPQDNSAGWPNAGEIDIFEQIDNEDRSYHTIHSHWANGAADGGLGNSGK
PQKSASSATTTGDYHVYAINWSEKVIKWFVNGKQVFSYAKSTNQADLDKLQWPFDKPFYL
ILNQSVGNGSWAKPCDPTFTYETVFDYVRVYQTDGQSISVPTGIAEAETSSLDFYVRQGK
VLLVAPDEQHVSIYDLQGRAVFSEMVQGNRTVYLPKGVYVLNGKKIMVP

Enzyme Prediction     

No EC number prediction in MGYG000003489_02080.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	810	1051	8.9e-55	0.991304347826087

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd08023	GH16_laminarinase_like	2.14e-60	810	1051	2	235	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
cd02182	GH16_Strep_laminarinase_like	1.69e-26	805	1052	2	259	Streptomyces laminarinase-like, member of glycosyl hydrolase family 16. Proteins similar to Streptomyces sioyaensis beta-1,3-glucanase (laminarinase) present in Actinomycetales as well as Peziomycotina. Laminarinases belong to glycosyl hydrolase family 16 and hydrolyze the glycosidic bond of the 1,3-beta-linked glucan, a major component of fungal and plant cell walls and the structural and storage polysaccharides (laminarin) of marine macro-algae. Members of the GH16 family have a conserved jelly roll fold with an active site channel.
cd00413	Glyco_hydrolase_16	2.97e-24	811	1051	1	210	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
cd02178	GH16_beta_agarase	3.25e-23	799	1052	13	258	Beta-agarase, member of glycosyl hydrolase family 16. Beta-agarase is a glycosyl hydrolase family 16 (GH16) member that hydrolyzes the internal beta-1,4-linkage of agarose, a hydrophilic polysaccharide found in the cell wall of Rhodophyceaea, marine red algae. Agarose is a linear chain of galactose units linked by alternating L-alpha-1,3- and D-beta-1,4-linkages that are additionally modified by a 3,6-anhydro-bridge. Agarose forms thermo-reversible gels that are widely used in the food industry or as a laboratory medium. While beta-agarases are also found in two other families derived from the sequence-based classification of glycosyl hydrolases (GH50, and GH86) the GH16 members are most abundant. This domain adopts a curved beta-sandwich conformation, with a tunnel-shaped active site cavity, referred to as a jellyroll fold.
cd08024	GH16_CCF	4.33e-21	877	1027	101	275	Coelomic cytolytic factor, member of glycosyl hydrolase family 16. Subgroup of glucanases of unknown function that are related to beta-GRP (beta-1,3-glucan recognition protein), but contain active site residues. Beta-GRPs are one group of pattern recognition receptors (PRRs), also referred to as biosensor proteins, that complexes with pathogen-associated beta-1,3-glucans and then transduces signals necessary for activation of an appropriate innate immune response. Beta-GRPs are present in insects and lack all catalytic residues. This subgroup contains related proteins that still contain the active site and are widely distributed in eukaryotes. Their structures adopt a jelly roll fold with a deep active site channel harboring the catalytic residues, like those of other glycosyl hydrolase family 16 members.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QFQ12302.1	2.10e-134	1	751	5	734
QFQ13169.1	9.70e-88	510	1052	20	541
QUB65408.1	1.32e-75	805	1127	48	333
QUB68183.1	2.50e-75	805	1127	48	333
QUB63276.1	4.74e-75	805	1127	48	333

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6XOF_A	2.12e-30	805	1052	24	267	ChainA, GH16 family protein [uncultured bacterium]
3ATG_A	5.23e-30	807	1057	5	244	endo-1,3-beta-glucanasefrom Cellulosimicrobium cellulans [Cellulosimicrobium cellulans]
3ILN_A	6.27e-30	805	1052	5	248	ChainA, Laminarinase [Rhodothermus marinus],3ILN_B Chain B, Laminarinase [Rhodothermus marinus]
6XQF_A	9.75e-30	805	1052	24	267	ChainA, GH16 family protein [uncultured bacterium],6XQG_A Chain A, GH16 family protein [uncultured bacterium],6XQH_A Chain A, GH16 family protein [uncultured bacterium],6XQL_A Chain A, GH16 family protein [uncultured bacterium],6XQM_A Chain A, GH16 family protein [uncultured bacterium]
4DFS_A	1.71e-29	805	1052	18	263	Structureof the catalytic domain of an endo-1,3-beta-glucanase (laminarinase) from Thermotoga petrophila RKU-1 [Thermotoga petrophila RKU-1],4DFS_B Structure of the catalytic domain of an endo-1,3-beta-glucanase (laminarinase) from Thermotoga petrophila RKU-1 [Thermotoga petrophila RKU-1]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9ZG90	1.31e-38	802	1052	53	288	Keratan-sulfate endo-1,4-beta-galactosidase OS=Sphingobacterium multivorum OX=28454 PE=1 SV=1
P45798	4.33e-29	805	1052	40	283	Beta-glucanase OS=Rhodothermus marinus OX=29549 GN=bglA PE=1 SV=1
P23903	6.77e-24	807	1052	425	680	Glucan endo-1,3-beta-glucosidase A1 OS=Niallia circulans OX=1397 GN=glcA PE=1 SV=1
Q27082	4.80e-17	805	1075	25	269	Clotting factor G alpha subunit OS=Tachypleus tridentatus OX=6853 PE=1 SV=1
C1IE32	2.22e-14	803	1038	18	249	Glucan endo-1,3-beta-glucosidase OS=Cryptopygus antarcticus OX=187623 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000331	0.998966	0.000196	0.000162	0.000152	0.000143

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003489_02080.