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CAZyme Information: MGYG000003491_00380
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Basic Information |
Genomic context |
Full Sequence |
Enzyme annotations |
CAZy signature domains |
CDD domains |
CAZyme hits |
PDB hits |
Swiss-Prot hits |
SignalP and Lipop annotations |
TMHMM annotations
Basic Information help
| Species | UBA2804 sp900768635 | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Lineage | Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Succinivibrionaceae; UBA2804; UBA2804 sp900768635 | |||||||||||
| CAZyme ID | MGYG000003491_00380 | |||||||||||
| CAZy Family | GH13 | |||||||||||
| CAZyme Description | Pullulanase | |||||||||||
| CAZyme Property |
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| Genome Property |
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| Gene Location | Start: 115; End: 1584 Strand: + | |||||||||||
CAZyme Signature Domains help
| Family | Start | End | Evalue | family coverage |
|---|---|---|---|---|
| GH13 | 4 | 313 | 1.5e-96 | 0.7313019390581718 |
CDD Domains download full data without filtering help
| Cdd ID | Domain | E-Value | qStart | qEnd | sStart | sEnd | Domain Description |
|---|---|---|---|---|---|---|---|
| PLN02877 | PLN02877 | 3.57e-98 | 6 | 445 | 479 | 916 | alpha-amylase/limit dextrinase |
| cd11341 | AmyAc_Pullulanase_LD-like | 2.44e-94 | 6 | 354 | 120 | 401 | Alpha amylase catalytic domain found in Pullulanase (also called dextrinase; alpha-dextrin endo-1,6-alpha glucosidase), limit dextrinase, and related proteins. Pullulanase is an enzyme with action similar to that of isoamylase; it cleaves 1,6-alpha-glucosidic linkages in pullulan, amylopectin, and glycogen, and in alpha-and beta-amylase limit-dextrins of amylopectin and glycogen. Pullulanases are very similar to limit dextrinases, although they differ in their action on glycogen and the rate of hydrolysis of limit dextrins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. |
| TIGR02104 | pulA_typeI | 1.41e-55 | 4 | 455 | 240 | 591 | pullulanase, type I. Pullulan is an unusual, industrially important polysaccharide in which short alpha-1,4 chains (maltotriose) are connected in alpha-1,6 linkages. Enzymes that cleave alpha-1,6 linkages in pullulan and release maltotriose are called pullulanases although pullulan itself may not be the natural substrate. This family consists of pullulanases related to the subfamilies described in TIGR02102 and TIGR02103 but having a different domain architecture with shorter sequences. Members are called type I pullulanases. |
| COG1523 | PulA | 2.45e-34 | 6 | 344 | 278 | 575 | Pullulanase/glycogen debranching enzyme [Carbohydrate transport and metabolism]. |
| TIGR02102 | pullulan_Gpos | 9.04e-18 | 6 | 468 | 568 | 972 | pullulanase, extracellular, Gram-positive. Pullulan is an unusual, industrially important polysaccharide in which short alpha-1,4 chains (maltotriose) are connected in alpha-1,6 linkages. Enzymes that cleave alpha-1,6 linkages in pullulan and release maltotriose are called pullulanases although pullulan itself may not be the natural substrate. In contrast, a glycogen debranching enzyme such GlgX, homologous to this family, can release glucose at alpha,1-6 linkages from glycogen first subjected to limit degradation by phosphorylase. Characterized members of this family include a surface-located pullulanase from Streptococcus pneumoniae () and an extracellular bifunctional amylase/pullulanase with C-terminal pullulanase activity (. |
CAZyme Hits help
| Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End |
|---|---|---|---|---|---|
| VEC96347.1 | 1.19e-118 | 1 | 487 | 231 | 707 |
| AIE01821.1 | 1.58e-116 | 1 | 487 | 536 | 1012 |
| ASR24694.1 | 1.94e-116 | 1 | 487 | 601 | 1077 |
| ASR32196.1 | 1.94e-116 | 1 | 487 | 601 | 1077 |
| ALD57539.1 | 1.94e-116 | 1 | 487 | 601 | 1077 |
PDB Hits download full data without filtering help
| Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
|---|---|---|---|---|---|---|
| 6J33_A | 2.67e-116 | 1 | 487 | 561 | 1037 | ChainA, pullulanase [Klebsiella pneumoniae],6J33_B Chain B, pullulanase [Klebsiella pneumoniae],6J34_A Chain A, Pullulanase [Klebsiella pneumoniae] |
| 6J35_A | 7.64e-115 | 1 | 487 | 561 | 1037 | ChainA, Pullulanase [Klebsiella pneumoniae],6J35_B Chain B, Pullulanase [Klebsiella pneumoniae],6J4H_A Chain A, Pullulanase [Klebsiella pneumoniae] |
| 2FGZ_A | 1.31e-114 | 1 | 487 | 591 | 1067 | ChainA, Alpha-dextrin endo-1,6-alpha-glucosidase [Klebsiella pneumoniae],2FH6_A Chain A, Alpha-dextrin endo-1,6-alpha-glucosidase [Klebsiella pneumoniae],2FH8_A Chain A, Alpha-dextrin endo-1,6-alpha-glucosidase [Klebsiella pneumoniae],2FHB_A Chain A, Alpha-dextrin endo-1,6-alpha-glucosidase [Klebsiella pneumoniae],2FHC_A Chain A, Alpha-dextrin endo-1,6-alpha-glucosidase [Klebsiella pneumoniae],2FHF_A Chain A, Alpha-dextrin endo-1,6-alpha-glucosidase [Klebsiella pneumoniae] |
| 5YN2_A | 1.81e-114 | 1 | 487 | 610 | 1086 | ChainA, PulA protein [Klebsiella pneumoniae],5YN7_A Chain A, PulA protein [Klebsiella pneumoniae],5YNA_A Chain A, PulA protein [Klebsiella pneumoniae],5YNC_A Chain A, PulA protein [Klebsiella pneumoniae],5YND_A Chain A, DUF3372 domain-containing protein [Klebsiella pneumoniae],5YNE_A Chain A, PulA protein [Klebsiella pneumoniae],5YNH_A Chain A, PulA protein [Klebsiella pneumoniae] |
| 2YOC_A | 1.46e-108 | 1 | 487 | 579 | 1055 | ChainA, Pullulanase [Klebsiella oxytoca],2YOC_B Chain B, Pullulanase [Klebsiella oxytoca] |
Swiss-Prot Hits download full data without filtering help
| Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
|---|---|---|---|---|---|---|
| P07206 | 1.05e-115 | 1 | 487 | 598 | 1074 | Pullulanase OS=Klebsiella pneumoniae OX=573 GN=pulA PE=1 SV=2 |
| P07811 | 1.34e-111 | 1 | 435 | 608 | 1015 | Pullulanase OS=Klebsiella aerogenes OX=548 GN=pulA PE=1 SV=1 |
| Q8GTR4 | 1.39e-71 | 6 | 445 | 472 | 909 | Pullulanase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PU1 PE=1 SV=2 |
| O33840 | 1.28e-26 | 5 | 414 | 456 | 769 | Pullulanase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=pulA PE=1 SV=2 |
| C0SPA0 | 2.76e-24 | 5 | 339 | 328 | 597 | Pullulanase OS=Bacillus subtilis (strain 168) OX=224308 GN=amyX PE=1 SV=1 |
SignalP and Lipop Annotations help
This protein is predicted as OTHER
| Other | SP_Sec_SPI | LIPO_Sec_SPII | TAT_Tat_SPI | TATLIP_Sec_SPII | PILIN_Sec_SPIII |
|---|---|---|---|---|---|
| 1.000053 | 0.000000 | 0.000000 | 0.000000 | 0.000000 | 0.000000 |