CAZyme Information: MGYG000003525_00024

Basic Information

• Species: RC9 sp004556005
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; UBA932; RC9; RC9 sp004556005
• CAZyme ID: MGYG000003525_00024
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase BoGH2A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
831	MGYG000003525_1|CGC1	92437.56	5.4381

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003525	2213098	MAG	Fiji	Oceania

• Gene Location: Start: 33088; End: 35583 Strand: +

Full Sequence      

MKFLIISLLLALSPVSDRSASHFGQEAPQPSQPSQISQPSQPSQPSQSLRTTSESAASTL
RPLRESVLLDEGWQFAFGDASSPEKDFGCGTEYFNYLTKAASIHNAGPYSPGFDASDWQI
VDLPHDWVTLLPYSPDASHSHGYKQIGYAYPQNSVGWYRKTFTLPQDGSDRIYWLQFDGI
FRNCSVFLNGFYLGGEASGYATQIYDITQYLRFGEDNLLCVRVDASLEEGWFYEGAGIYR
HVRLHSASPVSIAPFGQFVYALWPREDCSEAVLHVETQLRNRSLSAAEVSVRHTLYDPAG
EAVLSFGQNCSGRLNSFRELTLECSVPVESPQLWSPESPRLYSLKTEVIAGGRTIDSLRT
SLGLRRVEWDPDRGLVLNASPYKIKGVNMHQDHAGVGTAIPDGLQEWRLMQLKEFGCNAY
RASHNPMTPDMLDACDRLGMLVIEENRLMGVNREHIDLLERMIRRDRNHPSVILWSVGNE
EWGIEWNDAGTRIASAMCSYCHRFDPTRKVTVATSGGPAPLLGVDVAGYNYILQNPVDRY
REQMPSRIALGSEETSGCGTRGVYFDDPELGRMASLNRSPQGPDSTLNCIGRGWKFYDSR
PWLAGLFYWTGFDYRGEPNPLKYPATGSQFGILDHCGFPKDEAWYLKSWWTDEDVIHILP
HWNLAGHEGERLSVWVYSNCDEVQLTVNGKRLERKKMERGGYLSWDVTYRPGTVVAQGYR
NGRKSVREVISTTGPAASVRADCERVGDVIVASFSLVDAQGSVVPDACEPVRVSFGKGVE
LLGAGNGDSAYRDPDRPACDGGSFVLNSFNGLAQAIFRVRGDDTAISASLL

Enzyme Prediction     

No EC number prediction in MGYG000003525_00024.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	50	655	4.1e-111	0.6356382978723404

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	2.59e-56	111	511	35	430	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	3.18e-51	52	510	26	472	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK10150	PRK10150	2.68e-37	153	514	62	449	beta-D-glucuronidase; Provisional
PRK09525	lacZ	1.41e-35	53	510	39	485	beta-galactosidase.
pfam02836	Glyco_hydro_2_C	2.07e-27	372	627	5	278	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADV42267.1	0.0	61	821	20	797
QBJ17990.1	0.0	63	822	22	790
QMI79437.1	0.0	63	822	22	790
BBK85901.1	0.0	63	822	22	790
SIP56251.1	0.0	63	822	22	790

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4YPJ_A	7.67e-128	106	816	27	789	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
7CWD_A	2.73e-125	111	812	26	779	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
5T98_A	4.51e-112	107	801	43	786	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]
3GM8_A	5.39e-102	65	799	5	760	ChainA, Glycoside hydrolase family 2, candidate beta-glycosidase [Phocaeicola vulgatus ATCC 8482]
6QUB_B	2.62e-98	111	801	29	820	Truncatedbeta-galactosidase III from Bifidobacterium bifidum in complex with galactose [Bifidobacterium bifidum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	2.45e-119	111	823	64	850	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	5.57e-85	114	788	71	769	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	1.25e-57	116	731	77	717	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
T2KN75	1.16e-51	113	765	50	724	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1
P77989	7.10e-51	109	783	23	695	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.035242	0.949346	0.014515	0.000334	0.000265	0.000264

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003525_00024.