CAZyme Information: MGYG000003528_01140

Basic Information

• Species: CAAGGB01 sp900769285
• Lineage: Bacteria; Verrucomicrobiota; Lentisphaeria; UBA1407; UBA1407; CAAGGB01; CAAGGB01 sp900769285
• CAZyme ID: MGYG000003528_01140
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1385		153703.5	7.838

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003528	3662993	MAG	Fiji	Oceania

• Gene Location: Start: 1732; End: 5889 Strand: -

Full Sequence      

MRHLLLPLIAAVAPALVSVGAPLPPYAPAPTPRERVAVRPVASLELPALPGGVAVRPSSY
TERVSLNGTWRISKAERSGKPFAAEELRPEASRVDFDDASWEDIRVPFDLGRQVRGLRDD
RSRPYVRVWYRRTFRLTPEQVSGRRVTLHFERIGYEADVFVNGRRLPVRHSGDFTPFSAD
VTELVQAGENAVSLRVLSDMGPAFGLPTGARHVYGSQWSTGNIRMGLWGDVTLVSEPCVG
IGRLLVTPDIGRKSAEVDVSLSPLPAYAGRRLRLAAAITPATASLGAAVCATVATAAATP
DKLPTRLTLACGGDIRLWDVESPNLYYCTLFVLDEEGRVLNARTERFGFREFRTENGHFL
LNGRRIYLFGENFASMNVGGMGRTPEEEDTYITEYIATMRSYGYVMLRNAHQPILPKTLA
IADELGMMFFNEWGFCFTNKLDEPAFERNNLAEVRRFVEETYNYASVTMWSMGNEVSHSM
KPDVVRQLDRQAALVRELDRQRRPISTFSGAAGWTVYGRNPLLTDVHDLHNYTALSDNWT
GLHGMFDYIIKGIGEIYGEKLPLSRPLVDWENVGFSWGWRHDATFRQDNIEDYLKYANGS
TTWGQPNGIGFSGCLPLHLALSPSHNHHLPQNIYGRRIFELFRLYPGWSGFAPWFSNAKL
DVATLWNQPVLPTLHNRRFFPPRNLFAGEPHATLLEISNCGDGDFADVTLQVTLASQSDG
SVTPVAIQASPVAVRPHRNSTAVEATLAIPSSVRPGYYQLRLDAMAGGVRIGQNYYDVFV
QARPAVTAPIRAVRPVFVLDTGVAANVRALTERLTALQVAHTVTASPDGLPADALLIVPP
ELEPQLLMRALAQRLESFVHGGGFLLVLEQFSPGTELPCNLVISAYALTFADPIRTSHPI
FRNLDWRNFDSWEDGGLRYVTLASYKPISVNALAARGIKLGQKAKDNIGMAALEGTYGRG
RLLASQFNAFRACQQDDASALTYLRNLLDYACAQEQPFHDIRPLAIRERDNYVVAERRAV
RIDLSRHTNRSFSDDVDGDRKGGWTDQGDNDFSNMPLGQQTLAGVPFTILDPAKNGGTSC
IVLAGTQRSYFPLAAKEIPLKGRFSRVFLLHTAAWGGAQNVGAYRFRYADGTTVTLPLVG
NQNIGDWWYSSAMPNARTGLILPHKVSGSYVCAYVTEWLNPHPLKELVSFDFLSPLYRER
EDIDFLPTATGVPVLIAATAERAHEQPFEIVPGRVTSWSGIATPPKTTSAVSTVIDDGTG
KGKLWQCVIPDTPAGDEPVAFFSHRRDNDQLADRYDYLTLRIRANSPARLRVTIPSMDWK
SNATATLVLIGDNQFHTYRFRFGQELKAPGSFALKNWRGELFFYFVNDGRPRKGVTFAVQ
SAVLE

Enzyme Prediction     

No EC number prediction in MGYG000003528_01140.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	60	525	8e-78	0.48936170212765956

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	7.78e-35	57	549	6	482	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	8.28e-26	66	475	44	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK10150	PRK10150	1.30e-21	62	506	11	446	beta-D-glucuronidase; Provisional
PRK09525	lacZ	7.83e-15	56	478	47	465	beta-galactosidase.
pfam02837	Glyco_hydro_2_N	7.68e-10	65	237	3	169	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AVM45809.1	0.0	5	1385	6	1369
AVM44624.1	0.0	15	1385	14	1372
AVM43171.1	0.0	45	1385	44	1374
AVM45701.1	0.0	15	1385	19	1377
AVM46610.1	2.66e-304	2	1364	3	1341

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7CWD_A	3.94e-21	63	502	3	431	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
6ED1_A	1.68e-20	59	502	22	447	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Phocaeicola dorei],6ED1_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Phocaeicola dorei],6ED1_C Chain C, Glycosyl hydrolase family 2, sugar binding domain protein [Phocaeicola dorei],6ED1_D Chain D, Glycosyl hydrolase family 2, sugar binding domain protein [Phocaeicola dorei]
5EUV_A	2.78e-19	142	615	65	487	ChainA, Beta-D-galactosidase [Paracoccus sp. 32d],5EUV_B Chain B, Beta-D-galactosidase [Paracoccus sp. 32d],5LDR_B Chain B, Beta-D-galactosidase [Paracoccus sp. 32d]
5LDR_A	2.78e-19	142	615	66	488	ChainA, Beta-D-galactosidase [Paracoccus sp. 32d]
4JKM_A	4.29e-18	64	571	16	496	CrystalStructure of Clostridium perfringens beta-glucuronidase [Clostridium perfringens str. 13],4JKM_B Crystal Structure of Clostridium perfringens beta-glucuronidase [Clostridium perfringens str. 13],6CXS_A Crystal Structure of Clostridium perfringens beta-glucuronidase bound with a novel, potent inhibitor 4-(8-(piperazin-1-yl)-1,2,3,4-tetrahydro-[1,2,3]triazino[4',5':4,5]thieno[2,3-c]isoquinolin-5-yl)morpholine [Clostridium perfringens str. 13],6CXS_B Crystal Structure of Clostridium perfringens beta-glucuronidase bound with a novel, potent inhibitor 4-(8-(piperazin-1-yl)-1,2,3,4-tetrahydro-[1,2,3]triazino[4',5':4,5]thieno[2,3-c]isoquinolin-5-yl)morpholine [Clostridium perfringens str. 13]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KPJ7	3.37e-24	97	476	75	431	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
Q7MG04	1.59e-18	56	478	40	460	Beta-galactosidase OS=Vibrio vulnificus (strain YJ016) OX=196600 GN=lacZ PE=3 SV=1
Q8D4H3	6.21e-18	56	478	40	461	Beta-galactosidase OS=Vibrio vulnificus (strain CMCP6) OX=216895 GN=lacZ PE=3 SV=2
P81650	6.24e-18	56	597	40	588	Beta-galactosidase OS=Pseudoalteromonas haloplanktis OX=228 GN=lacZ PE=1 SV=2
Q59140	9.32e-17	58	475	24	434	Beta-galactosidase OS=Arthrobacter sp. (strain B7) OX=86041 GN=lacZ PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.100245	0.896238	0.002383	0.000409	0.000320	0.000366

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003528_01140.