CAZyme Information: MGYG000003546_02156

Basic Information

• Species: UBA1181 sp900769555
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Paludibacteraceae; UBA1181; UBA1181 sp900769555
• CAZyme ID: MGYG000003546_02156
• CAZy Family: GH23
• CAZyme Description: DNA mismatch repair protein MutS

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
3048		348461.36	4.5065

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003546	3032937	MAG	Fiji	Oceania

• Gene Location: Start: 850; End: 9996 Strand: +

Full Sequence      

MFNDEVGKVTNNLDNWTSFLRTASNNFKYNFVEQVLIYAQRPDATACAEIGFWNDKMHRW
VNRGATGIALFDYSGSYQKLRYVFDVSDTNSFYGYEVPRWEVKDRYHDEVAEALTNAFGD
TAEGGLESVIDDTARNMVEDNILDYLSTVNDSKYGSFLEELDDDNISVILKNALSASIGY
MMMTRCGINADEYYTREDFEDIFNFNTPSSVIAMGMAVSDIAEMGLREIESTVKNIEKNE
KKQNRTFVKAKETEYDEGVKTKNETERSNENVTDDLQKSGRLSDSEPDLTNRGERYSWEV
RYDEKEIPAGTQESLFRTTDSEGRADENNQADGNRSGTDDDSARRTVAEVTGSDGRTERA
EPNQVGSDDEQHQNDSRGNGSEGTDLQLSGHDFDARWNGIEYFHQDKQKNELIKSFLTPY
REEIAAFYESHSDRKERGDFVMSFFNSIPFEMTLSNGAKAGFEAYSDAIRLWRNDGTELR
EAWEKWFQIEDSVFGMILIEEWTEPQALLLPSEDMQLEFIGTKVKDAILPLPQGAIDYVL
GAGSHFRESKMRIYRQFTESLSKEDNIKFLKNEYGTGGSSDAIPGTGYWENHDAKGIEIS
DHYSVPERRTLLKWNYVEKRISELIKFDRYLSPKEKETYPQWLENKLIEEAQWKKTSEIR
QIIREAPEEEQAPKEYRYEFNLGETVYIGADEYTILSLEDPVILNDSQYPLFNKEFSKAD
FEKKVKENPANNHLRIEVEVERTIIDDEPEIEVDESNKPDFLVQYEQIKKENPDGVLFMH
TGGFYVAFGEDAETVAKYTHNPAPRKNFYGNVFTTCCEVLDFNLSYCQKELYANNNINIV
FMEIDKGVVDRFPTPSVEITEQLDANYTDAFFINDGSNNVEWVYFNPDGNDGKGQFVNNL
ITYEDILKAAELHTDEKEFFDYLGSVAKQTLSDYGTKEYKDDFEYFHSAVDLTDCTKETM
QAIIEEAKSMEQPVITFVQPETKETEVLYKVLSALKIDDIELTYEGDILVARDSDDYTWS
GNEIYKFLIDDCFVFQDDGTVLGIDKELLEEFKTLSKEHDVTFADNRYSPLYRGYLEVKN
DNPNNIVLYQVGDFFEAFNDDAKTVAEALELVLTSRNIGGDKRVPLAGFPKHRLEAFLDM
LTDRGFDVAVCSLENGERKTYMIVSQNKEDPVESKVVGRIDYFHTDGKVRESIEYTSEYQ
FIKDIKEETYYGTPMFIAVYADKEGVTIPLDFVRELDPPPHGLEIVPSPYLEKSLYEKLV
DFYEDIDFYGFRDTLEVGETKEDAVEKLANDLQYPESIDLAIKELENIIAEEGTDEKDIA
LAKTLIAALQDEKERISYIPDSELIGKEVIIDGDKYIIEKIDSVFGDVSMRDTTSIYPIN
RVEKIDFVREHLAPEKQEYTTKKVDEYPAVENGLPYDIVVETIKTEKPTLTPPTWEEKKE
KVTTLYPTIPDSEKHNFRITDDNLGVGGPKEKFRNNMAAINLLHELEFENRLATPEEQEI
LSKYVGFGGLADAFDESKANWADEYKELIVTLSPEEYAAARESTLTAFYTPPVVIRAMYE
ALGNMGFSEGNILEPSCGTGNFIGMLPDNMQKSKFFGVELDSLTGRIAQQLYQKSGITVQ
GYENASLPDSFFDVAIGNVPFGQFKVIDKKYDKNNWLIHDYFFGKTLDKVRPGGVIAFIT
SSGTMDKRNSNVRKYIAQRAELLGAIRLPNNTFKANAGTEVTSDIIFLQKRDTLVNEEPD
WVHLDRDENGNEYNKYFVDHPEMVLGEMVMESSQFGHSLTCKPFEERNLSDLLSEAVQNI
HAEITEFDISEIGEVEDNSVPADPNVKNFSFTVVEDKLYYRQNSRMNPVDVSATAESRIK
GMIKIRDCVRELIRLQTDDYPDSSIKSAQDRLNTLYDDFTKKYGLLNARANRLAFADDSS
YCLLCSLEIVGENGELKRKADMFTKRTIKPHVKVTSVSTASEALAVSIGEKARVDMDFMC
ELTGKTEQELFEELKGVIFLNPEYTNENGNDERYLPADEYLSGNVRKKLELARRTAELYP
DDYNINVEMLEKVQPKDLTASEISVRLGATWLPPEDVERFVYEILQTPRYAQWNIKVKYT
ELTGEWTIANKSYDRTNVVANNTYGTSRINGYKIIEDTLNLKDVRIFDYVEDEHGNKKPV
LNKKETAIAQGKQEMIKQAFQDWIWKDPNRRERLVRIYNDLFNSMRAREYDGSHITFSGM
NPEISLRPHQINAVARIMYGGNTLLAHVVGAGKTFEIVAAAQESKRLGLCNKSLIVVPNH
LTEQWASEYLQLYPSANILVATKKDFETKNRKKFCARIATGDYDAIIIGHSQFEKIPISI
ERQREMLQRQIDEITLGVAQIKYDRGEKITVKQLEKTKKSLQTKLDKLNDQSRKDDVVTF
EELGVDRLFVDEAHNFKNLFLYTKMRNVGGIAQTEAQKSSDLYMKCRYLDEVTGNKGVIF
ATGTPVSNSMVELYTMQRYLQYDALEDKHLQHFDSWASTFGETVTAIELAPEGTGYRAKT
RFAKFYNLPELMMMFRQVADIQTADMLNLPVPKANFHNIVVKPTEHQKQIVESLSERAEK
VRNKMVDSSVDNMLLITNDGRKLALDQRLFNEMLPDDPDSKVAACVDNVYGIWERTTPQK
STQLIFCDLSTPHNDGKFNVYDDIKKKLIAKGVPESEIAFIHNADSEAKKKDLFGKVRAG
QVRVLIGSTAKMGAGTNVQQKLIAIHHTDCPWRPADLQQREGRIVRQGNENPEVEIYSYV
TEQTFDAYLYQLVENKQKFIGQIMTSKSPVRSAEDVDEQALSYAEIKALATGNPYIKEKM
DLDVAVSKLKLLKQNHLSQRYSLEDRIIKFYPQQIKQGEERIAGYNADIQRAKENTFLNA
DNFSPMIVEDVTYDEKKGAGSAILVACKAMTLPDPKQIGSYRGFNMELSFNSFEKEYVLT
LVGSLRHSVSLGADIYGNITRLDNVISSMPDKLNACEERLADTKVQLENAKAEVERPFPQ
EEELAQKMERLAELNALLDMDHHDNEIVDGEVEQEERDKTVLDKSVER

Enzyme Prediction     

No EC number prediction in MGYG000003546_02156.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG4646	COG4646	5.23e-79	1754	2280	2	540	Adenine-specific DNA methylase, N12 class [Replication, recombination and repair].
COG4646	COG4646	2.23e-26	2421	2609	439	634	Adenine-specific DNA methylase, N12 class [Replication, recombination and repair].
pfam01624	MutS_I	5.58e-25	1070	1151	2	83	MutS domain I. This domain is found in proteins of the MutS family (DNA mismatch repair proteins) and is found associated with pfam00488, pfam05188, pfam05192 and pfam05190. The MutS family of proteins is named after the Salmonella typhimurium MutS protein involved in mismatch repair; other members of the family included the eukaryotic MSH 1,2,3, 4,5 and 6 proteins. These have various roles in DNA repair and recombination. Human MSH has been implicated in non-polyposis colorectal carcinoma (HNPCC) and is a mismatch binding protein. The aligned region corresponds with globular domain I, which is involved in DNA binding, in Thermus aquaticus MutS as characterized in.
COG0249	MutS	2.32e-18	1070	1177	8	96	DNA mismatch repair ATPase MutS [Replication, recombination and repair].
cd18011	DEXDc_RapA	6.85e-15	2226	2488	1	187	DEXH-box helicase domain of RapA. In bacteria, RapA is an RNA polymerase (RNAP)-associated SWI2/SNF2 (switch/sucrose non-fermentable) protein that mediates RNAP recycling during transcription. The ATPase activity of RapA is stimulated by its interaction with RNAP and inhibited by its N-terminal domain. The conformational changes of RapA and its interaction with RNAP are essential for RNAP recycling. RapA is part of the DEAD-like helicase superfamily, a diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ASV45029.1	0.0	1457	3014	1548	3186
QIW86704.1	0.0	1457	3014	1616	3254
QIW86628.1	0.0	1457	3014	1616	3254
AXF51455.1	3.35e-315	1457	3014	1782	3423
AEY69616.1	6.16e-315	1457	3014	1689	3330

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3K0S_A	5.11e-13	1068	1177	9	110	ChainA, DNA mismatch repair protein mutS [Escherichia coli K-12],3K0S_B Chain B, DNA mismatch repair protein mutS [Escherichia coli K-12]
1NG9_A	5.12e-13	1068	1177	10	111	ChainA, DNA mismatch repair protein MutS [Escherichia coli],1NG9_B Chain B, DNA mismatch repair protein MutS [Escherichia coli]
1OH5_A	5.12e-13	1068	1177	10	111	THECRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH A C:A MISMATCH [Escherichia coli],1OH5_B THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH A C:A MISMATCH [Escherichia coli],1OH6_A THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH AN A:A MISMATCH [Escherichia coli],1OH6_B THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH AN A:A MISMATCH [Escherichia coli],1OH7_A THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH A G:G MISMATCH [Escherichia coli],1OH7_B THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH A G:G MISMATCH [Escherichia coli],1OH8_A THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH AN UNPAIRED THYMIDINE [Escherichia coli],1OH8_B THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH AN UNPAIRED THYMIDINE [Escherichia coli],1W7A_A ATP bound MutS [Escherichia coli],1W7A_B ATP bound MutS [Escherichia coli],2WTU_A Crystal structure of Escherichia coli MutS in complex with a 16 basepair oligo containing an A.A mismatch. [Escherichia coli],2WTU_B Crystal structure of Escherichia coli MutS in complex with a 16 basepair oligo containing an A.A mismatch. [Escherichia coli],3ZLJ_A CRYSTAL STRUCTURE OF FULL-LENGTH E.COLI DNA MISMATCH REPAIR PROTEIN MUTS D835R MUTANT IN COMPLEX WITH GT MISMATCHED DNA [Escherichia coli K-12],3ZLJ_B CRYSTAL STRUCTURE OF FULL-LENGTH E.COLI DNA MISMATCH REPAIR PROTEIN MUTS D835R MUTANT IN COMPLEX WITH GT MISMATCHED DNA [Escherichia coli K-12]
7OTO_A	5.15e-13	1068	1177	16	117	ChainA, DNA mismatch repair protein MutS [Escherichia coli K-12],7OTO_B Chain B, DNA mismatch repair protein MutS [Escherichia coli K-12],7OU0_A Chain A, DNA mismatch repair protein MutS [Escherichia coli K-12],7OU0_B Chain B, DNA mismatch repair protein MutS [Escherichia coli K-12],7OU4_A Chain A, DNA mismatch repair protein MutS [Escherichia coli],7OU4_B Chain B, DNA mismatch repair protein MutS [Escherichia coli]
7AI5_A	5.34e-13	1068	1177	10	111	ChainA, DNA mismatch repair protein MutS [Escherichia coli],7AI5_B Chain B, DNA mismatch repair protein MutS [Escherichia coli],7AI7_A Chain A, DNA mismatch repair protein MutS [Escherichia coli K-12],7AI7_B Chain B, DNA mismatch repair protein MutS [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q71TF8	9.06e-35	1476	2579	51	1224	Defense against restriction protein B OS=Escherichia phage P1 OX=2886926 GN=darB PE=3 SV=1
B0CF30	5.77e-17	1045	1151	8	116	DNA mismatch repair protein MutS OS=Acaryochloris marina (strain MBIC 11017) OX=329726 GN=mutS PE=3 SV=1
A1BHN5	1.69e-16	1068	1179	8	105	DNA mismatch repair protein MutS OS=Chlorobium phaeobacteroides (strain DSM 266) OX=290317 GN=mutS PE=3 SV=1
Q8KCC0	1.70e-16	1069	1151	13	95	DNA mismatch repair protein MutS OS=Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) OX=194439 GN=mutS PE=3 SV=1
B3QPY5	6.61e-16	1069	1151	13	95	DNA mismatch repair protein MutS OS=Chlorobaculum parvum (strain DSM 263 / NCIMB 8327) OX=517417 GN=mutS PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999891	0.000093	0.000031	0.000001	0.000000	0.000011

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003546_02156.