CAZyme Information: MGYG000003559_00996

Basic Information

• Species: HGM04593 sp900769765
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; HGM04593; HGM04593 sp900769765
• CAZyme ID: MGYG000003559_00996
• CAZy Family: GH85
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1163	MGYG000003559_86|CGC1	128004.77	4.9136

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003559	1756049	MAG	Fiji	Oceania

• Gene Location: Start: 17193; End: 20684 Strand: +

Full Sequence      

MKKIYFMLFALICMVIPAKAAVVTSLDQLSNDKLYTIRSARGFLMYSANVSTQIAFSTGK
SVGNVQFNPQDPNQQFKIEYKNGKYYLYSVGAGKYVGNQGKYANSALIQLKLESSGGATY
RWKLGLGNYYMNSQEPGSDWPAGIVVDDWTKTDDGNSYRIDEVVEGEGGDTGGDTTSSEV
YDFSGFGDEALMELFYNALQQGRNYPTMEEFAAAGIQASDIAFIRSHVRRANIMSRADRV
KPNTFEKRNLFMNMPMDYGKDGAVGHPEAKFNADVFSMWQYVNLFGTWNHSIFQAPGAWV
DAAHRNGTDIMSGIKFFDTTGGRGEGSGSYEDLVSRKDANGNFKYVKPLINILMFFGSDG
INYNWEATGFDKTDVVNFHKQLYKYAEEIGFDSYHSAIYTSVSSLSNGSSNINALYGTKE
TGKTHDLMLNYQANDIAGGLASTYNAVVNAYGSAEGLYAGVWTASMDRNWTNLEGNNVGI
CLWGEHDQSRFYSFNAGDGVFNIQGNYQRLIERGFSGGNRNPANTPALSNTGNNWVADNG
KLPLQTFAGMAHWIPERSTIQGNLPFGTHFTLGNGDRYYYKGKMSHKSEWYNMSAQDRVP
TYRWLRYESDTYIVTEDIDVNYTHLDAYTGGSCIELTGKATSRGTDIILYKTSLRAADGA
YAKLAVKTLKDVKASNLYLILKKQGSNTWLEYPYGDVAGNTWEEKKFDLGGIASGDVIER
IGLRVKGSNNDYQLYVGKLEINDNSVVTPAEVKDLIAEVKMETKESMSLKLHWDVNAQAV
SRAAWGLVFNDEANIDHFEVLYKVGEDGKVALVGTTSQWATFVGNIEFESPEDVPFIGVR
SVSTDLKTYSPIVWVKVTRGDQAALPEKAEEDTYGISQMDPASNGADAARAQRYVELVKT
TGATQNLNYTASAPVADGTQYTHADPAQHLLKITQGQSVTMTIKGPQMSDGLQYCYGGGW
LDLNGSGNFDHPLPVERTASEIAKGTTETDPEGERIFFIGKHEGATPELVTGYTFTFKVP
EDATPGDSRLRMVFSDAWFAGAFNPTGLTNKGFTIDFSVEILGDNPSRAAVDTRDVGEAE
EPVLLEGGTETNIKETVSEVSVAEGAEGAIEFANVEKAWVYTVDGKFVKFANNKPAAIEV
KPGIYLVKMQNGNVIRNAKVMVK

Enzyme Prediction     

No EC number prediction in MGYG000003559_00996.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH85	266	579	5.1e-41	0.9619047619047619

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG4724	COG4724	2.64e-33	263	744	88	552	Endo-beta-N-acetylglucosaminidase D [Carbohydrate transport and metabolism].
cd06547	GH85_ENGase	6.85e-29	266	610	17	339	Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the protein and N-acetylglucosamine 2 forms the reducing end of the released glycan. ENGase is a key enzyme in the processing of free oligosaccharides in the cytosol of eukaryotes. Oligosaccharides formed in the lumen of the endoplasmic reticulum are transported into the cytosol where they are catabolized by cytosolic ENGases and other enzymes, possibly to maximize the reutilization of the component sugars. ENGases have an eight-stranded alpha/beta barrel topology and are classified as a family 85 glycosyl hydrolase (GH85) domain. The GH85 ENGases are sequence-similar to the family 18 glycosyl hydrolases, also known as GH18 chitinases. An ENGase-like protein is also found in bacteria and is included in this alignment model.
pfam03644	Glyco_hydro_85	6.00e-10	265	362	1	93	Glycosyl hydrolase family 85. Family of endo-beta-N-acetylglucosaminidases. These enzymes work on a broad spectrum of substrates.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCI64641.1	0.0	175	1159	32	1003
AGB28382.1	0.0	175	1163	201	1190
QUT74553.1	0.0	175	1159	28	1016
QUB47476.1	0.0	175	1163	32	1006
EFC71166.1	1.48e-312	175	1163	31	1006

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3FHQ_A	1.58e-28	220	843	26	603	ChainA, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_B Chain B, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_D Chain D, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_F Chain F, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae]
2VTF_A	5.08e-28	220	843	31	608	X-raycrystal structure of the Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae E173Q mutant reveals a TIM barrel catalytic domain and two ancillary domains [Glutamicibacter protophormiae],2VTF_B X-ray crystal structure of the Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae E173Q mutant reveals a TIM barrel catalytic domain and two ancillary domains [Glutamicibacter protophormiae]
3FHA_A	6.54e-28	220	843	26	603	ChainA, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_B Chain B, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_C Chain C, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_D Chain D, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000281	0.998941	0.000285	0.000159	0.000156	0.000155

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003559_00996.