CAZyme Information: MGYG000003564_01511

Basic Information

• Species: UBA1232 sp004561775
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; UBA932; UBA1232; UBA1232 sp004561775
• CAZyme ID: MGYG000003564_01511
• CAZy Family: GH9
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
876	MGYG000003564_312|CGC1	99188.61	5.6187

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003564	1730489	MAG	Fiji	Oceania

• Gene Location: Start: 1867; End: 4497 Strand: +

Full Sequence      

MINLITYITLALSMLAQPNNTLAAKSSDSLLQDNRKALSTPEIIAKEQKCQDQSGAKSWI
RVNQLGYLPNDVKVAVYISAEDESASTATADTFRVFRALDGKVLFSGKAQQKDASKWALK
SAFRLDFSPLREEEYCYIEFAGARSPLFRIGSNVYEGLSDYLLTYMRQQRCGYNPFNNAK
CHTHDGYIVDHPTRSGQKIDVTGGWHDATDYLQYQTTTATTIYQMLFAYMEQPDKSIFKD
EYNAAGNRGSNGIPDILDEARWGLEWLLKMNPQNKVMFSQIADDRDHAGFRLPYKDSVDY
GWGRGEGRPVYFVTGKPQRVYKGLNRTTGVSSVAGKFSSTFALGAEVFKEIEPRLSKKML
VKASQAYAYAEELPGNTQTISIVSPYFYEEDTYTDDIELAASTLYHYTKDPIWGKRADYW
GELEPVTPWMELGRGRHYQYYPFINLGHYYLASSEDKAIANKYREYMRMGLESIRRRAAE
DPFMNGIPYIWCSNNLTSAAITQARLYNKVSGDSTYMEMEAALRDWLFGCNPWGTSMITG
IPYYGDYPVAPHSSYYVINRDLTYGGLIDGPVYRSIFEERAGNSLTKEDRYARFNNGIAV
YHDDLGDYATNEPTMDGTAGLTYYFAAKECEGLLYREANKAPFEGEEDNYGVIRRINPSE
KSIYLLFTADSNFVGAESILKTLEKEKVKGSFFLTGNCLRMPENMAAVTAIVEAGHYVGG
HSDKHLLYAPWEGRDSSYYSPEEIIADINANFAELEKFGIERDSARFFIPPYEHYNRLSV
EAMRRAGLIPVNYTAGTATPADYTTPSMSNYIGAQELIARLYAFEAEKGLNGAMILIHPG
VSEERKDRLYDKLGEIIKYLKKLGYEFKSLNQICTK

Enzyme Prediction     

No EC number prediction in MGYG000003564_01511.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH9	155	626	1.5e-73	0.9952153110047847

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam00759	Glyco_hydro_9	1.87e-63	163	623	7	372	Glycosyl hydrolase family 9.
cd10917	CE4_NodB_like_6s_7s	4.42e-26	661	861	1	171	Catalytic NodB homology domain of rhizobial NodB-like proteins. This family belongs to the large and functionally diverse carbohydrate esterase 4 (CE4) superfamily, whose members show strong sequence similarity with some variability due to their distinct carbohydrate substrates. It includes many rhizobial NodB chitooligosaccharide N-deacetylase (EC 3.5.1.-)-like proteins, mainly from bacteria and eukaryotes, such as chitin deacetylases (EC 3.5.1.41), bacterial peptidoglycan N-acetylglucosamine deacetylases (EC 3.5.1.-), and acetylxylan esterases (EC 3.1.1.72), which catalyze the N- or O-deacetylation of substrates such as acetylated chitin, peptidoglycan, and acetylated xylan. All members of this family contain a catalytic NodB homology domain with the same overall topology and a deformed (beta/alpha)8 barrel fold with 6- or 7 strands. Their catalytic activity is dependent on the presence of a divalent cation, preferably cobalt or zinc, and they employ a conserved His-His-Asp zinc-binding triad closely associated with the conserved catalytic base (aspartic acid) and acid (histidine) to carry out acid/base catalysis. Several family members show diversity both in metal ion specificities and in the residues that coordinate the metal.
cd10950	CE4_BsYlxY_like	9.77e-22	657	873	2	187	Putative catalytic NodB homology domain of uncharacterized protein YlxY from Bacillus subtilis and its bacterial homologs. The Bacillus subtilis genome contains six polysaccharide deacetylase gene homologs: pdaA, pdaB (previously known as ybaN), yheN, yjeA, yxkH and ylxY. This family is represented by Bacillus subtilis putative polysaccharide deacetylase BsYlxY, encoded by the ylxY gene, which is a member of the carbohydrate esterase 4 (CE4) superfamily. Although its biological function still remains unknown, BsYlxY shows high sequence homology to the catalytic domain of Bacillus subtilis pdaB gene encoding a putative polysaccharide deacetylase (BsPdaB), which is essential for the maintenance of spores after the late stage of sporulation and is highly conserved in spore-forming bacteria. However, disruption of the ylxY gene in B. subtilis did not cause any sporulation defect. Moreover, the Asp residue in the classical His-His-Asp zinc-binding motif of CE4 esterases is mutated to a Val residue in this family. Other catalytically relevant residues of CE4 esterases are also not conserved, which suggest that members of this family may be inactive.
cd02850	E_set_Cellulase_N	5.11e-21	59	150	1	86	N-terminal Early set domain associated with the catalytic domain of cellulase. E or "early" set domains are associated with the catalytic domain of cellulases at the N-terminal end. Cellulases are O-glycosyl hydrolases (GHs) that hydrolyze beta 1-4 glucosidic bonds in cellulose. They are usually categorized into either exoglucanases, which sequentially release terminal sugar units from the cellulose chain, or endoglucanases, which also attack the chain internally. The N-terminal domain of cellulase may be related to the immunoglobulin and/or fibronectin type III superfamilies. These domains are associated with different types of catalytic domains at either the N-terminal or C-terminal end and may be involved in homodimeric/tetrameric/dodecameric interactions. Members of this family include members of the alpha amylase family, sialidase, galactose oxidase, cellulase, cellulose, hyaluronate lyase, chitobiase, and chitinase, among others.
pfam02927	CelD_N	1.24e-19	58	145	1	83	Cellulase N-terminal ig-like domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AGY53377.1	0.0	56	870	22	831
QIK60909.1	0.0	57	867	23	823
QIK55492.1	0.0	56	867	22	823
BBE19991.1	3.62e-306	65	865	1	787
AHW60805.1	6.35e-303	60	870	24	815

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3X17_A	1.33e-33	60	626	18	554	Crystalstructure of metagenome-derived glycoside hydrolase family 9 endoglucanase [uncultured bacterium],3X17_B Crystal structure of metagenome-derived glycoside hydrolase family 9 endoglucanase [uncultured bacterium]
4CJ0_A	2.12e-26	58	552	28	492	ChainA, ENDOGLUCANASE D [Acetivibrio thermocellus],4CJ1_A Chain A, ENDOGLUCANASE D [Acetivibrio thermocellus]
1CLC_A	2.27e-26	58	552	42	506	ChainA, ENDOGLUCANASE CELD; EC: 3.2.1.4 [Acetivibrio thermocellus]
5U2O_A	5.00e-26	68	623	2	534	Crystalstructure of Zn-binding triple mutant of GH family 9 endoglucanase J30 [Thermobacillus composti KWC4]
5U0H_A	8.07e-23	68	623	2	534	Crystalstructure of GH family 9 endoglucanase J30 [Thermobacillus composti KWC4]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P0C2S4	1.16e-25	58	552	28	492	Endoglucanase D (Fragment) OS=Acetivibrio thermocellus OX=1515 GN=celD PE=1 SV=1
A3DDN1	1.30e-25	58	552	52	516	Endoglucanase D OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=celD PE=1 SV=1
P23658	8.19e-20	60	623	4	540	Cellodextrinase OS=Butyrivibrio fibrisolvens OX=831 GN=ced1 PE=1 SV=1
A7LXT3	1.21e-18	60	621	32	571	Xyloglucan-specific endo-beta-1,4-glucanase BoGH9A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02649 PE=1 SV=1
P14090	9.32e-18	60	623	341	902	Endoglucanase C OS=Cellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547) OX=590998 GN=cenC PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000297	0.999052	0.000162	0.000167	0.000153	0.000143

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003564_01511.