CAZyme Information: MGYG000003572_00621

Basic Information

• Species: Prevotella sp900770025
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella; Prevotella sp900770025
• CAZyme ID: MGYG000003572_00621
• CAZy Family: GH137
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1346		154048.92	7.245

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003572	2050564	MAG	Fiji	Oceania

• Gene Location: Start: 25387; End: 29427 Strand: -

Full Sequence      

MTRKLILYIVLLLCSQAAMSQRKKTTRKQPAAIQQRVSQEEMRRIYEEVRTPYKFGMVMA
PQSNNRKFDCPTVFREGDKWYMTYVCYNGASGTDGRGYETWVAQSEDLLHWTTLGRILAF
GESGWDMNQRGGFPALIDYEWGGTYNIGKFKNRYWMTYIGGQGTGYEAVNAPLSIGLAST
AGDITTAHQWETYDKPIMTYNEKDAQWWEQMTQYKSTVYQVDKKLFGYPFMMYYNAGGKD
ETHPKGERIGVAFSNDMRTWKRYKGNPIFAHDTDGTITGDAQIVRMGNLYVMFYFSAFNP
TREYNAYNTFAVSRDMIHWYDWEGEDLIVPSKPYDEMFAHKSYVVKHNGVVYHFYCAVNN
AGQRGIAVATSIPMGKSEVNFPTPDPTGKRYTASLNDDWQIEHNKHIFTKDIPFNLDDYY
GALQKEHGNLHGKAVFRKVFRAPDLQDKEYFLRFEGVGTYAEVLLNGKHMGRYDIGRTTE
TINITKEVRRGTDNQLEVRVSHPQGITDMPWVCGGCSSEWGFSEGSQPFGIYRPVTLEVT
DKIRIEPFGVHIWNTPAADSIYIETEVKNYSDEMAAISLISKICESGGKQIYRLTDEITM
APGETRVIKQSTAIKEVKRWSPENPYLYKLNTIIKRNGKATDDLLTPFGVKTSSWPLTRK
DGDKRFFLNGKPVFINGICEYEHMFGQSHAFSPAQIEARIKEVKDAGFNAFRDAHQPHNL
LYQELIDREGILWWPQFSAHIWYDTPQFRESFKRHLIQWVKERRNSPSIILWGLQNESTL
PKEFAAECTEIIREMDPTCGTMRLITTCNGGEGTDWNVVQNWSGTYGGVLENYGKELSTN
EQLLNGEYGAWRTLGNHTGERYTEEKMCDILERKVILAEGAKDSVCGHFQWIMQSHDNPG
RRQPEEGLRRIDKVGPFNHKGILTAWEQPVDAYYMYKSHYVPASREPMVYIVPHLVSDSI
RVYSNCEQVTLSCGSWSSTLNKPQPSTCFVWKNIPLQGDVLTATAIHQGKQVVTDSLALP
SFRGWDESILHPEEGYHYLYNINCGGDEYTDVFGTNWQQDNTTWSSSWGSRFPEETSPYQ
ASQTSDCTLPVSPLFRTSRFGRHLLSYHFKAAPGTYRIELYFIEPWYGRGSSETADYEGI
RLFDVAVNDSTVIKDLDIWAQARYGKPYKRTLEIQHKGGEIKIDFPRINASQAIISAIAI
ASKHTTGNDITYPVVKNLWKNFDKDILVHTPDSLLPPKTNSAITAEGVLKKGTLTFTYNV
GVAKVYALRFRYYNPDKERTLRVKITDLNGVVYKEDDIIFVKTPEKKTKRRNTSITTGSQ
TNAGSYIVTLQGEGIEKMIFDKLTIE

Enzyme Prediction     

EC	3.2.1.31	3.2.1.185

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH137	34	381	2.8e-146	0.9941176470588236
GH2	389	850	5.7e-74	0.535904255319149
CBM57	1040	1198	1.2e-35	0.9863945578231292

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	2.49e-37	397	798	49	426	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam11721	Malectin	8.92e-23	1038	1189	1	144	Di-glucose binding within endoplasmic reticulum. Malectin is a membrane-anchored protein of the endoplasmic reticulum that recognizes and binds Glc2-N-glycan. It carries a signal peptide from residues 1-26, a C-terminal transmembrane helix from residues 255-274, and a highly conserved central part of approximately 190 residues followed by an acidic, glutamate-rich region. Carbohydrate-binding is mediated by the four aromatic residues, Y67, Y89, Y116, and F117 and the aspartate at D186. NMR-based ligand-screening studies has shown binding of the protein to maltose and related oligosaccharides, on the basis of which the protein has been designated "malectin", and its endogenous ligand is found to be Glc2-high-mannose N-glycan.
PRK10340	ebgA	2.65e-20	435	805	112	472	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK10150	PRK10150	3.08e-19	432	799	65	443	beta-D-glucuronidase; Provisional
pfam02836	Glyco_hydro_2_C	1.25e-14	663	864	5	226	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
SCV09658.1	0.0	34	1346	20	1399
QRQ56950.1	0.0	34	1346	20	1399
ALJ45323.1	0.0	34	1346	20	1399
QUT23622.1	0.0	34	1346	20	1396
QRN01915.1	0.0	34	1346	20	1399

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MUI_A	2.96e-179	34	385	26	378	Glycosidehydrolase BT_0996 [Bacteroides thetaiotaomicron VPI-5482]
5MT2_A	1.31e-172	34	385	26	378	Glycosidehydrolase BT_0996 [Bacteroides thetaiotaomicron VPI-5482],5MUJ_A BT0996 RGII Chain B Complex [Bacteroides thetaiotaomicron VPI-5482]
3CMG_A	2.79e-47	390	1019	4	653	Crystalstructure of putative beta-galactosidase from Bacteroides fragilis [Bacteroides fragilis NCTC 9343]
5Z1A_A	3.31e-47	390	1019	23	672	Thecrystal structure of Bacteroides fragilis beta-glucuronidase in complex with uronic isofagomine [Bacteroides fragilis NCTC 9343]
6D50_A	1.83e-42	381	1185	24	868	Bacteroidesuniforms beta-glucuronidase 2 bound to D-glucaro-1,5-lactone [Bacteroides uniformis str. 3978 T3 ii],6D50_B Bacteroides uniforms beta-glucuronidase 2 bound to D-glucaro-1,5-lactone [Bacteroides uniformis str. 3978 T3 ii]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KM09	6.94e-33	389	1012	45	700	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
A7LXS9	8.15e-30	396	777	68	437	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
P26257	1.20e-22	446	975	69	587	Beta-galactosidase OS=Thermoanaerobacterium thermosulfurigenes OX=33950 GN=lacZ PE=1 SV=1
P77989	2.03e-21	374	971	1	574	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
P06864	5.04e-21	399	778	80	450	Evolved beta-galactosidase subunit alpha OS=Escherichia coli (strain K12) OX=83333 GN=ebgA PE=1 SV=4

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000257	0.999132	0.000146	0.000154	0.000136	0.000133

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003572_00621.