CAZyme Information: MGYG000003573_01675

Basic Information

• Species: RC9 sp900769905
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; UBA932; RC9; RC9 sp900769905
• CAZyme ID: MGYG000003573_01675
• CAZy Family: GH115
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1014		114056.87	4.7814

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003573	2177308	MAG	Fiji	Oceania

• Gene Location: Start: 14500; End: 17544 Strand: +

Full Sequence      

MKPFKTLLITLLLCFQAASAKTAGDFDKPWTPGPYVIDNPAKDAFCISAKGRSASIVVDP
SDERGVARAARDLGTDIGRVSGVEAEVILSDKSRKGQIVAGTIGSSRLIDKYIAEGKLDV
GEILGTMEAFVIQTVEGRLVIAGSDRRGTIYGIYDISENIGVSPWYWWADVPAKHASSIY
VNAGRYLQPSPKVKYRGIFINDESPSFSGWSNLKFEDPDSRNGQNSLMYTHMFELLLRLK
ANYLWPAMWGKAFNEDDPRNPVLADEYGIIMGTSHHEPMMRAQQEYSDRQREVGIWDFVT
NRDNLLAFWREGIDRNKNYDNLITIGMRGDGDVPMGQGDDAHNAKVLEQVIADQRALISE
VYGKPASEVPQMWALFTEVQRYYDAGMTVPDDIMLMFCDNNWGYIRRTGPEKEKDRSGGM
GLYYHIDMNGGPWNDRWINTTTIPKLREQLGLAYSTGIDDLWVINVGDLKPKEIPIDFIM
RFAWNPDAIGADRTYDYTLSWAEENFGSELAEDVAWIVSRYPKYNLMRKPEVQNTGIFSY
ANYDEALRQTALWLELEDRAEALKARVPAELYDAFYQLVYYPAVASSGVARMYLGAGLNN
VHALQGNFRANSEAEEVLSLFERDAHLSESYNYEIASGKWKYMMSDIHIGYTQWFMHRFN
TLPTLYNLRPRRGAEMGVSISGSEKAWPEKEFELQVVDGTGLKREFVRKPQLDGKEPVLP
PFDAVGRQTYRIEVFNRAFGKLDYELSASHACVLLDDNGKGDVLVSIDWDKAPQGMSEHS
VQVRQKDASACVTVKLTANNVPAPAASRPYWGCLEGEYSVDATRPAANVAGSDARWTLLP
DLGRENGCMGITPVTSAFTGDFTDMPRLEYDILIPESGSKTICLGILPTQDVNPARGLRL
AVSIDDAEPVILDARKGFVDTYSEYTEENLARSQVLKPLPARKNYSLTSYDRLFRDEVFD
NLRWLDVDFDFSVSGIHTLKVWMVDPEIVLEKIIVGPDGRYSYSGPVPVEHLPR

Enzyme Prediction     

EC	3.2.1.131

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH115	43	721	2.3e-238	0.9096126255380201

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam15979	Glyco_hydro_115	0.0	190	532	2	334	Glycosyl hydrolase family 115. Glyco_hydro_115 is a family of glycoside hydrolases likely to have the activity of xylan a-1,2-glucuronidase, EC:3.2.1.131, or a-(4-O-methyl)-glucuronidase EC:3.2.1.-.
pfam17829	GH115_C	1.29e-33	819	1006	10	170	Gylcosyl hydrolase family 115 C-terminal domain. This domain is found at the C-terminus of glycosyl hydrolase family 115 proteins. This domain has a beta-sandwich fold.
pfam03648	Glyco_hydro_67N	1.44e-05	69	155	36	119	Glycosyl hydrolase family 67 N-terminus. Alpha-glucuronidases, components of an ensemble of enzymes central to the recycling of photosynthetic biomass, remove the alpha-1,2 linked 4-O-methyl glucuronic acid from xylans. This family represents the N-terminal region of alpha-glucuronidase. The N-terminal domain forms a two-layer sandwich, each layer being formed by a beta sheet of five strands. A further two helices form part of the interface with the central, catalytic, module (pfam07488).

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
EEC55419.1	0.0	42	1011	41	993
QRQ50360.1	0.0	42	1011	16	968
QDW23484.1	0.0	35	1014	35	990
QUT25621.1	0.0	1	1010	5	1008
QRQ59056.1	0.0	35	1010	5	979

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4ZMH_A	1.53e-222	54	1008	23	932	Crystalstructure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40],4ZMH_B Crystal structure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40]
4C90_A	4.08e-169	35	684	34	681	Evidencethat GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C90_B Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C91_A Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C91_B Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus]
7PUG_A	8.81e-163	45	668	17	650	ChainA, xylan alpha-1,2-glucuronidase [uncultured bacterium]
7PXQ_A	3.91e-160	45	668	16	649	ChainA, xylan alpha-1,2-glucuronidase [uncultured bacterium]
6NPS_A	5.38e-138	65	1014	31	968	Crystalstructure of GH115 enzyme AxyAgu115A from Amphibacillus xylanus [Amphibacillus xylanus NBRC 15112],6NPS_B Crystal structure of GH115 enzyme AxyAgu115A from Amphibacillus xylanus [Amphibacillus xylanus NBRC 15112]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000227	0.999102	0.000194	0.000155	0.000148	0.000135

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003573_01675.