CAZyme Information: MGYG000003627_01147

Basic Information

• Lineage: Bacteria; Verrucomicrobiota; Lentisphaeria; Victivallales; UBA1829; UBA1732
• CAZyme ID: MGYG000003627_01147
• CAZy Family: GH115
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
978		109267.82	6.0308

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003627	1872155	MAG	Fiji	Oceania

• Gene Location: Start: 2700; End: 5636 Strand: +

Full Sequence      

MINHRHCQLTGLLAGTLLATGSLFAADPFAGLVSRTHVDGGFRIAENGETASIIVSSNDY
PGVVRAAKDLGEDVKRVTGTASPVLTDGQPRPSSILVGTLGKNAAIDGMAESGAIDISAI
KGKWESYSIQTVGDILVIAGSDKRGTIYGIYEVSKRIGVSPWYWWADVPAVHADTLYVNS
GKYVQGPPKVKYRGIFINDEDPSFGGWCRTNFGGINSKMYAHMFELILRLKGNYLWPAMW
GKAFNEDDPLNPVVADEYGVVMGTSHHEPLMRNQQEWTKRSRQLGEWNYRENGDNMKKFW
LEGLKRNAKYDNLPTIGLRGDGDLPLDPNANFENNKELLHQVFHDQRELIREAYGRDPST
VPQIWVVFNEIVIFYNKGEVRPPEDVIMAICDSNFGYLLQFPAEKDRKHPESLGLYYHID
MNGGPWNDRWISTREIPQIWQQLYLAYEYGMRGLWIVNVGDLKPKEAEINFCLDYAWNPD
NIPFNQVDQWLVNWCAEQFPAEFAGETASLISRYIQLNHIRKPELQQTRIWSAVNYNEAD
QFAAEWQKIADRAEALKTKIPAAYADAYYQLVYYPVVAGAGTAQIYTEMAKADQFAKQGR
SSAEISVKKAQALFEKDQQLSDYYNSPQMASGKWKGMMADKHIGYTQWSMPQRNSLPRIG
KMPQLLEQSLLGVAAEGSEKAAPHQTESLALPVFDSMSGQTYPVTLFNRSAQGSIECRLT
ADVPYVKLSRKEVTVNGTQDETVYVSIDWDQAPNGRKRTEFSVSSPGMKPVKIAVETVKA
DAPALPDEKYRYYGSLADAIVIPAAGYTKKNGVNGVNWESIPELGRWNGFGAMAVIPALT
PSVLPPAAAPTLEYNVYIAEPGDVTVDVEILPIFPDKDDTGLQGASGYRELRIGTAFDDD
AVKVTDTLSREFKRLNNEIFENVRKVRVKHNVKNAGLHTFKVVMVDPAAAVERIVVYPER
VRRSYLGAPFNPMLPAGK

Enzyme Prediction     

EC	3.2.1.131

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH115	41	751	2.1e-231	0.9483500717360115

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam15979	Glyco_hydro_115	4.16e-169	186	525	1	334	Glycosyl hydrolase family 115. Glyco_hydro_115 is a family of glycoside hydrolases likely to have the activity of xylan a-1,2-glucuronidase, EC:3.2.1.131, or a-(4-O-methyl)-glucuronidase EC:3.2.1.-.
pfam17829	GH115_C	7.53e-33	800	969	9	171	Gylcosyl hydrolase family 115 C-terminal domain. This domain is found at the C-terminus of glycosyl hydrolase family 115 proteins. This domain has a beta-sandwich fold.
pfam03648	Glyco_hydro_67N	2.44e-07	51	152	21	119	Glycosyl hydrolase family 67 N-terminus. Alpha-glucuronidases, components of an ensemble of enzymes central to the recycling of photosynthetic biomass, remove the alpha-1,2 linked 4-O-methyl glucuronic acid from xylans. This family represents the N-terminal region of alpha-glucuronidase. The N-terminal domain forms a two-layer sandwich, each layer being formed by a beta sheet of five strands. A further two helices form part of the interface with the central, catalytic, module (pfam07488).

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRQ50360.1	6.00e-287	49	969	26	964
EEC55419.1	1.37e-286	49	969	51	989
QDW23484.1	6.13e-267	26	978	26	992
ARS36448.1	1.44e-254	5	969	23	972
QCR25177.1	1.15e-252	33	969	16	937

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4ZMH_A	7.57e-216	30	972	3	934	Crystalstructure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40],4ZMH_B Crystal structure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40]
4C90_A	6.14e-156	24	816	29	815	Evidencethat GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C90_B Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C91_A Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C91_B Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus]
7PUG_A	1.47e-147	42	649	17	636	ChainA, xylan alpha-1,2-glucuronidase [uncultured bacterium]
7PXQ_A	2.46e-144	42	649	16	635	ChainA, xylan alpha-1,2-glucuronidase [uncultured bacterium]
6NPS_A	6.06e-138	62	967	31	959	Crystalstructure of GH115 enzyme AxyAgu115A from Amphibacillus xylanus [Amphibacillus xylanus NBRC 15112],6NPS_B Crystal structure of GH115 enzyme AxyAgu115A from Amphibacillus xylanus [Amphibacillus xylanus NBRC 15112]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000912	0.997982	0.000285	0.000332	0.000252	0.000214

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003627_01147.