CAZyme Information: MGYG000003643_01507

Basic Information

• Species: W1P29-020 sp900771325
• Lineage: Bacteria; Verrucomicrobiota; Kiritimatiellae; RFP12; UBA1067; W1P29-020; W1P29-020 sp900771325
• CAZyme ID: MGYG000003643_01507
• CAZy Family: GH31
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1092	MGYG000003643_160|CGC1	120740.71	5.1049

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003643	2426387	MAG	Fiji	Oceania

• Gene Location: Start: 17557; End: 20835 Strand: +

Full Sequence      

MKQAVFGALALMAAGAFALTPGKVLKMVPQSGVSKSFDLITENGITFRVQFYTPQVFRIL
AAPQKLEPQLDRAGNPLKDKDGNVMTYVSADFSDPLNNPEKAQILVGVPEDKTQVRFADN
RAEGTITFATSAVTLTVNKADTTFSLAYSEGTPIWSEAAPLDLSANGTTQTLKTNADEFF
YGGGQQNGYLSHKGRKIDIRADGNWGEGGHPNPAPWYMTNTGYGVLRHTFSQGVYDFSAE
DAIKTTHYENRFDAFYFVGTNFAGTLNLYTQFTGRPNLIPRWGLQLGDADAYITRDEATK
DPAQNEDGTFKEVTPVDAVKVAKAYRENDMPGGWLLVNDGYGCGHMQLGYTVDALANLGF
YTGLWTEGALDRIKWEVGTAGTRVQKIDVAWSGPAYQHALECNKVAADGIEENADARAFV
WTVQGWAGTQRYGICWTGDQSGSWDLIRYHIPTLTCSSMSGQAYATTDVDGIFGGSNETF
LRDLQWKCWTTAIYVMNGWSHVNKGPWSYPEPYKSLNRKALLHKNRIVPYVYTYLAKAYD
SGAPVVRPLVYNYPEDRTTWGEETKYQFILGDDFLVAPVYNSMKLNKGWKKNIYLPEGLW
IDYRDGRRVQGPVMLPAVPITLEDIPVFVRAGAIVPMYPEMLVLGDKPFDPITLDLYPNP
AGATDTFVLYEDDGETQAHRYGQSSTQTFTLTSRSDERAGYIRVELGKIEGYYKGMPEAR
GYELIVHTRAKPTAVVFQNDDGTLGQEILPLCGEKAATTYANSRQAWYYDPEEKFGTLRI
KLAKMPVEAAARVLITTAPFSTIQATPAYPVPEVTDEIDKADFTATVNSAAADCPFALAL
DGTEETMWHSYWQEDAKQPTNFPYVAEIDMHGLYAINAVTYLPRANLGNGVVKGYKVEVA
RFPGKFTQVAEGELEPFTKAETRAIAFPSTWARYIRLTFTSAQRGEKFGSAAEIDVKQDL
HAKPLPDETKPIGHETVSVDGTDYPNSRRDQVGSDWTLDLDGTWEKIKGHAGMATANANQ
GTVTFRITADGKEIFARVGMKPGDVKQLIDVEIPAGAKQLRFTLKNESPDAIGSNTGVWT
DLKFFRAGSGRD

Enzyme Prediction     

No EC number prediction in MGYG000003643_01507.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH31	398	635	3.5e-60	0.5409836065573771

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06596	GH31_CPE1046	5.40e-166	273	606	2	334	Clostridium CPE1046-like. CPE1046 is an uncharacterized Clostridium perfringens protein with a glycosyl hydrolase family 31 (GH31) domain. The domain architecture of CPE1046 and its orthologs includes a C-terminal fibronectin type 3 (FN3) domain and a coagulation factor 5/8 type C domain in addition to the GH31 domain. Enzymes of the GH31 family possess a wide range of different hydrolytic activities including alpha-glucosidase (glucoamylase and sucrase-isomaltase), alpha-xylosidase, 6-alpha-glucosyltransferase, 3-alpha-isomaltosyltransferase and alpha-1,4-glucan lyase. All GH31 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein.
COG1501	YicI	8.48e-60	166	716	141	747	Alpha-glucosidase, glycosyl hydrolase family GH31 [Carbohydrate transport and metabolism].
pfam01055	Glyco_hydro_31	9.31e-53	403	635	215	442	Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases.
cd06603	GH31_GANC_GANAB_alpha	4.14e-45	417	676	213	467	neutral alpha-glucosidase C, neutral alpha-glucosidase AB. This subgroup includes the closely related glycosyl hydrolase family 31 (GH31) isozymes, neutral alpha-glucosidase C (GANC) and the alpha subunit of heterodimeric neutral alpha-glucosidase AB (GANAB). Initially distinguished on the basis of differences in electrophoretic mobility in starch gel, GANC and GANAB have been shown to have other differences, including those of substrate specificity. GANC and GANAB are key enzymes in glycogen metabolism that hydrolyze terminal, non-reducing 1,4-linked alpha-D-glucose residues from glycogen in the endoplasmic reticulum. The GANC/GANAB family includes the alpha-glucosidase II (ModA) from Dictyostelium discoideum as well as the alpha-glucosidase II (GLS2, or ROT2 - Reversal of TOR2 lethality protein 2) from Saccharomyces cerevisiae.
PLN02763	PLN02763	6.27e-25	413	714	388	669	hydrolase, hydrolyzing O-glycosyl compounds

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AQT69618.1	0.0	21	1089	36	1078
AKU23144.1	8.05e-225	38	1051	40	1060
BBB68104.1	9.76e-222	35	972	91	986
AZQ12882.1	4.10e-213	130	1074	117	1096
ABL98368.1	5.77e-213	36	1051	52	1076

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6M76_A	1.14e-103	154	735	149	785	GH31alpha-N-acetylgalactosaminidase from Enterococcus faecalis [Enterococcus faecalis ATCC 10100],6M77_A GH31 alpha-N-acetylgalactosaminidase from Enterococcus faecalis in complex with N-acetylgalactosamine [Enterococcus faecalis ATCC 10100]
7F7R_A	5.73e-103	154	735	149	785	ChainA, GH31 alpha-N-acetylgalactosaminidase [Enterococcus faecalis ATCC 10100]
7F7Q_A	1.51e-102	154	735	149	785	ChainA, GH31 alpha-N-acetylgalactosaminidase [Enterococcus faecalis ATCC 10100]
6JR8_A	4.46e-40	166	735	143	784	Flavobacteriumjohnsoniae GH31 dextranase, FjDex31A, mutant D412A complexed with isomaltotriose [Flavobacterium johnsoniae UW101],6JR8_B Flavobacterium johnsoniae GH31 dextranase, FjDex31A, mutant D412A complexed with isomaltotriose [Flavobacterium johnsoniae UW101],6JR8_C Flavobacterium johnsoniae GH31 dextranase, FjDex31A, mutant D412A complexed with isomaltotriose [Flavobacterium johnsoniae UW101],6JR8_D Flavobacterium johnsoniae GH31 dextranase, FjDex31A, mutant D412A complexed with isomaltotriose [Flavobacterium johnsoniae UW101]
6JR6_A	7.84e-40	166	735	143	784	Flavobacteriumjohnsoniae GH31 dextranase, FjDex31A [Flavobacterium johnsoniae UW101],6JR6_B Flavobacterium johnsoniae GH31 dextranase, FjDex31A [Flavobacterium johnsoniae UW101],6JR6_C Flavobacterium johnsoniae GH31 dextranase, FjDex31A [Flavobacterium johnsoniae UW101],6JR6_D Flavobacterium johnsoniae GH31 dextranase, FjDex31A [Flavobacterium johnsoniae UW101],6JR7_A Flavobacterium johnsoniae GH31 dextranase, FjDex31A, complexed with glucose [Flavobacterium johnsoniae UW101],6JR7_B Flavobacterium johnsoniae GH31 dextranase, FjDex31A, complexed with glucose [Flavobacterium johnsoniae UW101],6JR7_C Flavobacterium johnsoniae GH31 dextranase, FjDex31A, complexed with glucose [Flavobacterium johnsoniae UW101],6JR7_D Flavobacterium johnsoniae GH31 dextranase, FjDex31A, complexed with glucose [Flavobacterium johnsoniae UW101]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9P999	1.41e-33	404	710	392	692	Alpha-xylosidase OS=Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) OX=273057 GN=xylS PE=1 SV=1
Q8BHN3	3.22e-31	398	743	575	923	Neutral alpha-glucosidase AB OS=Mus musculus OX=10090 GN=Ganab PE=1 SV=1
Q14697	5.59e-31	398	743	575	923	Neutral alpha-glucosidase AB OS=Homo sapiens OX=9606 GN=GANAB PE=1 SV=3
Q4R4N7	9.71e-31	398	743	575	923	Neutral alpha-glucosidase AB OS=Macaca fascicularis OX=9541 GN=GANAB PE=2 SV=1
P79403	2.22e-30	407	743	584	923	Neutral alpha-glucosidase AB OS=Sus scrofa OX=9823 GN=GANAB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000301	0.998971	0.000170	0.000181	0.000176	0.000154

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003643_01507.