CAZyme Information: MGYG000003658_00066

Basic Information

• Species: CAG-110 sp900546915
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Oscillospiraceae; CAG-110; CAG-110 sp900546915
• CAZyme ID: MGYG000003658_00066
• CAZy Family: GH85
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2305	MGYG000003658_4|CGC1	246227.74	4.0693

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003658	2415548	MAG	United States	North America

• Gene Location: Start: 267; End: 7184 Strand: +

Full Sequence      

MKLLKRAVSALCAAALLAGTFAGVTKPVSAAGAVLGYWPEPLEIPNEYYYENETLQPYGS
CFQIDELKNWSPDNDPDARYNRSAIPLADRWMGPNVNPLASRDAKVMPLAMANARASQAP
SQGGDGDSAYVFNNFQYVDIFNFWGGSSSEGPIAIPSPEIIDAAHRNGVPATGTIFLPWG
DSTYGDRFVREMVEQDEDGRFIAADKLVEIAQYYGFDGYIFNAESGTGVAGFKEFLEYLQ
EIKPDNFTITWYNGSGSLSTGSIQSWMQDGDTRVTDHWWLDMSGNGYVDSTIQAAQQTGV
NPWNIHSTWEYWPMSGMSGVKGGNYQARLDENGILKCSLGILAPTCTLTQATSSEDFLNN
QDQKLWVGPTYDPSSTYRPTNEFCGFASLVADQTPVIGTDFVTNFTAGNGYRFYENGEVV
GKEDGWYNRSLTDVLPTWRWIIESEGQKLDAVIDYADAWYAGTSMKLSGSMDAGKSNHVK
LYSAQLEITKDSEFSMTYKTPVAGVQVELGLCFGDTYDAENFKFYPLTTTADGQWNTTTI
DLSEDAGKTAIAISLRFTAPDGVEDYAVNVGQMAFTTDRTAPEATSGVTLDEVIYPTDKT
MEARVYWEKAENAFMYRIYRVHTDGTREFVGATPSDAFYLGSYEKEAGENACTFEIVSYS
ENFVKGGTQTFSIAWPEEVEDGFVPVFEEGENLALKQPAISSVTCVADGPVHQLNDGTVL
NGSKWCSNSTQGYAVIDLGENKTIQRWVVYHANCPGAGESVDMNTVDFDFQYAPDDGGAL
LDPDDSASVERVKRLAYTVADAVTGNKQDVTDRNLDEPITARYIKLNVTKSDNSAWKAIR
IYEFEVYEKAAINNTPSPYERNVTVKNREGAADTVVVDNVPMQYSSGTYSDKNGVFHEDT
GKVRLFTDLTSEEPIAVVKAVQPDESYKQRGVGIATFEGLELNPEGGRLFYDVLDGSGPE
VTNSRRASVYYAPETGTPSQAPATVTLSRTTGGVQLRKQYATLTATGLEEGATMKIFASA
DAQTPILHTLPAVNGVITQTRVPLDPEGGTVYYEIYKEGKPDSERYALEYGDPMELAADL
TGLQELIAKCETIRQSECTSATWTAFAEKLDAARTVAAGTPKAAEAEAARAALNEAYAQL
RFKGNHQRLDELCREFEAAYPETDYTVGSFAKFQTALQAAQAAISADDSSNYQLEQLRID
LEAAVRGLVKQSEVSVTRVTVTPETTQVLPGGSVQFTARVEGTGDPSQEVTWSLEGQTST
ETALTDGGLLSVGRDEPAGAQLTVYAASVEDSTKTARATVTVVEDVEPTEPTDPSEPTIP
GLDEDTNVALDATVVAYNGPEAGSSMNGALGGNSGPEKLFDGAYANADTDKWCVDGDNMW
VAFDIGEEKDVARAILYHAGVANEYPPEKINTSDFQLYTLNTAKISVEDLLAKSFAERST
ILADNSYWTLVADVSGNTSNITTHDFETENARIFKLNVSKTDTAGWAACVRIYELELYAY
QESSVPGVEITVTPSELRVDGNPGQKGQFTAVVTGTDDVAVTWSISGNTSAQTLIENGQL
FFGADETAKVMTVTATSHADPTKQASATVYVEDETYSITISIDIAHGTVTADRAEAAEGE
QVTLTVTPEAGYVLKPGSLQVNGVPVEGTTFVMPGEDVVITAEFISDETEALLAAAQAAA
QAAEDAQEQAEAAQAAAEAAEQAAREAAASAAEDKAAAEAARAAAETAKAKAEAAQAAAE
AAREAAEDAASAAQASNLAAAQEAAKAAEAAAQAAGSATEAAQSAQAAAEAAEAAQEARE
AAEAAQARAEAAQKAAEEAAGAAGEDRTAAQAAKAKAEEAQRQAEEAAQAAEQAWTAAEE
SAAAAKEAQLAAAQEAQKAAEAAAQAAVSAAEAAEAAARAAQAMEKAQAAQAAAEAAAQV
AEEAAEKAAEAQKRAEEAAESSAENQEAAQRAAQEAEEAREAAQAARKAAEEAQTAAETS
RQAAEAANLEAAEAAAEAAEYARKVAETYGEIVEIKAEMVEFLAEAQKAAEEAEAAQKAA
EEAQKKAEEAALASTKYRALLDLATYADRDDYARPQQKLLAAAIQAGRDAIQAAADIPEV
EEALAGAKKAIDAIPTRAELEAKELPYTDVKEGAWYYQAVQYAVNQGLFQGTTESTFSPN
GTMSRAMAVTVLYRLAGSPEVEGTVDFTDVDAEAYYYRALVWAWEAGIVQGVDAERFAPQ
SMVTREQLVSLLYRYAQTQNMDVSEKADLGDYADAEQVSAYAREAMAWAIGNGIVEGVDA
ATLAPKGTATRAQAAAILMRFLENF

Enzyme Prediction     

No EC number prediction in MGYG000003658_00066.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH85	111	414	2.4e-58	0.9968253968253968

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG4724	COG4724	2.20e-128	56	576	28	550	Endo-beta-N-acetylglucosaminidase D [Carbohydrate transport and metabolism].
pfam03644	Glyco_hydro_85	9.53e-73	123	410	1	291	Glycosyl hydrolase family 85. Family of endo-beta-N-acetylglucosaminidases. These enzymes work on a broad spectrum of substrates.
cd06547	GH85_ENGase	2.48e-68	106	446	1	339	Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the protein and N-acetylglucosamine 2 forms the reducing end of the released glycan. ENGase is a key enzyme in the processing of free oligosaccharides in the cytosol of eukaryotes. Oligosaccharides formed in the lumen of the endoplasmic reticulum are transported into the cytosol where they are catabolized by cytosolic ENGases and other enzymes, possibly to maximize the reutilization of the component sugars. ENGases have an eight-stranded alpha/beta barrel topology and are classified as a family 85 glycosyl hydrolase (GH85) domain. The GH85 ENGases are sequence-similar to the family 18 glycosyl hydrolases, also known as GH18 chitinases. An ENGase-like protein is also found in bacteria and is included in this alignment model.
pfam00395	SLH	3.79e-07	2127	2166	1	40	S-layer homology domain.
pfam18998	Flg_new_2	4.94e-07	1596	1664	1	70	Divergent InlB B-repeat domain. This family of domains are found in bacterial cell surface proteins. They are often found in tandem array. This domain is closely related to pfam09479.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ASM70437.1	0.0	23	1210	22	1212
AMN32287.1	5.28e-189	45	1060	36	1019
SQI03683.1	7.28e-189	45	1060	36	1019
AMN35046.1	2.65e-188	45	1060	36	1019
ASY50962.1	5.04e-188	45	1060	36	1019

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3GDB_A	6.82e-110	53	841	156	937	Crystalstructure of Spr0440 glycoside hydrolase domain, Endo-D from Streptococcus pneumoniae R6 [Streptococcus pneumoniae R6]
2W91_A	3.74e-91	53	685	5	644	Structureof a Streptococcus pneumoniae family 85 glycoside hydrolase, Endo-D. [Streptococcus pneumoniae TIGR4],2W92_A Structure of a Streptococcus pneumoniae family 85 glycoside hydrolase, Endo-D, in complex with NAG-thiazoline. [Streptococcus pneumoniae TIGR4]
2VTF_A	5.48e-85	57	673	11	621	X-raycrystal structure of the Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae E173Q mutant reveals a TIM barrel catalytic domain and two ancillary domains [Glutamicibacter protophormiae],2VTF_B X-ray crystal structure of the Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae E173Q mutant reveals a TIM barrel catalytic domain and two ancillary domains [Glutamicibacter protophormiae]
3FHQ_A	8.87e-85	57	673	6	616	ChainA, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_B Chain B, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_D Chain D, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_F Chain F, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae]
3FHA_A	1.20e-84	57	673	6	616	ChainA, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_B Chain B, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_C Chain C, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_D Chain D, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A1L251	6.07e-26	40	574	44	533	Cytosolic endo-beta-N-acetylglucosaminidase OS=Danio rerio OX=7955 GN=engase PE=2 SV=1
P0C7A1	1.59e-20	49	475	72	467	Cytosolic endo-beta-N-acetylglucosaminidase OS=Gallus gallus OX=9031 GN=ENGASE PE=1 SV=1
Q8BX80	6.34e-20	34	515	54	509	Cytosolic endo-beta-N-acetylglucosaminidase OS=Mus musculus OX=10090 GN=Engase PE=1 SV=1
Q8NFI3	1.00e-18	34	515	62	517	Cytosolic endo-beta-N-acetylglucosaminidase OS=Homo sapiens OX=9606 GN=ENGASE PE=1 SV=1
P38537	1.49e-18	2138	2304	45	208	Surface-layer 125 kDa protein OS=Lysinibacillus sphaericus OX=1421 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000425	0.998755	0.000272	0.000221	0.000167	0.000139

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003658_00066.