CAZyme Information: MGYG000003658_00464

Basic Information

• Species: CAG-110 sp900546915
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Oscillospiraceae; CAG-110; CAG-110 sp900546915
• CAZyme ID: MGYG000003658_00464
• CAZy Family: GH85
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2202	MGYG000003658_14|CGC1	237604.34	3.951

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003658	2415548	MAG	United States	North America

• Gene Location: Start: 34518; End: 41126 Strand: +

Full Sequence      

MKETCLKRLLSFALVIAMVVSLCPTMVFAGDTLPYRGESALGENEPTSHGYRAEDLLSWD
PATDPYADLLRARVPLQNRIEAVAATQANPELNPEIQYLTLAGDYGNAFFGGTSYTNEFS
EYCFNFWQYIDYYAPWHGQTTATTPIELWDADGERKGTSHWEMRAFEFGMMNLPNAAYTN
AAHKNGVLSLGCIFLPRAYQSWRTLIQRDEEGNFPYADKLVEIAEYYGFDGWFVNLEGSD
TPTGEAKTELAAMLKYMRDKGQYIQYYNAGGSIDTQMMDAGAADSFFKDYGWSTSGAISE
VEKYGIAAVHGGFEAGGNRWTNNFSKMWSNDQVVCSIATLGTDFVHAGLDEDFGNYLDLR
RETDEFQWMSAVRERMWFTGSSGNPTDSSTSTNSEVGVSRNSMPGIASMITERSVVSGDT
FYTNFNTGHGLVYAEDGVISNEHEWANVNIQDILPTWQFWFETAGTELQAEYDYGTKYKK
ALTGTWSEGEQQGDFTYGEFDFDLVDPYEGGSSLAVYGKVDADNYMHLYKTDLEVTAETQ
MQVTFQKTSADDVAMSLGLVFAKDIDTEHNTYTVTDIPLENTTEVSDGWITATVDLSAFA
GEQVAVIGLNFKGTADNYQMHIGSIYYTSGRDLTPDAPTGLTIDKAYDTNEMVISWDLGD
YDTVKQYNVYAVLDGVEMYMGGIYDEIYYIKDLYDADGEVTVKVTAVGADGSESEAASVT
YDYSKAVSDVKVETADGVVRVSWTPADPAAATQLSLYVPMNGETYTATAAAGETSVEIAV
PTGADADGKAFVLTIAPEGGAVKTYDGELDDSYCIPYDAPMTNHKLTSPVACTDWYTLEL
TYTTESGASSTTTYTRGKKSHGETNNDWSRFQSLPNDIATLVVTLTDYDGNVSAPVSYLF
VDGEPVDVNREVDEEYIPDPALLQAVREQIGTTLGELMEYTGGLDCSGLEVSDLTGLTLI
SGLTDLNLSGTPVQELTSSMIPGNLKTLDLTGCTGLVSVELEQYPDLAVTFTGCSALETL
ELDGTHMTVVDLTGCTALKTLSMQNAETESVNLSSCVNLENLDLSGAQTAELDITKLTRL
HNFDLSNSAVEAIVAADAASYTNVYRWNWENAKLDLSDNTAEGKLKAGIADYFATHEVPD
EVSQEGTQVGSGDLDSWSGGTLNLTLSSECFLADVTVTNPYSSYGWYDNLTQFSVAVSVD
GEVYEEVAELSGITTDTATLQMPEGTRAKYVRITSTDTGDYYSWFTVNGYLVAEKGFTYG
GQKPATERDAVKDLQVIADGTTYQLLDLLTESYASTHTRSGNLLRDLTDADWIDSSYVTQ
QITAPKGVTVAITDAEGGAYVPEVEGPSLGVLDDTTNVALNATPLAASGSSSYSEGPEKL
FDGLSATKWCTSGTSGWLGFALDEPAIVGQWHTLNAGSEGAQYITVGCRLQVLNPESGIT
DEDFLSMSTTEQRNILRNSSYWKDLSAYADNTENEVTVDVDQENLTSAQVYRFYVDKAMP
GSTYGAVRVYELELYAYTGELGANINGVFVADTVGQYHASFIKAKEEIASAEIHVAVPTV
QDVEKLIDAIGTVTPDSKEALRAARTAYENLPEDQKELVGNYEKLVKAEAAYLALADAVE
KLIDAIGFVTLDSKEAIEAARTAYDALNDELKAMVSNYETLQKAEQAYALLTETEDLMEQ
AKAAQEAAEAAQAAAEAAQAAAEAAQQAAQEAASSAAEDQAAAQAAQVKAAEAQAKAEAA
QAAAEAAQTAAEQAAAAAEASNQAAAAAAAQAAEEAQKAAEEAVAAAKSAAQAAEAQAKA
QQAQEAAEAAARTAAEAQAKAEEAQKKAEEAADSTSEDQEAAEQAAREAEAAKQAAENAQ
AAAEDARKAAETAKQAADEANREAAESAALAAEYAKKVAETYAEIVQIKTELIERLAEAQ
EAADRAEEERKAAEEARKAAEEAQKKAEQAALNAAKYNAYTELAKVDTSECNAEQLELVQ
AILVETRQVVQDAATTEEVDAALEEAWKVIEEVMSLVCASDLFTDVKMGAWYHEGVDFMV
RGGYMEGVGNGLFGVNSRVTRGQMATILYRIAGEPGVEGLENPFRDLKNGSFYYDAVLWA
ASEGIVQGYEDGTYQPDAYISRQQLATILYRYSGQAKVEENVLSQYPDAGQVAGYAEEAM
NWAVANGFIRGVANGSVTTLNPASTATRAELSTILMRYLTEG

Enzyme Prediction     

No EC number prediction in MGYG000003658_00464.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH85	109	433	1.8e-51	0.9746031746031746

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG4724	COG4724	8.63e-70	44	625	27	547	Endo-beta-N-acetylglucosaminidase D [Carbohydrate transport and metabolism].
pfam03644	Glyco_hydro_85	2.12e-55	116	430	2	291	Glycosyl hydrolase family 85. Family of endo-beta-N-acetylglucosaminidases. These enzymes work on a broad spectrum of substrates.
cd06547	GH85_ENGase	2.18e-48	100	465	1	339	Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the protein and N-acetylglucosamine 2 forms the reducing end of the released glycan. ENGase is a key enzyme in the processing of free oligosaccharides in the cytosol of eukaryotes. Oligosaccharides formed in the lumen of the endoplasmic reticulum are transported into the cytosol where they are catabolized by cytosolic ENGases and other enzymes, possibly to maximize the reutilization of the component sugars. ENGases have an eight-stranded alpha/beta barrel topology and are classified as a family 85 glycosyl hydrolase (GH85) domain. The GH85 ENGases are sequence-similar to the family 18 glycosyl hydrolases, also known as GH18 chitinases. An ENGase-like protein is also found in bacteria and is included in this alignment model.
NF033190	inl_like_NEAT_1	8.86e-16	1986	2176	560	732	NEAT domain-containing leucine-rich repeat protein. Members of this family have an N-terminal NEAT (near transporter) domain often associated with iron transport, followed by a leucine-rich repeat region with significant sequence similarity to the internalins of Listeria monocytogenes. However, since Bacillus cereus (from which this protein was described, in PMID:16978259) is not considered an intracellular pathogen, and the function may be iron transport rather than internalization, applying the name "internalin" to this family probably would be misleading.
sd00033	LRR_RI	1.61e-10	947	1119	36	201	leucine-rich repeats, ribonuclease inhibitor (RI)-like subfamily. A leucine-rich repeat (LRR) is a structural protein motif of 20-30 amino acids that is unusually rich in the hydrophobic amino acid leucine. The conserved eleven-residue sequence motif (LxxLxLxxN/CxL) within the LRRs corresponds to the beta-strand and adjacent loop regions, whereas the remaining parts of the repeats are variable. LRRs fold together to form a solenoid protein domain, termed leucine-rich repeat domain. Leucine-rich repeats are usually involved in protein-protein interactions.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNO18499.1	8.84e-237	6	897	13	917
ALA43266.1	1.55e-187	27	817	31	829
APB74945.1	1.55e-187	27	817	31	829
AET57459.1	5.71e-187	27	838	31	854
AWB44945.1	1.02e-185	29	1000	36	1011

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2W91_A	1.24e-44	54	732	18	643	Structureof a Streptococcus pneumoniae family 85 glycoside hydrolase, Endo-D. [Streptococcus pneumoniae TIGR4],2W92_A Structure of a Streptococcus pneumoniae family 85 glycoside hydrolase, Endo-D, in complex with NAG-thiazoline. [Streptococcus pneumoniae TIGR4]
3GDB_A	1.42e-43	54	732	169	794	Crystalstructure of Spr0440 glycoside hydrolase domain, Endo-D from Streptococcus pneumoniae R6 [Streptococcus pneumoniae R6]
2VTF_A	1.29e-41	44	670	9	568	X-raycrystal structure of the Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae E173Q mutant reveals a TIM barrel catalytic domain and two ancillary domains [Glutamicibacter protophormiae],2VTF_B X-ray crystal structure of the Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae E173Q mutant reveals a TIM barrel catalytic domain and two ancillary domains [Glutamicibacter protophormiae]
3FHA_A	5.18e-41	44	704	4	597	ChainA, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_B Chain B, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_C Chain C, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_D Chain D, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae]
3FHQ_A	6.94e-41	44	704	4	597	ChainA, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_B Chain B, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_D Chain D, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_F Chain F, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P38537	6.11e-13	2001	2199	12	206	Surface-layer 125 kDa protein OS=Lysinibacillus sphaericus OX=1421 PE=3 SV=1
C6CRV0	8.62e-13	2023	2197	1283	1457	Endo-1,4-beta-xylanase A OS=Paenibacillus sp. (strain JDR-2) OX=324057 GN=xynA1 PE=1 SV=1
A1L251	1.20e-11	160	313	132	301	Cytosolic endo-beta-N-acetylglucosaminidase OS=Danio rerio OX=7955 GN=engase PE=2 SV=1
Q9SRL4	1.85e-08	160	562	99	478	Cytosolic endo-beta-N-acetylglucosaminidase 2 OS=Arabidopsis thaliana OX=3702 GN=ENGASE2 PE=1 SV=1
Q8BX80	1.90e-08	54	292	83	298	Cytosolic endo-beta-N-acetylglucosaminidase OS=Mus musculus OX=10090 GN=Engase PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000471	0.998632	0.000332	0.000208	0.000178	0.000156

TMHMM  Annotations     

start	end
9	28