CAZyme Information: MGYG000003679_00313

Basic Information

• Species: Treponema_D sp002449305
• Lineage: Bacteria; Spirochaetota; Spirochaetia; Treponematales; Treponemataceae; Treponema_D; Treponema_D sp002449305
• CAZyme ID: MGYG000003679_00313
• CAZy Family: GH13
• CAZyme Description: Pullulanase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
615		68526.1	6.4404

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003679	2427938	MAG	United Republic of Tanzania	Africa

• Gene Location: Start: 1075; End: 2922 Strand: +

Full Sequence      

MDKKYYSNENSASSIDSAFYYDGNDLGLTLNGTSASFKCWAPTASNVRLLLFKDSAAKKL
CAAKQMERKEKGVWFFSGSYEGCSYYQYEISFGAEIFKVADIWHTVASPDSVASQIAVSE
KSEYKNPFKETDYSKAVIYEMHIRDWSRTVNPSSTGKFLEAADPKIISHLKELGVTHVQI
LPMFDYAQKNSDLSYNWGYNPFHYNVPEGRYVSQGYTDGIQAVKEMQQMVKSFHDAGIAV
IMDVVYNHTDGTQNNSLYDMTVPKYFYRLNSEGGYSNGSGCGNEIATNHKMVKKYVIDSL
KHWMKDFHINGFRFDLMGVQEQETMSEIFCELKKIDPCVMVYGEPWTGGECAVKNGCSAS
VACGKNCGVGAFNDDFRDAIKGSEFGGFAKGHVQGIFCDSEIEKGLLGATGKNNRNPSGI
PSLSIHYVECHDNYTLFDKLAISYLEKISYSGNLYEAIGNVGLEKVKRQDILAAAYIILA
QGTPFINGGQEFLRTKQGNENSYNSSDEINQISLEFKMRYSDVFNAYKGLIAFRRKNPDS
FGSKTDCLAKTISPGLTKYTAGNFCIYFNATDSSAKIDSAGFANSIEVLSGKPEQKNFVP
DTVDAKSFVIFKKLD

Enzyme Prediction     

EC	3.2.1.41	3.2.1.1	3.2.1.-	3.2.1.68

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	167	491	2.4e-93	0.9965397923875432

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd11341	AmyAc_Pullulanase_LD-like	0.0	136	534	3	406	Alpha amylase catalytic domain found in Pullulanase (also called dextrinase; alpha-dextrin endo-1,6-alpha glucosidase), limit dextrinase, and related proteins. Pullulanase is an enzyme with action similar to that of isoamylase; it cleaves 1,6-alpha-glucosidic linkages in pullulan, amylopectin, and glycogen, and in alpha-and beta-amylase limit-dextrins of amylopectin and glycogen. Pullulanases are very similar to limit dextrinases, although they differ in their action on glycogen and the rate of hydrolysis of limit dextrins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase.
TIGR02104	pulA_typeI	2.79e-180	14	592	1	599	pullulanase, type I. Pullulan is an unusual, industrially important polysaccharide in which short alpha-1,4 chains (maltotriose) are connected in alpha-1,6 linkages. Enzymes that cleave alpha-1,6 linkages in pullulan and release maltotriose are called pullulanases although pullulan itself may not be the natural substrate. This family consists of pullulanases related to the subfamilies described in TIGR02102 and TIGR02103 but having a different domain architecture with shorter sequences. Members are called type I pullulanases.
COG1523	PulA	1.47e-125	19	539	61	590	Pullulanase/glycogen debranching enzyme [Carbohydrate transport and metabolism].
TIGR02103	pullul_strch	1.47e-99	12	512	112	726	alpha-1,6-glucosidases, pullulanase-type. Members of this protein family include secreted (or membrane-anchored) pullulanases of Gram-negative bacteria and pullulanase-type starch debranching enzymes of plants. Both enzymes hydrolyze alpha-1,6 glycosidic linkages. Pullulan is an unusual, industrially important polysaccharide in which short alpha-1,4 chains (maltotriose) are connected in alpha-1,6 linkages. Enzymes that cleave alpha-1,6 linkages in pullulan and release maltotriose are called pullulanases although pullulan itself may not be the natural substrate. This family is closely homologous to, but architecturally different from, the Gram-positive pullulanases of Gram-positive bacteria (TIGR02102). [Energy metabolism, Biosynthesis and degradation of polysaccharides]
PLN02877	PLN02877	1.03e-84	15	512	205	795	alpha-amylase/limit dextrinase

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AEB13374.1	0.0	1	615	1	615
QQA01551.1	1.98e-249	19	613	289	897
QSI02745.1	9.70e-235	26	613	265	878
QJS18678.1	6.38e-119	18	577	352	938
ACL70138.1	1.24e-118	19	538	21	544

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2WAN_A	1.34e-102	21	538	313	828	Pullulanasefrom Bacillus acidopullulyticus [Bacillus acidopullulyticus]
7LSA_A	2.80e-96	6	534	40	611	ChainA, Pullulanase [Ruminococcus bromii]
7LSR_A	4.09e-95	6	534	40	611	ChainA, Pullulanase [Ruminococcus bromii]
7LSU_A	4.26e-95	6	534	40	611	ChainA, Pullulanase [Ruminococcus bromii]
7LST_A	4.35e-95	6	534	40	611	ChainA, Pullulanase [Ruminococcus bromii]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O33840	1.23e-106	19	538	219	749	Pullulanase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=pulA PE=1 SV=2
C0SPA0	1.04e-88	13	538	94	617	Pullulanase OS=Bacillus subtilis (strain 168) OX=224308 GN=amyX PE=1 SV=1
P07811	3.70e-57	27	558	315	958	Pullulanase OS=Klebsiella aerogenes OX=548 GN=pulA PE=1 SV=1
Q8GTR4	6.30e-56	15	552	198	834	Pullulanase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PU1 PE=1 SV=2
P07206	2.79e-54	27	512	305	908	Pullulanase OS=Klebsiella pneumoniae OX=573 GN=pulA PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000061	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003679_00313.