CAZyme Information: MGYG000003684_00015

Basic Information

• Species: Eisenbergiella massiliensis
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Eisenbergiella; Eisenbergiella massiliensis
• CAZyme ID: MGYG000003684_00015
• CAZy Family: GH2
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
826	MGYG000003684_1|CGC2	95599.84	4.8082

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003684	6691925	Isolate	China	Asia

• Gene Location: Start: 19364; End: 21844 Strand: -

Full Sequence      

MTEEILDKNWKMKSLTDREFLETTVPGSVYQTYLAHGKMEDPFYRDNEEKALELMKEDFE
FITEFEVSDELLASDEILLHFDGIDTLGDISLNGKHLGKTDNMHRIWEFDVTDTVKKQGN
LLRIILFSPVNYIYDSYEQDPASSDCSDAMRGCSRLRKAHCMFGWDWGPRLPDAGIFRNV
KLLGIQDARIDNVYITQKHEKNCVTLSIQAEAVRAFGQIPFSPGSSDGDSEQYCYRVTIT
DPEGTAQTYDNSPAQILVENPKLWWPNGYGEQPLYRIRLELFRGQQLLDVWDRRIGLRTA
TVARKKDEWGESFAHEVNGVCIFAMGADYIPEDNVLPRVTPERTRRLLTQVRDAHFNCIR
VWGGGYYPDDAFYDICDELGLLVWQDFMFACCTYDLTKAFEESITAEFIDNIKRIRHHAC
LGLWCGNNEMEMFLAENVSGWCKKPGQLSDYFKMYEYIIPKVLEKYDPQTFYWPASPSSG
GGFDAPNDENRGDVHYWDVWHGDKPITEFRKFYFRYLSEFGFQSFPALKTVETFTLPEDR
NIFSYVMEKHQRNQSANGKIMNYLGQTYLYPADFGTLLYASQLLQAEAMRYGVEHFRRHR
GRCMGTLIWQLNDCWPTASWSMIDYEGRWKAVQYYAKRFFAPLLISCEEEGTLTQNTNVN
AEPFTLRKSVTLCASNETTQDQAAEIGWQLRHADGTCIREYREKAVIPALSSCWFQKTEL
PEARIHEEYVSYEMTVDGEIVSAGSVLFCAPKHFHFTDPHLRVEMAEEGILKIYASSFAK
SVEIKNGDDTLLLEDNYFDMNPGCRSIRVLEGKPENLQVRSVYDIR

Enzyme Prediction     

No EC number prediction in MGYG000003684_00015.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	6	698	1.6e-102	0.6595744680851063

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	8.26e-92	1	705	9	688	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	7.73e-13	76	429	127	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK10150	PRK10150	3.77e-10	58	429	66	417	beta-D-glucuronidase; Provisional
pfam00703	Glyco_hydro_2	1.53e-09	188	298	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
pfam17786	Mannosidase_ig	2.10e-08	670	753	3	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AEF82182.1	0.0	6	826	6	816
QJU13790.1	0.0	1	826	1	823
QBE97789.1	0.0	1	826	15	837
QIB54534.1	0.0	1	826	1	823
QMW79049.1	0.0	1	826	1	823

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7OP6_A	1.79e-146	5	825	12	842	ChainA, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2VJX_A	2.33e-146	5	823	10	838	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	2.45e-146	5	823	12	840	Structureof a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	4.72e-146	5	825	10	840	Structureof the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]
6BYE_A	2.07e-144	1	826	3	848	Crystalstructure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306],6BYE_B Crystal structure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q4WAH4	3.59e-112	6	803	9	821	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1
A1CGA8	7.00e-112	6	650	9	663	Beta-mannosidase B OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=mndB PE=3 SV=1
A1D911	9.79e-112	6	803	9	821	Beta-mannosidase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=mndB PE=3 SV=1
B0YBU9	1.37e-111	6	803	9	821	Beta-mannosidase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=mndB PE=3 SV=1
Q5B7W2	6.95e-111	2	650	5	663	Beta-mannosidase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mndB PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000047	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003684_00015.